ABSTRACT
Acute hepatic failure was induced in rats by galactosamine injection intraperitoneally (1 gm per kg). Twenty-four hours later rats were treated by hemoperfusion (HP) over encapsulated sorbents: cellulose acetate-coated charcoal, polyelectrolyte-coated XAD4, a combination of both, or cross circulation with a healthy donor. Compared with control treatment (prevention of hypoglycemia by glucose infusion), the survival rate was not improved by HP or cross circulation: controls 19% vs. treated animals 0 to 17%. Extension of duration or increased frequency of HP gave the same survival rates. Computer simulation based on zero-order introduction of a possible toxin into a two-compartment model shows that HP up to 5 hr per day is not able to clear the body effectively from the assumed toxin if its partition coefficient exceeds a value of 50.
Subject(s)
Cross Circulation , Hemoperfusion , Hepatic Encephalopathy/therapy , Parabiosis , Animals , Galactosamine , Hemoperfusion/methods , Hepatic Encephalopathy/chemically induced , Hepatic Encephalopathy/mortality , Male , Rats , Rats, Inbred Strains , Software , Time FactorsABSTRACT
The experimental setup, including instrumentation and software packaging, is described for the use of a minicomputer as an on-line analyzing system of the EEG in rats. Complete fast Fourier transformation of the EEG sampled in 15 episodes of 10 s each is plotted out within 7 min after the start of registration of the EEG signal. The influence of high voltage spikes, spindles and baseline drift on the power density spectrum is shown and a method of clipping is presented that excludes these kinds of artefacts. The normalized standard error of the power density estimate is determined by the smoothing procedure and is at maximum 26%.
Subject(s)
Computers , Electroencephalography/instrumentation , Minicomputers , Animals , Online Systems , RatsABSTRACT
During galactosamine-induced acute liver failure in white male Wistar rats, EEG was recorded at regular intervals. Spectral analysis was done as described by Kropveld and co-workers in 1983. The specificity of the anomalies in the power-density spectra is tested by comparing the changing patterns with those caused by several pharmaceutical agents--ether, diazepam, pentobarbital. Quantification of the observed anomalies is discussed.
