ABSTRACT
Introducción Los test de detección in vitro de Interferón-gamma frente a Mycobacterium tuberculosis (MTB) podrían ser una herramienta útil en el diagnóstico de enfermedad tuberculosa activa. Métodos Se realiza el test QuantiFERON-TB-Gold test in Tube (QFG-IT) en la sangre de 118 pacientes con tuberculosis pulmonar activa y se compara el resultado con la prueba de tuberculina. Resultados El estudio de QFG-IT fue positivo en 94 casos (79,7%), negativo en 17 (14,4%) e indeterminado en 7 (5,9%). QFG-IT negativo o indeterminado fue más frecuente en pacientes más ancianos (p=0,017) y en los casos de baciloscopia (..) (AU)
Introduction: The Interferon-gamma in vitro detection tests could be a useful tool in the diagnosis of active tuberculosis compared to Mycobacterium tuberculosis (MTB). Methods: The QuantiFERON-TB-Gold in Tube (QFG-IT) test was performed on the blood of 118 patients with active tuberculosis and the results compared with the tuberculin test. Results: The QFG-IT test was positive in (..) (AU)
Subject(s)
Humans , Tuberculosis/microbiology , Tuberculosis, Pulmonary/microbiology , Mycobacterium tuberculosis/isolation & purification , Tuberculin Test/methods , Interferon-gamma/isolation & purificationABSTRACT
INTRODUCTION: The Interferon-γ in vitro detection tests could be a useful tool in the diagnosis of active tuberculosis compared to Mycobacterium tuberculosis (MTB). METHODS: The QuantiFERON-TB-Gold in Tube (QFG-IT) test was performed on the blood of 118 patients with active tuberculosis and the results compared with the tuberculin test. RESULTS: The QFG-IT test was positive in 94 cases (79.7%), negative in 17 (14.4%) and indeterminate in 7 (5.9%). A negative or indeterminate QFG-IT test was more common in older patients (P=0.017) and in cases with negative smear tests (P=0.041). The kappa agreement between the tuberculin and QFG-IT tests was 74.5% with a kappa value of 0.45 (SE:0.136). Thirteen of the patients studied were infected with HIV and the tuberculin was positive in 5 of the 12 cases (38.5%) in whom it was performed, with the QFG-IT being positive in 9/13 (69.2%). CONCLUSIONS: The QFG-IT test may be a useful complimentary tool to the tuberculin test in the diagnosis of tuberculosis.
