Stimulation of antioxidant activity and γ-aminobutyric acid synthesis in germinated wheat grain Triticum aestivum L. by ultrasound: Increasing the nutritional value of the product.

The use of ultrasound to intensify the germination process of Triticum aestivum L. wheat was studied. This method of controlled germination can be used in several sectors of food industry, in particular in bakery. The effect of low-frequency ultrasound (20 kHz) at different intensities and duration on the germination process of Triticum aestivum L. wheat was systematically studied. We have found that 3-minute processing at 227 W/l output reduces the duration of wheat grain germination by 25% (12 ± 2 h) compared to the control samples. The use of ultrasound stimulated γ-aminobutyric acid (GABA) synthesis (18.9 ± 0.5 mg/100 g), increased the antioxidant activity (AOA) (2.86 ± 0.2 mg/g Trolox equivalents) and the amount of flavonoids (0.19 ± 0.03 mg QE/g). The SEM analysis of powder particles of whole-wheat flour made from wheat germinated with ultrasound exposure showed densely packed aggregates of protein matrix. To sum up, controlled ultrasound during wheat grain germination increases the amount of GABA and AOA. The whole-wheat flour is useful for food enrichment.

Development of Resource-Saving Biotechnology for the Production of a Feed Additive from Distiller's Wastes with Probiotic Properties.

The trend towards an increase in ethanol production on a global scale and the tightening of restrictive measures regarding the disposal of by-products from production increases the relevance of research in finding ways to process them. This study aimed to assess the effectiveness of a feed additive containing dried stillage fermented with pre-immobilized cultures of Lactobacillus pontis 67, Lb. casei 22, Lb. paracasei 104 when finishing steers. The dose of inoculum (3.0%) of a liquid probiotic drug based on the consortium Lb. pontis 67, Lb. casei 22, Lb. paracasei 104 was determined, cultivation time 24-30 hours at a bacterial titer of 10-10 CFU/ml, and the characteristics of fermented stillage and a feed additive based on it were established. Studies were conducted to assess the effectiveness of finishing when using fermented wheat stillage on the qualitative characteristics of the carcass of 13-month-old Kazakh white-headed steers on finishing. The studies were conducted in 2 groups: control and experimental (13% fermented stillage). The experimental group showed higher indicators of average daily gain (Р<0.05), carcass weight, and slaughter yield (P<0.05). According to the results of the conducted research, it can be concluded that the replacement of protein components of compound feed with stillage in an amount of 10% and a probiotic drug based on the bacteria Lb. pontis 67, Lb. casei 22, Lb. paracasei 104 in an amount of 3.0% contributes to an increase in weight gain when finishing steers by 9.1±0.3 kg, average daily gain by 417±2.0 g, and slaughter yield by 3.1±0.2% compared with the control group (P<0.05).

Ultrasound assisted acid hydrolyzed structure modification and loading of antioxidants on potato starch nanoparticles.

Starch is second most abundant biomaterial available after cellulose but the intensity of research on starch is less compared to cellulose. It is a carbohydrate based polymer synthesized in plants for the storage of the energy. Major percentage of starch is being utilized by food industries as raw material for giving texture, flavor, gelling, fat replacement etc. and also has multiple applications in different area due to its biological origin and properties. Native starch possesses low shear stress, poor thermal properties and less digestion resistance and retro-gradation. Thus, it has to be modified using physical, chemical, enzymatic and/or genetic treatments. Physical and chemical modifications using ultrasound and acid hydrolysis is time-efficient and effective process. These economical treatments are predominant for production of digestion resistant starch with increased shelf-life and thermal properties. Ultrasound assisted acid hydrolyzed starch (potato) exfoliates the native starch and modifies the structural arrangement. On acid treatment the amorphous nature of starch converted to crystalline nature. The physical and structural properties of the native starch were enhanced. The digestibility and structure of the modified starch effects on the double helices structure of starch. The size of the starch particle was changed from 1596 nm (Conventional) to 80 and 42 nm on ultrasonication and acid hydrolyzed ultrasonication approaches respectively. The crystallite of the particles was evaluated from XRD analysis. From TEM analysis the starch nanoparticles were found to have spherical morphology.

Ultrasound effects based on simulation of milk processing properties.

Efficient reconstitution followed by fermentation of nonfat (skimmed) dry milk is one of the most important aspects in manufacturing of various dairy products. As a first stage, dry milk is normally being reconstituted using conventional approaches such as heating and stirring the mixture. Nevertheless, we demonstrated, that low-frequency (22 ±â€¯1.65 kHz) ultrasound can be used as an alternative to the traditional methods of milk recovery. Low-frequency ultrasound was applied during pre-fermentation stage to improve the dispersity of the resulting milk product and to create good conditions for a post-ultrasound bacterial fermentation of milk. The applied ultrasound power was set in the range of 60-120 W/L. For the fermentation process we used: kefir fungi, fermenter of direct inoculation for yogurt LYOBAC YOYO and mixed culture for kefir LAT LC K. The effectiveness of ultrasound treatment was evaluated based on the accumulation of exopolysaccharides (EPS), vitamin C content, as well as antioxidant activity of the resulting milk product. The effect of ultrasound on microflora and the role of the specific types of bacteria such as Lactobacillus on the accumulation of biologically active compounds in the fermented milk products was studied with use of electron microscopy and MALDI - to FMS method. Overall, our results showed that reconstitution of dry milk with use of ultrasound improves further accumulation of biologically active compounds and rises the nutritional quality of the fermented product.

Ultrasound assisted synthesis of performic acid in a continuous flow microstructured reactor.

The present work establishes in depth study of ultrasound assisted preparation of performic acid (PFA) in a continuous flow microstructured reactor. The influence of various parameters viz. formic acid: hydrogen peroxide molar ratio, flow rate, temperature and catalyst loading on the PFA formation were studied in a continuous flow microstructured reactor. In a continuous microstructured reactor in the presence of ultrasonic irradiation, the formation of PFA was found to be dependent on the molar ratio of formic acid: hydrogen peroxide, flow rate of reactants, temperature and catalyst loading (Amberlite IR-120H). The optimized parameter values are 1:1M ratio, 50mL/h, 40°C and 471mg/cm3 respectively. Further, the performance of Amberlite IR-120H catalyst was evaluated for three successive cycles in continuous microstructured reactor. The performance of catalyst was found to be decreased with the usage of the catalyst and is attributed to neutralization of the sulfonic acid groups, catalyst shrinkage, or loss in pore sites. The experimental results revealed that, for an ultrasound assisted synthesis of PFA in continuous microstructured reactor the observed reaction time was even less than 10min. The observed intensification in the PFA synthesis process can be attributed to the intense collapse of the cavities formed at low temperature during ultrasonic irradiations, which further improved the heat and mass transfer rates with the formation of H2O2 during the reaction. The combined use of ultrasound and a continuous flow microstructured reactor has proved beneficial process of performic acid synthesis.