ABSTRACT
The chromosomal location of the porcine gene for glucose phosphate isomerase (GPI) was previously mapped to 6p 12----6q21 in the pig karyotype. The replication patterns and morphology of this chromosome are very similar to those of chromosome 14 in the rabbit karyotype. With combined in situ hybridization and RBG-band induction it was demonstrated that the porcine GPI-probe hybridized most frequently to 14p11----14q12 in the rabbit karyotype, indicating a close relationship between morphology, replication pattern and gene location.
Subject(s)
Rabbits/genetics , Swine/genetics , Animals , Chromosome Banding , Chromosome Mapping , Female , Glucose-6-Phosphate Isomerase/genetics , Karyotyping , Male , Nucleic Acid Hybridization , Species SpecificityABSTRACT
Nucleolar organizer regions (NORs) in the female dog karyotype are presented. Eight autosomes Nos. 5, 8, 14, 16, 19, 21, 32, and 37 showed active NORs. We observed a high incidence (50%) of metaphases with NOR association.
Subject(s)
Chromosome Banding , Dogs/genetics , Nucleolus Organizer Region/ultrastructure , Animals , Cells, Cultured , Female , Karyotyping , Metaphase , X ChromosomeABSTRACT
A method to preserve high resolution R-bands on human lymphocyte chromosomes after in situ hybridization to 3H-labelled probes and autoradiography is presented. The technique was used to re-examine the location of the known oncogene N-myc. Due to the high resolution power of the present technique, this gene could be located to subband 2p24.1 in the human karyotype.
Subject(s)
Chromosome Banding/methods , Chromosome Mapping , Chromosomes, Human, Pair 2 , Oncogenes , X Chromosome , Humans , Nucleic Acid HybridizationSubject(s)
Chromosome Aberrations , Isotope Labeling/methods , Lymphocytes , Organotechnetium Compounds , Oximes , Humans , Indium Radioisotopes/toxicity , Isotope Labeling/adverse effects , Kinetics , Leukemia, Lymphocytic, Chronic, B-Cell/pathology , Lymphocytes/radiation effects , Lymphocytes/ultrastructure , Lymphoma, Non-Hodgkin/pathology , Organometallic Compounds/toxicity , Organotechnetium Compounds/toxicity , Oximes/toxicity , Technetium Tc 99m Exametazime , Tropolone/analogs & derivatives , Tropolone/toxicityABSTRACT
Representative RBG-banded haploid karyotypes of the dog and diagrammatic representation of the banding patterns at the 420-band stage are presented.
Subject(s)
Dogs/genetics , Karyotyping/veterinary , Animals , Bisbenzimidazole , Bromodeoxyuridine/metabolism , Chromosome Banding , DNA Replication , Female , Fluorescent Dyes , HaploidyABSTRACT
A representative haploid R-banded karyotype of the domestic pig, and a diagrammatic representation of the banding patterns at the 550 band level are presented.
Subject(s)
Chromosome Banding , Swine/genetics , Animals , Cells, Cultured , Haploidy , KaryotypingABSTRACT
Representative haploid R-banded karyotypes of the laboratory rat and diagrammatic representation of the banding patterns at the 525-band level are presented.