[Autokeratoplasty in perforation of the ectatic leukoma of the only functionally perspective eye]. / Autokeratoplastika pri perforatsii éktazirovannogo bel'ma edinstvennogo glaza.

Treatment and rehabilitation of patients with destructive lesions of the cornea is a serious problem for ophthalmologists due to severity of the outcome of the disease - the formation of leukomas with a persistent decrease in vision, or even a complete loss of visual functions. The article presents a clinical case of a patient with posttraumatic ectaticized leukoma of the functionally promising eye, which course was complicated by perforation. The authors, in the absence of the donor cornea, faced a complex clinical task - the risk of functional and anatomical death of the single eyeball in the absence of adequate care for the patient. After enucleation of the subatrophic blind right eye, we took a corneal specimen with a scleral rim of 12 mm trepan. To collect the material, we used an original method of introducing viscoelastic into the vitreal cavity of the hypotonic enucleated eyeball through the intersected optic nerve. This allowed to create the normotony of the enucleated eyeball and perform trepanation of the corneoscleral disc. Trepanation of the sharply ectatic cornea of the left eye was performed after paracentesis and massage of the dome of ectatic cornea with a spatula, which led to the 'subsidence' of the hyperinflated tissue. The corneoscleral disc was fixated with interrupted suture with a 9-0 thread. The postoperative period proceeded smoothly. The autograft has taken with active vascularization. For optical rehabilitation, the patient is scheduled to undergo a keratoprosthetic operation in a planned manner.

Activity of Heme Synthesis Enzymes in the Bone Marrow and Liver of August and Wistar Rats During the Neonatal Period and After Acute Postnatal Hypoxia.

Activity of heme synthesis enzymes in newborn August and Wistar rats was studied after acute hypoxic hypoxia. Daily production of erythrocytes and activities of aminolevulinate synthase, aminolevulinate dehydratase, and heme synthetase were measured in the bone marrow (15-30 min after birth and on days 1 and 3 of life) and liver (day 3 after birth). Hypoxia was followed by a decrease in activity of heme synthesis enzymes in the liver (especially in August rats) and reduction of the daily erythrocyte production (especially in Wistar rats). Our results suggest that the response of heme synthesis enzymes to hypoxic exposure in newborn rats is genetically determined. The observed changes are more pronounced in Wistar rats.

[NOCEBO EFFECT: AN ADVICE TO A PRACTITIONER].

Nocebo effect is the onset or exacerbation of unwanted symptoms (adverse reaction) as a reaction to medical treatment. The nocebo effect is very common both in clinical practice and in clinical trials. Medical practitioner's knowledge of the patient's predisposition to nocebo effects can affect the management strategy and building doctor-patient relationship. It allows prediction and prevention of nocebo effect, especially if the patient has negative clinical encounters in the past.There is a dilemma: to inform the patient about the side effects and complications of treatment, which may lead to the development of nocebo effect and adversely influence the patient health and treatment outcomes, or conceal the information, thus infringing the patient civil rights. Solving this contradiction and minimizing the risk of nocebo effect allows choosing the proper strategy of doctor-patient communication. It is important not to ignore the various psychological, medical and organizational measures aimed at improving the patient comfort in the course of treatment. This prevents the accumulation of negative treatment experiences and is the key to successful treatment and prevention of nocebo effect.

[ERCC1 as a Marker of Ovarian Cancer Resistance to Platinum Drugs].

The review is concerned with the crucial marker of nucleotide excision repair ERCC1 and its contribution to platinum resistance of ovarian cancer. All the variants of the laboratory and clinical ERCC1 assessment in the ovarian cancer tissue (single nucleotide polymorphisms of the ERCC1 gene, levels of mRNA or protein) are considered. Data on the prognostic and predictive value of ERCC1 as a marker of the response to platinum-based therapy in ovarian cancer are systematized. The authors discuss the possible causes of heterogeneity of the results and emphasize the necessity of a unified and integrated approach to evaluation of ERCC1 in the tumor. The publications cited in the Search Engine Pub Med up to January 2015 were analyzed.

Transformation of catalytic characteristics of cerebral monoamine oxidases in experimental posttraumatic stress disorders.

We studied the contribution of the transformation of the catalytic properties of cerebral monoamine oxidase in the development of behavioral disorders during stress. Monoamine oxidase activities and catalytic characteristics, LPO intensity, and oxidative modification of proteins in suspension of the brain mitochondria were evaluated over the course of experimental posttraumatic stress disorder. The detected shifts were comparable with the results of neuroethologic testing. The development of behavioral disorders presented by low exploratory activity and high anxiety was associated with transformation of catalytic characteristics of cerebral monoamine oxidases, associated with the development of oxidative stress with predominant intensification of metal-catalyzed protein oxidation.

Gut microbiota among children living in areas contaminated by radiation and having the cardiac connective tissue dysplasia syndrome. / Mikrobiocenoz kyshechnyka u ditej-meshkanciv radioaktyvno zabrudnenyh terytorij iz syndromom dyplazii' spoluchnoi' tkanyny sercja.

Objective. The study examined the gut (colonic) microbiota in children being domiciled in contaminated zones and suffering the cardiac connective tissue dysplasia syndrome (CCTDS). Materials and methods. The study included 99 children living in contaminated zones. Study subjects were divided into subgroup IA (a comparison subgroup) of 44 children with no signs of CCTDS and subgroup IB of 55 children having the CCTDS. The control group included 24 children aged from 7 to 17 years old. Study groups were of the same gender and age. Results. In the absence of any specific complaints the abnormal gut microbiota was revealed in children living in contaminated areas with a high incidence of 96.36 % featuring both quantitative and qualitative abnormalities that can be considered a dysadaptation phenomenon of both digestive system and body as a whole. Under the concomitant CCTDS these disorders are more expressive, being characterized by a significant decrease in the number of obligate gut flora and failure of its protective capabilities. Incidence of dysbacteriosis grade III in children having the CCTDS is significantly higher vs. children of the control group and comparison subgroup. Under CCTDS the gut microbiota abnormalities were represented with a severe bowel contamination by E. coli with altered enzymatic properties, various types of opportunistic microorganisms, and a high identification incidence of genus Candida fungi at the background of a significant depression of normal colonic flora. Presence of 3-5-component associations of opportunistic pathogens in the colon was found with high incidence. Conclusion. According to received results the examination of intestinal bacterial flora is expedient in children living in areas contaminated by radiation. Application of health care arrangements aimed at normalization of gut microbiota is obligate.

[Experimental studies with mice on the program of the biosatellite BION-M1 mission].

Purpose of the BION-M1 project was laying the evidence and technological basis for addressing the medical issues of future remote space exploration missions by humans. The program of researches with the use of mice was focused on elicitation of cellular and molecular mechanisms of the muscular, cardiovascular and immune reactions to extended exposure in microgravity. The comprehensive studies combined lifetime measurements with investigations of mice tissues and cells by dint of the cutting-edge morphological, biochemical and molecular biology techniques. Males of mice C57/BL6 aged 4 to 5 months were chosen as the object of studies. They were distributed into the flight, ground control and two vivarium (laboratory control) groups and investigated immediately on return and after 7 days of readaptation. Some of the physiological functions were recorded throughout the flight. To ensure wellbeing of the animals in the experiments and to enhance data quality, prior to launch the mice were specially trained so as to accommodate to the group living, eating space food, and in-flight stress factors. Those of the mice that were designated for lifetime investigations were tested and received training pre-launch.

Stress-associated cardiovascular reaction masks heart rate dependence on physical load in mice.

When tested on the treadmill mice do not display a graded increase of heart rate (HR), but rather a sharp shift of cardiovascular indices to high levels at the onset of locomotion. We hypothesized that under test conditions cardiovascular reaction to physical load in mice is masked with stress-associated HR increase. To test this hypothesis we monitored mean arterial pressure (MAP) and heart rate in C57BL/6 mice after exposure to stressful stimuli, during spontaneous locomotion in the open-field test, treadmill running or running in a wheel installed in the home cage. Mice were treated with ß1-adrenoblocker atenolol (2mg/kg ip, A), cholinolytic ipratropium bromide (2mg/kg ip, I), combination of blockers (A+I), anxiolytic diazepam (5mg/kg ip, D) or saline (control trials, SAL). MAP and HR in mice increased sharply after handling, despite 3weeks of habituation to the procedure. Under stressful conditions of open field test cardiovascular parameters in mice were elevated and did not depend on movement speed. HR values did not differ in I and SAL groups and were reduced with A or A+I. HR was lower at rest in D pretreated mice. In the treadmill test HR increase over speeds of 6, 12 and 18m/min was roughly 1/7-1/10 of HR increase observed after placing the mice on the treadmill. HR could not be increased with cholinolytic (I), but was reduced after sympatholytic (A) or A+I treatment. Anxiolytic (D) reduced heart rate at lower speeds of movement and its overall effect was to unmask the dependency of HR on running speed. During voluntary running in non-stressful conditions of the home cage HR in mice linearly increased with increasing running speeds. We conclude that in test situations cardiovascular reactions in mice are governed predominantly by stress-associated sympathetic activation, rendering efforts to evaluate HR and MAP reactions to workload unreliable.

Lipid peroxidation and state of the erythron in newborn rats under normal conditions and impairment of sensorimotor reflexes.

Specimens with a stereotypic sensorimotor deficit were revealed among newborn rats due to abnormalities in prenatal ontogeny and birth stress. Genetically determined differences in the total lipid content of blood plasma were found in Wistar and August rat pups. Hence, LPO processes were shown to differ in these animals. August rats with intense lipid metabolism were characterized by higher activity of LPO in comparison with Wistar rats. The increase in LPO was associated with a relatively low activity of antioxidant enzymes. Impairment of sensorimotor reflexes in newborn Wistar and August rats was accompanied by suppression of the erythron and activation of extravascular hemolysis.

[Cortisol levels in cord blood with various violations of neonatal adaptation].

Shifting extra uterine existence is a serious test of maturity of all functional systems of the newborn, so in recent years, the more attention is payed for the adaptation of the newborn. It is known that the pathological course of pregnancy is a violation of the functional state of the adrenal cortex, and prolonged exposure of the pathological factor leads to the inhibition of adrenocortical function and a decrease of the adaptive capabilities of a newborn. Therefore, serum cortisol levels could be a predictor of complications in preterm infants. Based on this, it seems appropriate to study changes in the content of serum cortisol cord blood prior to the development of various syndromes neonatal maladjustment. The clinical and laboratory examination of term infants with various disorders of adaptation: regurgitation, pain, hyperexcitability , depression, cardio-respiratory depression, bleeding in the skin, toxic erythema. It is shown that the most prominent violations maladjustment processes occur on the background of decreased cortisol. Border states, which are formed on the background of normal or elevated cortisol, are not accompanied by significant impairments of adaptation processes. On the basis of the data was drawn a conclusion about the possibility of using changes of cortisol in cord blood as a reliable indicator of neonatal adaptation processes, pointing to the possibility of the development of pathological conditions.

[Discordance of estrogen receptor status between primary and metastatic breast cancer--possible reasons and prognostic value].

The aim of the review was systematization of the data on discordance in expression of estrogen receptors between primary and metastatic breast cancer, different metastases and repeated analyses of the same tissue. The possible reasons for the phenomenon are discussed. The authors emphasize the need to analyze estrogen receptors in breast cancer metastases, regardless of the receptor status of the primary tumor, for predicting the course of the metastatic disease and providing an adequate treatment of the metastatic tumor in strict accordance with its receptor status during drug therapy. The works cited in the search engine Pub Med to May 2013 were analyzed.

[Condition of erythron in newbord rats with disturbed sensorimotor reflexes].

Early adaptation issues of newborn rats of Wistar population and August line were examined. Absence of the connection between the condition of a female during the pregnancy and the birth, and newborn rats in early postnatal period was revealed.

[The activity of platelet monoamine oxidase, red blood cell Mg-ATPase, and the level of serum magnesium in the acute period of adaptation in neonatal infants].

For early diagnosis of neonatal transient conditions that may become abnormal, it is promising to apply an integrative approach comprising the concurrent definition of the indices characterized by a high sensitivity to hypoxia. This study has investigated the predictive value of platelet monoamine oxidase and red blood cell Mg-ATPase in neonatal infants.

Variability of microsomal oxidation and porphyrin metabolism in rats.

An inverse relationship between erythropoiesis intensity and microsomal oxidation level has been detected during the early postnatal period in rats with high resistance to hypoxia.

[Effect of early postnatal hypoxia on Wistar and August rats].

It was proved that hypoxia tolerance often goes together with forming of oxidative stress tolerance and has genetic predetermination on hypoxic influence. Interline difference ofhypoxia sensitivity is mainly defined by difference of oxygen-transport systems state. Genetically predetermined differences between investigated lines give the August rats the ability to use mechanisms of resistant strategy more effectively, and the Wistar rats--to use tolerant strategy more effectively.

[Xanthine oxidase as an indicator of neonatal adaptation].

The reactions reflecting adaptation may assume pathological traits under certain conditions. It is necessary to have reliable predictors that characterize adaptive processes. As this criterion, it is expedient to use the most important marker of free radical oxidation, such as xanthine oxidase (XO) activity. On the basis of the authors' findings, it can be said that it is advisable to apply this test to the screening of the course of adaptation within the first days after birth and to the detection of neonatal infants who require more scrupulous attention.

[Diagnostic and prognostic possibilities of using the indicators of blood serum middle molecule and medium-molecular peptide levels in inflammatory diseases of the small-pelvic organs]. / Diagnosticheskie i prognosticheskie vozmozhnosti ispol'zovaniia poka- zatelei molekul srednei massy i srednemolekuliarnykh peptidov krovi pri vospalitel'nykh zabolevanniakh organov malogo taza u zhenshchin.

Analysis of the diagnostic and prognostic potentialities of measurements of blood serum medium molecules and medium-molecular peptides in women with inflammatory diseases of the small pelvis organs has been carried out, involving 60 patients and 10 healthy women. Medium molecule levels were estimated with the use of Gabrielyan and Lipatova's method, the level of medium-molecular peptides by the biuret method. The results recommend these measurements to be included in the complex of clinical laboratory investigations carried out in patients with pelvic inflammations for the early detection of purulent ++tubo-ovarian inflammations, endogenous intoxication, and a more rational treatment.