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Nat Prod Res ; 34(7): 995-1001, 2020 Apr.
Article in English | MEDLINE | ID: mdl-30584781

ABSTRACT

Ethanol extracts of different parts of Passiflora cincinnata were obtained by maceration. The total phenolic and flavonoid contents were evaluated. The antioxidant activities were determined by ß-carotene-linoleic acid bleaching test, 2,2-diphenyl-1-picrylhydrazil (DPPH), and 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical scavenging. The crude ethanol stem extract showed the highest amount of total polyphenols (45.53 mg gallic acid equivalent/g) while the highest total flavonoid contents (1.42 mg of quercetin equivalent/g) were observed in the leaf extract. The lowest IC50 (25.65 µg/ml) by the DPPH method was observed for the stem extract. The ABTS method showed a significant antioxidant activity for all investigated extracts. The secondary metabolite composition of ethanol extracts was assessed by HPLC-DAD-MS/MS analysis, leading to the identification of fourteen secondary metabolites in P. cincinnata extracts. These results showed the potentiality of this species as a source of phenolic compounds and antioxidants.


Subject(s)
Antioxidants/chemistry , Flavonoids/analysis , Passiflora/chemistry , Phenols/analysis , Plant Extracts/chemistry , Secondary Metabolism , Antioxidants/pharmacology , Chromatography, High Pressure Liquid/methods , Flavonoids/chemistry , Polyphenols/analysis , Quercetin/analysis , Tandem Mass Spectrometry/methods
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