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1.
Bioresour Technol ; 99(14): 6365-71, 2008 Sep.
Article in English | MEDLINE | ID: mdl-18226891

ABSTRACT

Occurrence of mold was visually monitored for 26days on samples of major anatomical components of corn stover maintained at several storage temperatures (T) and water activities (a(w)). Glass desiccators with saturated salt solutions placed in temperature controlled chambers provided simulated storage conditions with temperatures ranging from 10 degrees C to 40 degrees C and water activities ranging from 0.11 to 0.98. Mold affected leaf, stalk skin, and stalk pith equally at water activity greater than 0.9. As expected, a combination of increased water activity greater than 0.9 and temperatures greater than 30 degrees C was conducive to mold growth. Based on material moisture content during the initial mold growth, it was postulated that among the corn stover components the stalk pith was the least resistant to mold growth followed by stalk skin and leaf for the studied range of temperature and water activity. Mold growth models fitted well with the observation. A linear mold-free days predictions using a three-parameter regression model (T, a(w), and T x a(w)) was superior (R(2)=0.99) to other models considered. The exponential spoilage model using two parameter T and a(w) also gave comparable performance (R(2)=0.95). Among the independent factors, T x a(w) product was the most significant (p=0.0069) followed by T (p=0.0114), and a(w) (p=0.3140) in explaining the experimental data. The developed models can be applied to predict the safe storage period of corn stover components exposed to various temperature and moisture environmental conditions.


Subject(s)
Fungi , Models, Theoretical , Water/chemistry , Zea mays/microbiology , Fungi/growth & development , Zea mays/chemistry
2.
J Anim Sci ; 82(9): 2734-41, 2004 Sep.
Article in English | MEDLINE | ID: mdl-15446490

ABSTRACT

This research assessed the potential of increasing PUFA concentrations and the effect on flavor volatiles in red meat by feeding ground, full-fat soybean supplemented in casein complex. Supplements consisted of untreated ground, full-fat soybean (CO) or ground, full-fat soybeans treated with acetaldehyde (AC) or diacetyl (DA) to form gels. On a DM basis, the control (CO), AC, and DA supplements contained 48.6, 50.0, and 49.1% CP and 17.3, 17.3, and 17.4% fat, respectively. Weaned feeder lambs (n = 18) were divided into three treatment groups with two pens of three lambs per group. One of three supplements (200 g of DM) plus 1 kg DM of a ground corn basal diet and 0.36 kg DM of grass hay was fed daily to each of six lambs in a group for 9 wk. Samples of the intramuscular (LM), intermuscular, subcutaneous, and kidney fat were obtained from each lamb carcass for determination of total lipid contents and fatty acid profiles. Flavor volatiles of broiled LM were also analyzed. Total fat content of the LM was 3.7, 4.6, and 2.6% for lambs consuming diets supplemented with CO, AC, and DA, respectively. Compared with lambs fed the untreated supplement (CO), lambs supplemented with AC or DA had 1) higher (P < 0.05) concentrations of linoleic (4.80 vs. 6.37 or 6.80%) and linolenic (0.28 vs. 0.43 or 0.45%) acids in the LM nonpolar lipids; 2) a higher (P < 0.05) concentration of linoleic acid (22.1 vs. 27.1 or 25.6%), but a lower (P < 0.05) concentration of oleic acid (17.2 vs. 13.0 or 13.1%), in the LM polar lipids; 3) a higher (P < 0.05) concentration of linoleic acid (3.77 vs. 6.13 or 6.06%) in subcutaneous fat; and 4) higher (P < 0.05) concentrations of linoleic (4.46 vs. 7.65 or 7.13%), linolenic (0.50 vs. 0.85 or 0.80%), and stearic (24.9 vs. 27.2 or 26.9%) acids, but a lower (P < 0.05) concentration of oleic acid (39.1 vs. 35.4 or 36.3%), in kidney fat. In broiled LM chops, 21 volatiles were identified, including seven alkanals, seven 2-alkenals, two 2,4-alkadienals, and five other compounds, but most differences in the volatile concentrations among lambs fed the different supplements did not correspond to concentration differences in their precursor fatty acids. Results indicated that compared with the untreated supplement (CO), AC and DA supplements protected linoleic (C 18:2n6) and linolenic (C18:3n3) acids in soybean oil from degradation in the rumen of the lambs, resulting in increased deposition in the muscle and adipose tissues of lamb.


Subject(s)
Body Composition/drug effects , Fatty Acids, Unsaturated/analysis , Glycine max , Meat/standards , Sheep/metabolism , Acetaldehyde/chemistry , Animals , Body Composition/physiology , Diacetyl/chemistry , Dietary Supplements , Fatty Acids, Unsaturated/administration & dosage , Fatty Acids, Unsaturated/metabolism , Gels , Linoleic Acid/analysis , Meat/analysis , Random Allocation , Taste , Volatilization , alpha-Linolenic Acid/analysis
3.
J Food Prot ; 65(1): 222-37, 2002 Jan.
Article in English | MEDLINE | ID: mdl-11808801

ABSTRACT

Recently, much attention has been focused on the safety of fruits and vegetables. Washing is a fundamental operation in the processing of produce. Aqueous spray energy can be, and often is, used to remove mineral, chemical, or biological contaminants from produce. A few advantages of spray washing over washing by dipping, soaking, or gravity rinse are increased energy directed to contaminants, reduced volume of water use and wastewater generation, and reduced water uptake by produce. The kinetic energy of the spray droplets produces the cleaning action. Increased spray pressure increases energy. If the energy is too great, produce may be physically damaged. If the energy is too little, the surface may not be cleaned. Indeed, studies on meat have shown that water pressures ranging from 1,379 to 2,070 kPa (200 to 300 psi) are effective in reducing microbial contamination, and a water flow rate of 7.5 liters/min is recommended. Water temperature >70 degrees C has been found to reduce bacterial counts in carcass tissue by 2 to 3 log CFU/cm2. These levels are likely too high for the fragile produce; hence, the main function of spray washing in produce applications will probably shift to being a delivery system for antimicrobial agents. Several other equipment, process, and product variables are relevant to the optimization of such a system. Qualities of the spray, such as droplet spectrum, droplet velocity, angle of droplet impingement, number and orientation of nozzles, spray rate, and resident time of the produce in the sprayer, also can be manipulated to adjust the amount of energy directed to the surface. There is a need to scientifically investigate the effects of these processes and equipment parameters on the removal of microbiological contaminants on meats and produce. Such empirical investigations guided by the results from fundamental studies about produce surface characteristics and the mechanism of bacterial attachment to plant tissue surfaces would allow for the efficient development of spray washers that effectively decontaminate produce.


Subject(s)
Bacteria/growth & development , Fruit/microbiology , Meat/microbiology , Sanitation/methods , Vegetables/microbiology , Animals , Bacterial Adhesion , Colony Count, Microbial , Food Contamination/prevention & control , Food Microbiology , Pressure , Temperature
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