ABSTRACT
Glucoside derivatives, xylarosides A (1) and B (2), were isolated from the broth extract of the endophytic fungus Xylaria sp. PSU-D14 along with two known compounds, sordaricin (3) and 2,3-dihydro-5-hydroxy-2-methyl-4H-1-benzopyran-4-one (4). The structures were assigned by spectroscopic methods. Sordaricin (3), one of the known metabolites, exhibited moderate antifungal activity against Candida albicans ATCC90028 with a MIC value of 32 microg/ml.
Subject(s)
Xylariales/metabolism , Glucosides/chemistry , Glucosides/metabolism , Magnetic Resonance Spectroscopy , Molecular Structure , Xylariales/chemistry , Xylariales/classificationABSTRACT
Chemical investigation of the wood-decayed fungus Xylaria sp. BCC 9653 has led to the isolation of a new methyl aminobenzoate (1) together with eleven known compounds. The structures were established by analysis of spectroscopic data. Cytochalasin D (2), one of the known metabolites, exhibited potent cytotoxicity against African green monkey kidney fibroblast (Vero) cells with an IC(50) value of 0.19 microM.
Subject(s)
Antiviral Agents/pharmacology , Cytochalasin D/pharmacology , Fibroblasts/drug effects , Kidney/drug effects , Wood , Xylariales/chemistry , para-Aminobenzoates , 4-Aminobenzoic Acid/chemistry , 4-Aminobenzoic Acid/isolation & purification , 4-Aminobenzoic Acid/pharmacology , Animals , Antiviral Agents/chemistry , Antiviral Agents/isolation & purification , Cells, Cultured , Chlorocebus aethiops , Cytochalasin D/chemistry , Cytochalasin D/isolation & purification , Fibroblasts/cytology , Inhibitory Concentration 50 , Kidney/cytology , Kidney/metabolism , Molecular Structure , Spectrum Analysis , Vero Cells/drug effectsABSTRACT
One new dihydrobenzofuran derivative, botryomaman (1), was isolated from the broth extract of the endophytic fungus Botryosphaeria mamane PSU-M76 along with six known compounds, 2,4-dimethoxy-6-pentylphenol, (R)-(-)-mellein, primin, cis-4-hydroxymellein, trans-4-hydroxymellein and 4,5-dihydroxy-2-hexenoic acid. The structures were assigned by spectroscopic methods. All compounds were tested for antibacterial activity against Staphylococcus aureus ATCC 25923 and methicillin-resistant S. aureus SK1. Primin showed the best activity against both strains with equal MIC values of 8 microg/ml.
Subject(s)
Anti-Bacterial Agents/isolation & purification , Anti-Bacterial Agents/pharmacology , Ascomycota/chemistry , Benzofurans/isolation & purification , Benzofurans/pharmacology , Chromatography, Thin Layer , Fermentation , Magnetic Resonance Spectroscopy , Methicillin Resistance , Microbial Sensitivity Tests , Spectrophotometry, Ultraviolet , Staphylococcus aureus/drug effectsABSTRACT
Five new chromones, named lachnones A-E (1-5), were isolated from the filamentous fungus Lachnum sp. BCC 2424 along with known (2E,6S)-2,6-dimethyl-2,7-octadiene-1,6-diol. Their structures were elucidated by spectroscopic methods. Lachnones B (2) and D (4) mildly inhibited the growth of Mycobacterium tuberculosis, both with MIC values of 200 microg/mL.
Subject(s)
Antitubercular Agents/isolation & purification , Ascomycota/chemistry , Chromones/isolation & purification , Mycobacterium tuberculosis/drug effects , Antitubercular Agents/chemistry , Antitubercular Agents/pharmacology , Chromones/chemistry , Chromones/pharmacology , Microbial Sensitivity Tests , Molecular Structure , Nuclear Magnetic Resonance, Biomolecular , ThailandABSTRACT
Tetraoxygenated xanthones, cowaxanthones A-E, together with 10 previously reported tetraoxygenated xanthones, were isolated from the crude hexane extract of the fruits of Garcinia cowa. Cowaxanthone B has previously been reported as a synthetic xanthone. Their structures were elucidated by analysis of spectroscopic data, especially by 1D and 2D NMR. The antibacterial activities of the isolated compounds were also evaluated.
Subject(s)
Anti-Bacterial Agents/chemistry , Fruit/chemistry , Garcinia/chemistry , Xanthones/chemistry , Anti-Bacterial Agents/isolation & purification , Anti-Bacterial Agents/pharmacology , Methicillin Resistance , Nuclear Magnetic Resonance, Biomolecular , Plant Extracts/chemistry , Plant Extracts/isolation & purification , Plant Extracts/pharmacology , Staphylococcus aureus/drug effects , Xanthones/isolation & purification , Xanthones/pharmacologyABSTRACT
Two pimarane diterpenes, named scopararanes A (1) and B (2), and two cytochalasins, named scoparasins A (3) and B (4), along with 4,8-dihydroxy-6-methoxy-4,5-dimethyl-3-methyleneisochroman-1-one (5) and diaportheins A (6) and B (7) were isolated from a culture broth of the endophytic fungus Eutypella scoparia PSU-D44. Their antimicrobial activities against Staphylococcus aureus ATCC 25923 and Microsporum gypseum SH-MU-4 were examined.
