ABSTRACT
Terahertz (THz) radiation was proposed recently for use in various applications, including medical imaging and security scanners. However, there are concerns regarding the possible biological effects of non-ionising electromagnetic radiation in the THz range on cells. Human embryonic stem cells (hESCs) are extremely sensitive to environmental stimuli, and we therefore utilised this cell model to investigate the non-thermal effects of THz irradiation. We studied DNA damage and transcriptome responses in hESCs exposed to narrow-band THz radiation (2.3â THz) under strict temperature control. The transcription of approximately 1% of genes was subtly increased following THz irradiation. Functional annotation enrichment analysis of differentially expressed genes revealed 15 functional classes, which were mostly related to mitochondria. Terahertz irradiation did not induce the formation of γH2AX foci or structural chromosomal aberrations in hESCs. We did not observe any effect on the mitotic index or morphology of the hESCs following THz exposure.
Subject(s)
DNA Damage/genetics , Embryonic Stem Cells/metabolism , Embryonic Stem Cells/radiation effects , Genome, Human , Terahertz Radiation , Transcription, Genetic/radiation effects , Cell Line , Cell Nucleus/metabolism , Cell Nucleus/radiation effects , Cell Shape/radiation effects , Chromosome Aberrations , Cluster Analysis , Cyclin B1/metabolism , Cytogenetic Analysis , DNA Breaks, Double-Stranded/radiation effects , G1 Phase/radiation effects , Histones/metabolism , Humans , Indoles/metabolism , Mitotic Index , Molecular Sequence Annotation , Phosphorylation/radiation effectsSubject(s)
Bacillus/radiation effects , Electrons/adverse effects , Escherichia coli/radiation effects , Laser Therapy/methods , Lasers , Radiation Injuries/etiology , Bacteriophage lambda/genetics , Bacteriophage lambda/metabolism , DNA, Viral/chemistry , Dose-Response Relationship, Radiation , Histocytochemistry , Laser Therapy/instrumentation , Models, BiologicalABSTRACT
A 100 MeV eight-turn accelerator-recuperator intended to drive a high-power infrared free-electron laser (FEL) is currently under construction in Novosibirsk. The first stage of the machine includes a one-turn accelerator-recuperator that contains a full-scale RF system. It was commissioned successfully in June 2002.
