ABSTRACT
We have studied the effect of domestic derivatives of 3-hydroxypyridine and succinic acid (emoxipine, reamberin, and mexidol) on proliferation of E. coli, S. aureus and E. faecalis in discontinuous cultures (DCs) over a period of 1 or 24 h. It is established that the drugs studied exhibit a biphase effect on the number of bacterial colony-forming units (CFUs) in DCs of E. coli and E.faecalis. This effect is manifested by the initial 1.3 - 3.5-fold decrease in the number of CFUs (after 1-h incubation with drugs) with subsequent return to the control level or 1.8 - 8.8-fold increase over the control level (after 24-h incubation with drugs). In DCs of S. aureus that were incubated with the drugs, we observed 1.1 - 1.95-fold increase in CFU number after I-h incubation and 1.8 - 2.4-fold increase after 24-h incubation. On the whole, the results of our studies demonstrated a positive effect of 3-hydroxypyridine and succinic acid derivatives on bacterial growth in DC. These drugs stimulated increase in CFU number in DCs of test bacterial strains even at concentrations as low as 10â»9 -10â»6. The well-known efficiency of 3-hydroxypyridine and succinic acid derivatives in treatment of infectious pathology appears independent of their direct effect on bacterial growth.
Subject(s)
Enterococcus faecalis/drug effects , Escherichia coli/drug effects , Meglumine/analogs & derivatives , Picolines/pharmacology , Staphylococcus aureus/drug effects , Succinates/pharmacology , Antioxidants/pharmacology , Colony Count, Microbial , Enterococcus faecalis/growth & development , Escherichia coli/growth & development , Hormesis , Meglumine/pharmacology , Staphylococcus aureus/growth & developmentABSTRACT
Express-test by the method of coherent fluctuation nephelometry for urine contamination was carried out on two prototype instruments with standard polystyrene photometric cuvettes. We analyzed 209 and 119 urine samples. Due to high sensitivity of the method, up to 50% negative samples were detected within 10 min by initial opacity and 90% negative samples were detected during 3.5 h by registration of the bacterial growth curves.
Subject(s)
Bacteriuria/diagnosis , Nephelometry and Turbidimetry/methods , Urinalysis/methods , HumansABSTRACT
Experimental data are considered, suggesting existence of factors specifically regulating division of hepatocytes. The factors, found in regenerating liver tissue and its blood serum of hepatectomized animals, stimulated the cell division. The substance from intact liver cells or liver chalones inhibited the division of hepatocytes.
Subject(s)
Cell Division , Liver/physiology , Animals , Growth Inhibitors/physiology , Kinetics , Liver RegenerationABSTRACT
Activity of G1 and G2 chalones was found in liver tissue extract. The chalone G1 as compared with the chalone G2 was responsible for longer effect on mitosis of regenerating liver cells. The influence of the chalones was reversible. DNA synthesis was inhibited similarly after treatment of the animals with 1.0, 0.5 or 0.25 mg of the liver tissue extract per 1.0 g of the body mass.
Subject(s)
DNA/biosynthesis , Growth Inhibitors/physiology , Liver Regeneration , Liver/metabolism , Mitosis , Animals , DNA Replication , Kinetics , Male , RatsABSTRACT
An attempt was made to isolate the liver tissue chalone blocking the transition of hepatocytes from G2 period of the cell cycle into mitosis/g2-chalone/. The purification was carried out using gel filtration through Sephadex G-75 and hydroxyapatite chromatography as well as by means of ultrafiltration through Diaflo membranes XM-300, XM-100 and PM-10. The biologically active preparation, obtained by these two procedures, was purified 100-fold. Molecular mass of G2 chalone was apparently less then 10,000; it penetrated through PM-10 membrane. The chalone appears to form complexes with high molecular carriers.
Subject(s)
Growth Inhibitors/isolation & purification , Liver/analysis , Animals , Chalones , Chromatography, Gel/methods , Dextrans , Hydroxyapatites , Male , Methods , Organic Chemicals , Rats , UltrafiltrationABSTRACT
Incorporation of 14C-glycine in plasmatic membrane proteins of the intact and regenerating liver was studied at the beginning of G1 period of the cell cycle. The electrophoretic study of 0.05 M Na2CO3 soluble plasmatic membrane proteins of the regenerating liver showed that maximal incorporation of 14C-glycine was associated with the proteins having molecular weight of about 60 000. The pattern of incorporation of 14C-glycine in different fractions of 0.05 M Na2CO3 insoluble proteins of the plasmatic membranes of the intact and regenrating liver did not differ essentially.
