ABSTRACT
The localization of tetanospasmin and tetanolysin in C. tetani cells has been determined by the cytochemical method in the dynamics of the development of the microbial population. As shown in this investigation, tetanolysin, similarly to exotoxins, is released from microbial cells into the culture fluid mainly during the first 24 hours of cultivation, while tetanospasmin, similarly to endotoxins, is released only in the process of the destruction of these cells at the phase of the death of the microbial population.
Subject(s)
Antigens, Bacterial/immunology , Metalloendopeptidases , Tetanus Toxin/immunology , Animals , Antibodies, Bacterial/immunology , Antibodies, Bacterial/isolation & purification , Antigens, Surface/immunology , Chromatography, Affinity , Clostridium tetani/immunology , Clostridium tetani/ultrastructure , Cytoplasm/immunology , Cytoplasm/ultrastructure , Immunization , Immunochemistry , RabbitsSubject(s)
Metalloendopeptidases , Tetanus Toxin/isolation & purification , Animals , Bacterial Toxins/toxicity , Chromatography, Affinity , Chromatography, Gel , Clostridium tetani/physiology , Drug Evaluation, Preclinical , Immunization , Lethal Dose 50 , Mice , Neutralization Tests , Spectrophotometry, Ultraviolet , Tetanus/prevention & control , Tetanus Toxin/analysis , Tetanus Toxin/toxicity , Tetanus Toxoid/therapeutic useABSTRACT
The dynamics of the synthesis, transfer and excretion of toxin in C. novyi, growing in a liquid culture medium, have been studied on the level of bacterial ultrastructure by means of immunoferritin techniques modified by the authors. As revealed in this study, the basic mechanism of toxin excretion is realized by the active transfer of toxin through the enveloping structures after its accumulation in the periplasmatic space. In ageing cultures toxin may also be released in the process of bacteriolysis with the degradation of bacterial structures.
