ABSTRACT
OBJECTIVE: To investigate antidiabetic efficacy of the extract of field grown and in vitro raised leaves of Solanum xanthocarpum (S. xanthocarpum) against alloxan induced diabetic rats. METHODS: The antidiabetic activity of the crude methanol extracts of the field grown and in vitro raised leaves of S. xanthocarpum at different concentrations (100-200 mg/kg bw) was tested against alloxan induced diabetic rats. The antidiabetic efficacy was validated through various biochemical parameters and the antioxidant effect was also determined. The phytochemical analyses of field grown S. xanthocarpum and in vitro rasied S. xanthocarpum leaves were done by estimating their chlorophyll, carotenoids, total sugar, protein, amino acid and minerals contents. RESULTS: The results revealed that the methanol extracts of both the leaves (field grown and in vitro raised) of S. xanthocarpum was efficient anti hyperglycemic agents at a concentration of 200 mg/kg bw and posses potent antioxidant activity. However, the extracts of in vitro rasied S. xanthocarpum raised leaves exhibit higher efficacy than the field grown leaves in all tested concentrations. Proximal composition and mineral analysis of S. xanthocarpum revealed higher concentration of contents in in vitro rasied S. xanthocarpum than field grown S. xanthocarpum. CONCLUSIONS: From the results it can be concluded that the leaves extracts of S. xanthocarpum can be a potential candidate in treating the hyperglycemic conditions and suits to be an agent to reduce oxidative stress.
Subject(s)
Diabetes Mellitus, Experimental/drug therapy , Hyperglycemia/drug therapy , Phytotherapy , Plant Extracts/therapeutic use , Plant Leaves/chemistry , Solanum/chemistry , Animals , Biomarkers/blood , Diabetes Mellitus, Experimental/complications , Hyperglycemia/etiology , Male , Oxidative Stress/drug effects , Plant Extracts/pharmacology , Rats , Rats, Wistar , Treatment OutcomeABSTRACT
Induction of micronuclei in five different species of fish from polluted sewage water and in fish exposed to heavy metals was investigated. The frequency of micronuclei was statistically significant in both the groups and among the five species tested, Lepidocephalus was found to be highly sensitive.
