ABSTRACT
Peripheral blood and bone marrow morphology, blood and bone marrow lymphocyte subpopulation composition were studied in two children with cyclic neutropenia, using flow cytofluorometry, monoclonal antibodies, colony-forming capacity of granulocytic macrophagal precursors in semi-fluid agar. The studies were conducted in varying periods of the neutropenic cycle. Differences were revealed in immunohematologic parameters and clinical course of neutropenia in the two patients. The analysis of the literature data and the authors' own observations of the patients with cyclic neutropenia permitted a suggestion on high heterogeneity of pathogenetic mechanisms of this disease.
Subject(s)
Bone Marrow/pathology , Hematopoietic Stem Cells/pathology , Neutropenia/diagnosis , Neutrophils/pathology , Adolescent , Child , Colony-Forming Units Assay/methods , Female , Flow Cytometry/methods , Humans , Leukocyte Count , Male , Neutropenia/blood , Neutropenia/etiology , Neutropenia/pathology , Periodicity , Recurrence , Syndrome , Time FactorsSubject(s)
Dysgammaglobulinemia/diagnosis , IgA Deficiency , Immunoglobulin G/analysis , Immunoglobulin M/analysis , Adolescent , Child , Child, Preschool , Humans , Infant , Population Surveillance , RiskABSTRACT
The capacity of leukocytes from children with primary immunodeficiency to produce alpha- and gamma-interferons in vitro was studied. Interferon response of leukocytes in most of the patients examined was found to be practically unchanged. The immunostimulating therapy in some cases exerted a regulating effect on leukocyte capacity for interferon production. It is assumed that the interferon-producing function of T lymphocytes may be preserved in patients suffering from primary immunodeficiency.
Subject(s)
Immunologic Deficiency Syndromes/immunology , Interferon Type I/biosynthesis , Adolescent , Cells, Cultured , Child , Child, Preschool , Encephalomyocarditis virus , Humans , Immunologic Deficiency Syndromes/therapy , Immunotherapy , Interferon-gamma/biosynthesis , Male , Newcastle disease virus , T-Lymphocytes/immunologyABSTRACT
The immunochemical study of the blood sera of children with chronic and relapsing infections has shown an increase in the content of alpha 2-macroglobulin and alpha 1-antitrypsin in the absence of significant changes in the concentration of immunoglobulins and complement components. The immunochemical analysis of the system of soluble leukocytic antigens (SLA) has revealed a decrease in the level of SLA-1 simultaneously with the presence of redundant amounts of SLA-5 and SLA-8.
Subject(s)
Antigens/analysis , Autoantigens/analysis , Blood Proteins/analysis , Furunculosis/immunology , Leukocytes/immunology , Otitis Media/immunology , Respiratory Tract Infections/immunology , Child , Child, Preschool , Chronic Disease , Humans , Immunodiffusion , Recurrence , SolubilitySubject(s)
Hypergammaglobulinemia/diagnosis , Immunoglobulin E/analysis , Immunologic Deficiency Syndromes/diagnosis , Adolescent , Chemotaxis, Leukocyte/drug effects , Child , Child, Preschool , Chronic Disease , Female , Humans , Hypergammaglobulinemia/immunology , Hypersensitivity, Delayed/immunology , Immunologic Deficiency Syndromes/immunology , Lymphocytes/drug effects , Lymphocytes/immunology , Male , Rosette Formation , Staphylococcal Infections/diagnosis , Staphylococcal Infections/immunology , Theophylline/pharmacologyABSTRACT
The following three patterns of cell surface of lymphoid elements were identified in the course of rastr electron microscopic studies carried out in children with lymphosarcoma: marked plicate , relatively smooth and tuberous. No relationship between the pattern of tumor cell membrane and the site of sampling was established. Such diversity of lymphoid element surface pattern in children with lymphosarcoma is suggestive of the varying level of tumor cell differentiation and is, possibly, a manifestation of different stages of lymphocyte development.
