ABSTRACT
The etiology and pathogenesis of polycystic ovary syndrome (PCOS) is still unknown. Using real-time PCR, we detected that polycystic ovaries showed almost ten times lower expression of ghrelin mRNA than normal ovaries, whereas the mRNA levels in blood cells were similar in both study groups. This suggests that the presence of ghrelin in PCOS and normal ovaries may have an autocrine/paracrine modulatory effect on ovary functions and local significance in the etiology of PCOS.
Subject(s)
Polycystic Ovary Syndrome/metabolism , Receptors, G-Protein-Coupled/metabolism , Adult , Case-Control Studies , Female , Humans , RNA, Messenger/analysis , Receptors, Ghrelin , Reverse Transcriptase Polymerase Chain ReactionABSTRACT
Genital HPV16 has tropism to cervical epithelial tissue which is highly steroid hormone dependent. Protein/DNA interaction with the fragment of viral DNA containing the glucocorticoid-response element (GRE/PRE) did not indicate the proteins which can bind to the GRE element in normal epithelial cervical tissue. NFI is one of the cellular factors which can participate in the transcription of viral genes in study tissue.
Subject(s)
Cervix Uteri/physiology , DNA, Viral/metabolism , Papillomaviridae/genetics , Blotting, Southern , Blotting, Western , DNA-Binding Proteins/metabolism , Electrophoresis, Polyacrylamide Gel , Epithelium/physiology , Female , Humans , Protein Binding , Receptors, Estrogen/metabolism , Receptors, Progesterone/metabolism , Response Elements/physiologySubject(s)
Condylomata Acuminata/genetics , DNA, Viral/genetics , Papillomaviridae/genetics , Base Sequence , Female , Humans , MaleABSTRACT
Using nucleic acid hybridization and polymerase chain reaction (PCR) HPV-DNA sequence was detected in endometrial and ovarian carcinomas. This sequence was amplified with primer specific for E1 region of DNA-HPV 18 and hybridized with DNA-HPV 18. The presence of HPV-DNA in cancer studies suggests that human papillomavirus can also be involved in carcinogenesis of the upper genital tract of women.
Subject(s)
DNA, Viral/analysis , Endometrial Neoplasms/virology , Ovarian Neoplasms/virology , Papillomaviridae/genetics , Adult , Aged , Female , Humans , Middle Aged , Nucleic Acid Hybridization , Polymerase Chain ReactionABSTRACT
There are few publication in the literature on DNA HPV 16 in the lymph nodes of cervical cancer patients. Presence of HPV 16 in the lymph nodes is correlated with metastasis. Our investigation suggests that HPV 16 may be located in the lymph nodes without the presence of cancer cells.
