Identification of a multi-modal mechanism for Se(VI) reduction and Se(0) allotropic transition by Stenotrophomonas bentonitica.

Microorganisms can play a key role in selenium (Se) bioremediation and the fabrication of Se-based nanomaterials by reducing toxic forms (Se(VI) and Se(IV)) into Se(0). In recent years, omics have become a useful tool in understanding the metabolic pathways involved in the reduction process. This paper aims to elucidate the specific molecular mechanisms involved in Se(VI) reduction by the bacterium Stenotrophomonas bentonitica. Both cytoplasmic and membrane fractions were able to reduce Se(VI) to Se(0) nanoparticles (NPs) with different morphologies (nanospheres and nanorods) and allotropes (amorphous, monoclinic, and trigonal). Proteomic analyses indicated an adaptive response against Se(VI) through the alteration of several metabolic pathways including those related to energy acquisition, synthesis of proteins and nucleic acids, and transport systems. Whilst the thioredoxin system and the Painter reactions were identified to play a crucial role in Se reduction, flagellin may also be involved in the allotropic transformation of Se. These findings suggest a multi-modal reduction mechanism is involved, providing new insights for developing novel strategies in bioremediation and nanoparticle synthesis for the recovery of critical materials within the concept of circular economy.

Biostimulation of indigenous microbes for uranium bioremediation in former U mine water: multidisciplinary approach assessment.

Characterizing uranium (U) mine water is necessary to understand and design an effective bioremediation strategy. In this study, water samples from two former U-mines in East Germany were analysed. The U and sulphate (SO42-) concentrations of Schlema-Alberoda mine water (U: 1 mg/L; SO42-: 335 mg/L) were 2 and 3 order of magnitude higher than those of the Pöhla sample (U: 0.01 mg/L; SO42-: 0.5 mg/L). U and SO42- seemed to influence the microbial diversity of the two water samples. Microbial diversity analysis identified U(VI)-reducing bacteria (e.g. Desulfurivibrio) and wood-degrading fungi (e.g. Cadophora) providing as electron donors for the growth of U-reducers. U-bioreduction experiments were performed to screen electron donors (glycerol, vanillic acid, and gluconic acid) for Schlema-Alberoda U-mine water bioremediation purpose. Thermodynamic speciation calculations show that under experimental conditions, U(VI) is not coordinated to the amended electron donors. Glycerol was the best-studied electron donor as it effectively removed 99% of soluble U, 95% of Fe, and 58% of SO42- from the mine water, probably by biostimulation of indigenous microbes. Vanillic acid removed 90% of U, and no U removal occurred using gluconic acid.

Beneficial Shifts in the Gut Bacterial Community of Gilthead Seabream (Sparus aurata) Juveniles Supplemented with Allium-Derived Compound Propyl Propane Thiosulfonate (PTSO).

This study analyzes the potential use of an Allium-derived compound, propyl propane thiosulfonate (PTSO), as a functional feed additive in aquaculture. Gilthead seabream (Sparus aurata) juveniles had their diet supplemented with this Allium-derived compound (150 mg/kg of PTSO) and were compared with control fish. The effects of this organosulfur compound were tested by measuring the body weight and analyzing the gut microbiota after 12 weeks. The relative abundance of potentially pathogenic Vibrio and Pseudomonas in the foregut and hindgut of supplemented fish significantly decreased, while potentially beneficial Lactobacillus increased compared to in the control fish. Shannon's alpha diversity index significantly increased in both gut regions of fish fed with a PTSO-supplemented diet. Regarding beta diversity, significant differences between treatments only appeared in the hindgut when minority ASVs were taken into account. No differences occurred in body weight during the experiment. These results indicate that supplementing the diet with Allium-derived PTSO produced beneficial changes in the intestinal microbiota while maintaining the productive parameters of gilthead seabream juveniles.

Encuesta perinatal de la Sociedad Gallega de Obstetricia y Ginecología 2010-2011 / Perinatal survey of the Galician Society of Obstetrics and Gynecology 2010-2011

Objetivo. Obtener y evaluar los datos de la asistencia perinatal en Galicia. Material y métodos. Estudio descriptivo, retrospectivo y multicéntrico. Se solicitó la colaboración de todos los centros sanitarios gallegos con asistencia al parto para la realización de la base de datos perinatales de los años 2010 y 2011. Resultados. Realizamos el estudio estadístico epidemiológico de 29.254 nacimientos acontecidos en nuestra comunidad en el bienio 2010-2011, lo cual representa el 67,2% del total de nacimientos de Galicia en ese periodo. Analizamos las siguientes variables: edad materna, estática fetal, amenorrea en el momento del parto, tipo de parto, indicaciones de cesáreas, y mortalidad perinatal y materna (AU)

Objective. To obtain and evaluate information on perinatal care in Galicia (Spain). Material and methods. We performed a descriptive, retrospective, multicenter study. All centers in Galicia involved in deliveries were requested to collaborate in the perinatal database for 2010 and 2011. Results. We performed a statistical, epidemiological study of 29,254 births in our region in the 2-year period from 2010 to 2011, representing 67.2% of all births in Galicia in this period. The following variables were analyzed: maternal age, fetal statics, amenorrhea at delivery, type of delivery, indications for cesarean section, and perinatal and maternal mortality (AU)

Cutaneous lasers.

There has been a remarkable development and evolution of laser technology, leading to adaptation of lasers for medical use and the treatment of skin problems and disorders. Many treatments that required incisional surgery and other invasive methods are now preferentially treated with a laser. Although laser advances have resulted in the availability of some amazing tools, they require the clinical skill and judgment of the clinician for their optimal use. This article provides a clinically oriented overview of many of the lasers valuable in facial plastic surgery. Basic science, clinical adaptations, and patient management topics are covered.

Carcinoma indiferenciado de trompa / Undifferentiated carcinoma of the fallopian tube

La neoplasia primaria de trompa de Falopio es una entidad poco frecuente. El diagnóstico preoperatorio es difícil y en muchas ocasiones es un hallazgo intraoperatorio o un diagnóstico histopatológico. Presentamos un caso de cáncer primario de trompa en una mujer de 61 años con algias pélvicas de un mes de evolución, masa pélvica izquierda dolorosa y Ca 125 elevado. Las pruebas diagnósticas fueron compatibles con patología uterina maligna (sarcoma uterino) o patología neoplásica ovárica izquierda con infiltración uterina como segunda posibilidad. El resultado histológico fue de carcinoma indiferenciado de trompa izquierda que infiltra pared uterina en cuerpo y cérvix. Actualmente, la paciente se encuentra sometida a tratamiento quimioterápico (AU)

Primary carcinoma of the fallopian tube is a rare entity. Preoperative diagnosis is difficult and these tumors are usually detected during surgery or on histopathological examination. We describe the case of a 61-year-old woman with pelvic pain, left adnexal mass and elevated Ca125 levels. Diagnostic tests were compatible with uterine sarcoma or primary left ovarian carcinoma with uterine invasion. Histopathological analysis showed a primary undifferentiated carcinoma of the left fallopian tube involving the uterus and cervix. The patient is currently undergoing chemotherapy (AU)

Cerclaje cervical de emergencia: nuestra experiencia / Emergency cervical cerclage: our experience

Objetivo: Analizar la efectividad del procedimiento a través de los resultados perinatales obtenidos, mediante un estudio descriptivo y retrospectivo. Material y métodos: Se han revisado los casos de cerclaje cervical de emergencia aplicados en nuestro servicio entre los años 2000 y 2007, ambos incluidos, estableciendo criterios de selección que determinan a las pacientes susceptibles beneficiarse de este método. Resultados: Hemos obtenido una supervivencia neonatal libre de secuelas en el 87,5 % de los casos. Las complicaciones asociadas fueron menores, entre las que destacan la amenaza de parto prematuro y la rotura prematura pretérmino de membranas. Conclusión: Pese a la escasa bibliografía acerca de este tipo de cerclaje y sus resultados, a veces contradictorios, en nuestro centro optamos por éste como una alternativa eficaz a la actitud expectante en los casos de incompetencia cervical tardía como los descritos (AU)

Objective: To analyze the effectiveness of emergency cervical cerclage through a descriptive and retrospective analysis of our perinatal results.
Material and method: We reviewed cases in which emergency cervical cerclage was applied in our department between 2000 and 2007, both inclusive. During this review we established the necessary criteria to select the female patients likely to benefit from this method. Results: In the patients studied, we achieved a sequel-free neonatal survival rate of 87.5%. The associated complications were minor, mainly threatened preterm labor and preterm premature rupture of membranes. Conclusion: Despite the scarce literature about emergency cervical cerclage and its results (which are sometimes contradictory), we have chosen this technique as an effective alternative to expectant management in cases of late cervical incompetence, such as those described above (AU)

Hallazgos ecográficos extragenitales en la urgencia ginecológica / Extragenital ultrasonographic findings in gynecological emergencies

Presentamos varios resúmenes de casos clínicos, con la iconografía correspondiente, de pacientes vistas en la urgencia de ginecología del hospital. El nexo de unión de las pacientes es que, aunque su motivo de consulta o de interconsulta era ginecológico, los hallazgos de la ecografía de urgencia orientaron los casos a procesos extragenitales, más o menos graves, dentro del área abdominal. Se pretende remarcar la necesidad que tiene el ginecólogo de conocer los principales procesos abdominales con reflejo ecográfico para encauzar clínicamente de modo correcto a sus pacientes y realizar adecuadamente los diagnósticos diferenciales

We summarize several cases of patients attended by a hospital emergency gynecology service and present the corresponding images. Although the reason for consultation or interconsultation was gynecological, the common factor among these patients was that the results of emergency ultrasonography indicated extragenital processes, of greater or lesser severity, within the abdomen. We highlight the need for gynecologists to be familiar with the main abdominal processes captured by ultrasonography to allow correct patient management and differential diagnoses

Extracción de DIU mediante histeroscopia en la gestación precoz: nuestra experiencia en 4 casos / Intrauterine device extraction through hysteroscopy in early pregnancy. Our experience in 4 cases

Objetivos: Valoración de la eficacia de la histeroscopia en la extracción controlada de DIU en la gestación precoz. Sujetos y métodos: Seguimiento clínico de la evolución de 4 gestaciones en las que se extrae el DIU, mediante histeroscopia, antes de la semana 10, en el período 2003-2005. Resultados: Tres de las gestaciones evolucionaron correctamente y sin complicaciones tras la extracción del DIU; no se objetivaron anomalías que pudieran deberse a la histeroscopia. En un caso se produjo un aborto a las 15 semanas por rotura de membranas. Conclusiones: La aplicación de la histeroscopia en el embarazo ha sido siempre limitada. Debemos valorar en todos los casos la posible lesión del nervio óptico del feto por lo que se desaconseja la práctica de esta técnica en gestantes de más de 10 semanas. Una vez retirado el DIU, en la mayoría de los casos el embarazo prosigue sin complicaciones posteriores

Objectives: To evaluate the utility and effectiveness of hysteroscopic retrieval of intrauterine devices (IUDs) in early pregnancy. Subjects and methods: We followed-up four pregnant women with IUDs with retracted tails removed by hysteroscopy before 10 weeks of pregnancy from 2003 to 2005. Results: After hysteroscopy, pregnancy course was successful in three women, without complications that could be attributed to the procedure. There was one abortion due to rupture of fetal membranes at 15 weeks' gestation. Conclusions: The application of hysteroscopy in pregnancy has always been limited. In all patients, a possible optic nerve lesion in the fetus should be evaluated. This technique should not be performed after the 10th week of pregnancy. In most patients, pregnancy continues without complications after IDU removal

Efeito da tela de politetrafluoroetileno expandido colada no espaço pré-peritonial por videolaparoscopia em suínos / Effects pf espanded polytetrafluorethylenein the prepitoneal space introduced by videolaparoscopy in pigs

Com o objetivo de observar os efeitos produzidos pela tela de politetrafluoroetileno expandido (PTEE-e), no espaço pré-peritoneal, foram utilizados 20 suínos (n = 20) da raça Large White, todos machos, distribuídos em dois grupos de dez animais. A técnica operatória utilizada foi a videolaparoscopia transabdominal. De um lado, na região inguinal, o espaço pré-peritoneal foi dissecado e a tela fixada; na região contralateral, o procedimento foi o mesmo, sem a colocação da tela, servindo como controle. O peritônio parietal local foi aproximado com uma sutura contínua de poliglactina 910. A eutanásia foi realizada aos sete e vinte dias de pós-operatório. No primeiro grupo, um animal apresentou aderência no local de implantação da tela e, no segundo grupo, um animal também apresentou aderência, mas no lado controle. Concluiu-se, baseado na presente pesquisa, que a tela de PTEE-e não provocou a formação de aderências, quando colocada no espaço pré-peritoneal em suínos.