ABSTRACT
A cluster of methicillin-resistant Staphylococcus aureus (MRSA) breast abscesses in women who had given birth at a hospital in Mumbai, India was investigated retrospectively. Nineteen of 20 cases were caused by a single clone: pvl-positive, spa type 648 (Ridom t852), ccrB:dru subtype 3:0, ST22-MRSA-IV. Despite the presence of pvl and SCCmec type IV, which are common genetic markers in community-associated MRSA, this outbreak was caused by a healthcare-associated, community-onset MRSA that was common in the hospital environment. Thus, infection control practices may have an important role in limiting the spread of this virulent clone.
Subject(s)
Abscess/epidemiology , Disease Outbreaks , Mastitis/epidemiology , Methicillin-Resistant Staphylococcus aureus/classification , Methicillin-Resistant Staphylococcus aureus/isolation & purification , Staphylococcal Infections/epidemiology , Abscess/microbiology , Cluster Analysis , Female , Humans , India/epidemiology , Mastitis/microbiology , Methicillin-Resistant Staphylococcus aureus/genetics , Molecular Typing , Postpartum Period , Pregnancy , Retrospective Studies , Staphylococcal Infections/microbiology , Virulence Factors/geneticsABSTRACT
Kodamaea ohmeri is an extremely uncommon human pathogenic yeast. It causes opportunistic infection in immunocompromised hosts. We report a case of Kodamaea ohmeri fungemia in a preterm neonate who succumbed despite antifungal therapy.
Subject(s)
Fungemia/drug therapy , Antifungal Agents , Fatal Outcome , Humans , Immunocompromised Host , Infant, Newborn , Infant, Premature , Opportunistic Infections , Pichia/isolation & purificationABSTRACT
PURPOSE: To compare the rapid colorimetric nitrate reductase based antibiotic susceptibility (CONRAS) test performed on Mycobacterium tuberculosis isolates with the conventional method i.e, the proportion method. METHODS: One hundred clinical isolates of M. tuberculosis were tested for susceptibility to isoniazid (INH) and rifampicin (RIF) by the conventional proportion method and CONRAS in Middlebrook 7H9 liquid medium enriched with growth supplements (MB7H9S). RESULTS: The performance of the CONRAS test was evaluated using proportion method as the gold standard. The sensitivity (ability to detect true drug resistance) and specificity (ability to detect true drug susceptibility) of the CONRAS test to INH was 93.75 and 98.52% and for RIF it was 96.10 and 100% respectively. The mean time for reporting was 6.3 days and the test showed excellent reproducibility. The kappa (k) value for INH was 0.92 and for RIF was 0.99, indicating excellent agreement between the two methods. CONCLUSIONS: CONRAS test is a rapid and reliable method of drug susceptibility for M. tuberculosis.
