[Acid resistance of erythrocytes in children with chronic inflammatory diseases of the stomach and duodenum during the course of the hyperbaric oxygenation].

The use of additional methods of treatment of ulcers of the duodenum determines necessity of development of methods of evaluation and control of the efficiency of the treatment. With the help of the method of acid erhytrogrammes highlighted the most significant signs of describing the change in erythrocyte membranes of children with duodenal ulcer disease during the course of the hyperbaric oxygenation.

[The ingredients of the gastric and duodenum mucosa secretions in chronic gastroduodenitis in adolescents].

Number of children with chronic gastroduodenitis and duodenal ulcer, is increasing every year. A major role in the development of chronic inflammation of the mucous membrane of the gastroduodenal zone play cytoprotective properties of mucus produced blennogenic structures of these bodies. Therefore, the appointment of therapies to help improve the cytoprotective properties of gastric and duodenal ulcers, remains valid. Presents the results of the study lektingistohimicheskogo slizeobrazovaniya with chronic gastroduodenitis in adolescents.

[GABAergic modulation of the oscillatory activity in the medial septal area during epilepsy].

On brain slices from healthy guinea pigs and animals with a model of chronic temporal lobe epilepsy, a comparative study of GABAergic modulation of oscillatory activity of neurons in the medial septal area was carried out. Under the action of GABA, burst activity persisted only in pacemakers in both groups of preparations. In epilepsy, the effectiveness of GABA action on the rhythmic neurons sharply increased. In the control group, GABA significantly reduced bursts frequency in cells preserving their oscillatory activity, whereas in slices from the epileptic brain burst frequency increased under the action of GABA. Blockade of GABAergic receptors led to a disruption of tonic GABAergic intraseptal influences and to a significant decrease in the effectiveness of blockers in epilepsy. The study was the first to demonstrate a dysfunction of the septal GABAergic system in temporal lobe epilepsy, which is a possible cause of a sharp change in the oscillatory properties of septal neurons. These findings contribute to elucidation of the mechanisms of temporal lobe epilepsy.

[Mucous discharge features at duodenal ulcer in adolescents].

In recent years, significant progress in studying the pathogenesis, diagnosis and treatment of gastrointestinal diseases in children and adolescents. However, steady growth of erosive and ulcerative lesions of the mucous membrane of the stomach and duodenum in adolescents. A major role in the formation of chronic diseases of the stomach and duodenum assigned lower sacrificial function of the mucous membrane, which has multifactorial protection against pathogenic effects. The results of the study lektingistohimicheskogo mucus formation in the duodenal ulcer in adolescents.

[Coherence and phase analysis of theta-oscillations in the septohippocampal system during seizures].

Interrelations of the hippocampus and medial septal area (MSA) in the theta band (4-8 Hz) were studied during seizures produced by electrical kindling in waking guinea pigs. Field activity (EEG) was analyzed using the wavelet transform. A decrease in coherence of theta-oscillations in the hippocampus and MSA was observed during seizures. Phase analysis showed that in the beginning of kindling the MSA led in phase, but after formation of the pathological focus, MSA lagged the hippocampus. The data may contribute to understanding mechanisms of temporal lobe epilepsy.

[Receptor caspase-dependent and caspase-independent ways of human lymphocytes apoptosis induced by UV-radiation].

Changes of a structural condition of DNA, expression level of the membrane Fas-receptor and of functional activity of the human lymphocytes caspase-3 in dynamics of apoptosis development, induced by influence of UV-light (240-390 nm) in doses 151, 1510 and 3020 J/m2 are studied. It is established, that the UV-irradiation of lymphocytes in doses of 151 and 1510 J/m2 causes realisation of receptor caspase-dependent apoptosis way. The assumption about switching possibility of receptor caspase-dependent way apoptosis on receptor caspase-independent way at increase of a dose of UV-light to 3020 J/m2 is made.

[Relationships between the hippocampus and medial septal region and their alterations during epileptogenesis].

EEGs of the hippocampus and the medial septal region (MSR) in the control conditions and during repeated stimulation of the perforant path were simultaneously recorded in awake guinea pigs. Changes in correlation of activity of these structures during seizures provoked by the stimulation (model of acute epilepsy) and in the process of epileptogenesis induced by the kindling (model of chronic epilepsy) were analyzed. A high correlation of the baseline activities of the hippocampus and MSR observed in the control sharply decreased during acute and chronic seizures. Kindling led to emergence of the MSR capability of hippocampus-independent generation of the field seizure discharges. In the process of kindling the progressive disintegration of activities of the hippocampus and the MSR was revealed being indicative of disorders in functioning of the septohippocampal network during epileptogenesis.

[Alterations in the oscillatory activity of neurons in the medial septal area in animals with the chronic temporal lobe epilepsy model].

Comparative investigation of activity of medial septal neurons, which was performed by extracellular recordings in the brain slices of health guinea pigs and in such ones of animals with the model of chronic temporal lobe epilepsy, revealed the distinctions between them, concerning a neuronal frequency and pattern of discharges. In the epileptic brain, twofold increase of general level of activity was observed comparative to control one, owing to augmentation of frequency of discharges in non-regular and regular non-bursting neurons. Sharp increase (three times as much) of number of cells with rhythmic burst discharges and changing of parameters of burst activity were discovered, the letter both in the neurons with endogenous (pacemaker) pattern and in the cells secondary involved in the rhythmic activity. Possible mechanisms of these alterations are discussed. The present data advance the understanding of the processes of a pathological synchronization and may promote creation of new approaches to treatment this disease.

[Hexamethonium, nicotinic receptor blocker, changes the neuronal reactions on glutamate in the medial septal area in vitro].

Despite the great interest in studying the medial septal area, the interactions of its neurochemical systems are not yet clearly understood. The aim of this study was to elucidate the role of nicotinic receptors in the interaction of glutamatergic and cholinergic systems of the medial septal area. The effect of L-glutamate (1 microM) on septal neurons was studied under the application of hexamethonium, nicotinic cholinoreceptor blocker by using the method of extracellular recording of neuronal activity in brain slices of ground squirrels. The response of septal neurons to glutamate depended on the type of their initial activity and on the presence of pacemaker properties. For the first time, the ability of septal neurons to respond to glutamate with an increase in burst frequency was shown. The influence of hexamethonium on the neuronal activity was similar to that of glutamate. After a preliminary application of hexamethonium, the reactions of neurons to glutamate changed. The excitatory reactions were masked, while the inhibitory reactions became stronger. It was found that nicotinic cholinergic receptors modulated the reactions of MS-DB cells to glutamate and the expression of the oscillatory properties of the septal neuronal network.

[The role of medial septal area in the neural control over hibernation]. / Rol' medial'noi septal'noi oblasti v nervnom kontrole gibernatsii.

Hibernation (winter sleep) is a kind of unique adaptive behavior of small mammals subjected to fine and complex central control. One of the most promising approaches to this problem is a search for the mechanisms providing brain control under conditions of a sharp decrease in temperature, (virtually, to zero) and metabolic rate. Studies conducted at the Laboratory of System Organization of Neurons under the supervision of Professor O.S. Vinogradova confirmed the hypothesis of the special role of the septohippocampal system in the control of winter sleep. Together with a brief characterization of hibernation in general, the data obtained at the Laboratory are also summarized in the review. The experimental evidence for the role of the medial septal area as a "sentry post" in hibernation is presented.

[Modulation of neuronal evoked responses to the medial septal area of hibernating ground squirrels by some neuropeptides after chronic disconnection between septum and preoptico-hypothalamic structures]. / Moduliatsiia neiropeptidami vyzvannykh otvetov neironov medial'noi septal'noi oblasti giberniruiushchikh suslikov v usloviiakh ee khronicheskoi izoliatsii ot preoptiko-gipotalamicheskikh struktur.

Effects of some neuropeptides identified in the brain of hibernators (TSKYR, TSKY, DY) and of monoaminergic neurotransmitters (noradrenaline and serotonin) on responses of the medial septal neurons evoked by intraseptal electrical stimulation were analyzed in slices taken from the ground squirrels with chronic basal undercutting of the septum. Despite the elimination of direct contacts with the preoptic area and afferents ascending in the medial forebrain bundle, the neurons retained almost normal level of reactivity and distribution of the reaction types. The neuropeptides effectively modulated neuronal responses of various types, including oligosynaptic short-latency single-spike responses. The latter were strongly facilitated by the neuropeptides. As a rule, changes in the responses to electrical stimulation were independent of the spontaneous activity shifts (in 78% of the tests). It was suggested that the neuropeptides exert a double influence on the septal neurons: direct nonsynaptic effects on the pacemaker potential responsible for the background activity and modulation of synaptic processes. Our experiments showed that descending influences of the septo-hippocampal system are not crucial for the entrance into the hibernation state and its tonic maintenance. The influences of the thermoregulatory--circadian structures of the preoptico-hypothalamic area determine the paradoxically increased latent excitability of septal neurons that allows the septo-hippocampal system to gate external stimuli and organize arousal of the forebrain during hibernation in case of emergency.

[Effect of chronic disconnection between septal area and hypothalamus on modulation of background activity of septal neurons by biologically-active substances in hibernators]. / Vliianie khronicheskogo raz''edineniia septal'noi oblasti i gipotalamusa na moduliatsiiu fonovoi aktivnosti septal'nykh neironov biologicheski aktivnymi veshchestvami u gibernantov.

Our previous work demonstrated paradoxically increased excitability of the medial septal (MS) neurons during hibernation of ground squirrels in comparison to waking animals. Recently this was supported by demonstration of higher efficacy of the neuropeptides identified in the brain of hibernators in septal slices of hibernating animals. To decide whether this increased excitability is determined by endogenous properties of the pacemaker septal neurons, or it depends on the influences of thermoregulatory-circadian mechanisms of preoptico-hypothalamic area, testing of the neuropeptides (TSKYR, TSKY, DY) and neurotransmitters participating in control of hibernation (serotonin and noradrenaline) was repeated on septal slices taken from the brain of hibernating animals two weeks after operation disconnecting it from the hypothalamus. Effects of neuropeptides in the deafferented hibernating animals neither quantitatively (low reactivity level), nor qualitatively (distribution of inhibitory and excitatory responses) differed from the data obtained in waking animals. Decrease of reactivity occurred at the expense of the neurons with regular pacemaker-like spontaneous activity. Thus, increased reactivity of the MS neurons to neuropeptides in hibernating animals depends mainly on influence of the hypothalamic centres controlling hibernation behavior upon pacemaker neurons of the MS. Contrary to the neuropeptides, serotonin and noradrenaline were highly effective in deafferented septum. They evoked stronger changes of background activity (shorter latencies and more rapid development of maximal shifts), presumably as a result of development of denervation hypersensitivity after deafferentation.

[The role of humoral factors in the pathogenesis of the postoperative syndrome]. / Rol' nekotorykh gumoral'nykh faktorov v patogeneze posleoperatsionnogo bolevogo sindroma.

Blood plasma levels of "pain substances" (serotonin, histamine, prostaglandin F2 alpha, adrenaline /A/) and neuropeptides (beta-endorphin, somatostatin) have been evaluated in 39 patients during the early postoperative period after lung and mediastinum surgery. The studies have shown that the content of these biologically active substances increases considerably. Following stellate ganglion blockade A concentration decreased significantly, the uptake of narcotic analgesics used for postoperative analgesia reduced 1.7-fold, however the levels of "pain substances" and neuropeptides remained unchanged. It is believed that postoperative pain syndrome develops due to the elevation of the levels of the substances under study. Stellate ganglion blockade produces only sympatholytic effect, which shows the necessity of the elaboration of drug therapeutic techniques blocking "pain" receptors and using "pain substance" antagonists.

[Psychoautonomic syndrome in occlusive lesions of the basin of the internal carotid artery]. / Psikhovegetativnyi sindrom pri okkliuzionnykh porazheniiakh v basseine vnutrennei sonnoi arterii.

A complex psychophysiological investigation of 120 patients with occlusions in the territory of the internal carotid artery revealed regular disorders of the suprasegmental vegetative structures manifested as psychovegetative syndrome. This may be considered as one of the cases of limitation of the adaptation capacities of the of these patients.