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1.
Virology ; 237(1): 10-22, 1997 Oct 13.
Article in English | MEDLINE | ID: mdl-9344903

ABSTRACT

Previously, we identified an HBV binding factor (HBV-BF), a 50-kDa serum glycoprotein which interacts with HBV envelope proteins and which is also located in the membrane of normal human hepatocyte (A. Budkowska et al. (1993) J. Virol. 67, 4316). Here we show that HBV-BF is a neutral metalloproteinase which shares substrate specificity and properties with a newly described family of membrane type matrix metalloproteinases. HBV-BF treatment of the HBV resulted in the cleavage of the N-terminal part of the middle HBV envelope protein at the pre-S2(136-141) amino acid sequence VRGLYF/L (containing a single arginine cleavage site). HBV-BF affected the reactivity of the large HBV protein with pre-S1-specific MAbs, probably inducing the conformational change of the pre-S1 domain. The HBV-BF-digested virus remained morphologically intact with unchanged S antigenic determinants. The structural modifications of the viral envelope proteins induced by HBV-BF enabled cell membrane attachment and viral entry into the T-lymphocyte. Both processes were blocked by the metalloproteinase inhibitor 1,10 phenanthroline. Thus, the host-dependent proteolytic activation of the envelope proteins seems to be essential for the HBV entry into the cell. HBV-BF under a membrane bound or a secreted form could be (one of) the molecule(s) responsible for the HBV proteolytic activation.


Subject(s)
Hepatitis B virus/physiology , Metalloendopeptidases/physiology , T-Lymphocytes/virology , Viral Envelope Proteins/physiology , Virus Replication/physiology , Amino Acid Sequence , Cell Line , Humans , Molecular Sequence Data , Peptide Mapping , Viral Envelope Proteins/chemistry
2.
Arch Virol Suppl ; 4: 39-41, 1992.
Article in English | MEDLINE | ID: mdl-1450721

ABSTRACT

Using enzyme immune assay and immune electron microscopy, we have examined the sera of immune-suppressed anti-HBc negative HBV-infected patients for the presence of HBcAg. Our results suggest that free HBV core particles are absent or present only in minute amounts in the blood of chronic carriers and that at the most, only minimal amounts of core antigen are found on the surface of the virus particles.


Subject(s)
Hepatitis B Antibodies/blood , Hepatitis B Core Antigens/blood , Hepatitis B/immunology , Viremia/immunology , Carrier State , Humans , Immune Tolerance , Immunoenzyme Techniques , Microscopy, Immunoelectron
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