ABSTRACT
The paper presents the results of computed morphometry of peripheral red blood cells in elderly patients with B12-deficiency anemia before treatment. The study was performed in the fine Romanovsky-stained peripheral blood smears, by using an ASPEK Russian hematological cell image analyzer. The objective quantitative characteristics of the parameters of the major megaloblastic hematopoiesis markers (macro- and megalocytes) in peripheral blood are given. The composition of peripheral blood was determined from the content of red blood cells with varying amounts of hemoglobin.
Subject(s)
Anemia/blood , Erythrocytes/pathology , Vitamin B 12 Deficiency/blood , Aged , Female , Humans , Image Processing, Computer-Assisted , Male , Middle AgedABSTRACT
This study describes an ELISA variant (Binding Inhibition ELISA, BI ELISA) for the quantitative determination of neutralization-relevant antibodies to polioviruses. The test differs from previously described analogs by utilizing polyclonal immune reagents: capture antibodies and biotin-labeled detecting antibodies. The BI ELISA was compared with the conventional neutralization test (NT) by testing live Sabin and wild-type poliovirus strains. The comparison of 68 serum samples taken from Oral Poliovirus Vaccine (OPV) recipients showed 100% specificity and sensitivity for Sabin 1 and Sabin 2, and 100% sensitivity and 97% specificity for Sabin 3. Good correlations (r = 0.7, 0.77, 0.65 for Sabin 1, 2, 3, respectively) were demonstrated between the NT and BI ELISA results. These results indicate that the BI ELISA can be used as a reliable alternative to the NT for determination of neutralization-relevant antibodies to polioviruses in vaccinees and in large-scale sero-epidemiological studies.
Subject(s)
Antibodies, Viral/biosynthesis , Enzyme-Linked Immunosorbent Assay/methods , Poliovirus Vaccine, Oral/immunology , Poliovirus/immunology , Poliovirus/metabolism , Animals , Antibodies, Viral/analysis , Antigens, Viral/analysis , Biotin/chemistry , Child , Cross Reactions , Female , Humans , Immune Sera/chemistry , Immune Sera/immunology , Immunoglobulin G/immunology , Indicators and Reagents , Male , Neutralization Tests , RabbitsABSTRACT
The authors have found that in chronic B-cell lymphoid leukemia and splenic lymphocytoma the proportion of irregular nuclei diminishes, in some patients split nuclei emerged. The proportion of irregular nuclei in lymphocytoma is significantly higher than that in chronic leukemia. The above diseases are characterized by disordered position of mitochondria in relation to cell center and of this center against nuclear invaginations. Microvilli on the cell surface become less numerous. It is suggested that in chronic lymphoproliferative diseases cytoskeleton in tumor cells may undergo structural and functional alterations.
Subject(s)
Lymphocytes/ultrastructure , Lymphoproliferative Disorders/pathology , Aged , Aged, 80 and over , Chronic Disease , Female , Humans , Male , Middle AgedABSTRACT
The authors share their experience gained with automation of hematologic investigations at a clinical diagnosis laboratory. A CDL information system, basing on personal computers, has been created, functioning in complex with hematologic autoanalyzers. The reports on the laboratory's activity are made automatically, as is the laboratory register and processing of applications for tests.
