ABSTRACT
N-acetyltransferase (NAT) and melatonin were determined in the optic lobe of the giant freshwater prawn Macrobrachium rosenbergii de Man. The prawns were divided into three groups: fast-growing "jumper" males; slow-growing "laggard" males; and females. Both NAT and melatonin levels in the jumper and laggard males were comparable, whereas those of the female were significantly lower. The results suggested a sexual dimorphism in the NAT and melatonin in the optic lobe of this species. It was also found that when one optic lobe was isolated, the level of NAT and melatonin in the contralateral optic lobe did not show a compensatory increase in either males or females. On the contrary, melatonin was suppressed in the remaining optic lobes in both sexes.
Subject(s)
Arylamine N-Acetyltransferase/analysis , Melatonin/analysis , Optic Lobe, Nonmammalian/chemistry , Palaemonidae/chemistry , Sex Characteristics , Analysis of Variance , Animals , Female , Male , Optic Lobe, Nonmammalian/enzymology , Palaemonidae/enzymologyABSTRACT
The effect of melatonin administration on nerve growth factor (NGF) was studied in the submandibular glands of adult Swiss male mice. Melatonin injection, at 1 microg daily for 30 days, resulted in an increase in the NGF content as detected by immunohistochemistry. The submandibular gland weight and the area of the granular convoluted tubules, which contained NGF, were also increased significantly. These effects were not observed when the dose of melatonin was increased to 10 and 50 microg daily. None of the melatonin treatments used influenced the weights or histology of the testes or seminal vesicles of the mice. The results suggest that melatonin, at physiological concentrations, directly regulates NGF synthesis in the mouse submandibular gland.
Subject(s)
Melatonin/pharmacology , Nerve Growth Factors/metabolism , Submandibular Gland/drug effects , Submandibular Gland/metabolism , Animals , Immunoenzyme Techniques , Male , Mice , Organ Size/drug effects , Submandibular Gland/cytologyABSTRACT
Giant freshwater prawns, Macrobrachium rosenbergii de Man, were reared under three different lighting conditions: continuous darkness (DD), 12 hr of light and 12 hr of darkness (LD 12:12) and continuous light (LL). After one month, the prawns were sacrificed and optic lobes isolated from the eyestalks were determined for N-acetyltransferase (NAT) activities and melatonin concentrations. Gonads were weighed and examined under light microscopy. The optic lobes from LL prawns contained significantly higher activities of NAT than those from LD 12:12 prawns. The melatonin concentrations and size and histological features of the gonads from the three groups of prawns did not differ. The results indicate that continuous light increases NAT activities in the optic lobe of M. rosenbergii but has no drastic effect on gonadal growth.
Subject(s)
Arylamine N-Acetyltransferase/metabolism , Light , Palaemonidae/enzymology , Animals , Darkness , Female , Gonads/anatomy & histology , Gonads/enzymology , Male , Melatonin/metabolism , Optic Lobe, Nonmammalian/enzymology , Organ Size , PeriodicityABSTRACT
Adult males of Schistosoma mansoni were preincubated in 5-hydroxytryptamine (5HT) and processed through the glyoxylic acid fluorescence technique to visualize 5HT-containing neural elements. 5HT fluorescence was seen as a series of cross-sectional rings beginning behind the ventral sucker toward the tail end. The rings were connected by two lateral nerve cords running longitudinally along the whole length of the worms.
