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1.
Phys Rev Lett ; 92(16): 163201, 2004 Apr 23.
Article in English | MEDLINE | ID: mdl-15169227

ABSTRACT

We present a detailed theoretical investigation of the coherence properties of beam splitters and Mach-Zehnder interferometers for guided neutral atoms. We show that such a setup permits coherent wave packet splitting and leads to the appearance of interference fringes for both single-mode and thermal input states, evidencing thus the robustness of the interferometer.

2.
Phys Rev Lett ; 87(12): 123601, 2001 Sep 17.
Article in English | MEDLINE | ID: mdl-11580510

ABSTRACT

We propose to transfer quantum correlations from atoms to light by Raman scattering of a strong laser pulse on a spin-squeezed atomic sample. We prove that the emission is restricted to a single field mode which perfectly inherits the quantum correlations of the atomic system.

3.
Protein Expr Purif ; 22(2): 189-99, 2001 Jul.
Article in English | MEDLINE | ID: mdl-11437594

ABSTRACT

Hexose oxidase (D-hexose:O(2)-oxidoreductase, EC 1.1.3.5, HOX) normally found in the red alga Chondrus crispus was produced heterologously in different host systems. Full-length HOX polypeptide was produced in Escherichia coli, but no HOX activity could be detected. In contrast, active HOX could be produced in the methylotrophic yeast Pichia pastoris. Several growth physiological and genetic approaches for optimization of hexose oxidase production in P. pastoris were investigated. Our results indicate that specific growth conditions are essential in order to produce active HOX with the correct conformation. Furthermore, HOX seems to be activated by proteolytic cleavage of the full-length polypeptide chain into two fragments, which remain physically associated. Attempts to direct HOX to the extracellular compartment using the widely used secretion signals from Saccharomyces cerevisiae invertase or alpha-mating factor failed. However, we show in this study that HOX is transported out of P. pastoris via a hitherto unknown mechanism and that it is possible to enhance this secretion by mutagenesis from below the detection limit to at least 250 mg extracellular enzyme per liter.


Subject(s)
Alcohol Oxidoreductases/genetics , Pichia/enzymology , Pichia/genetics , Recombinant Proteins/biosynthesis , Rhodophyta/enzymology , Rhodophyta/genetics , Alcohol Oxidoreductases/immunology , Alcohol Oxidoreductases/metabolism , Alcohol Oxidoreductases/radiation effects , Antibody Formation/genetics , Antibody Specificity/genetics , Blotting, Western , Cloning, Molecular , Copper/metabolism , Culture Media, Conditioned/metabolism , Endopeptidases/metabolism , Enzyme Activation/genetics , Escherichia coli/enzymology , Escherichia coli/genetics , Flavin-Adenine Dinucleotide/metabolism , Gene Expression Regulation, Enzymologic , Genetic Vectors/chemical synthesis , Glycoside Hydrolases/genetics , Glycoside Hydrolases/metabolism , Hydrolysis , Mating Factor , Mutagenesis , Peptides/genetics , Peptides/metabolism , Pichia/radiation effects , Protein Folding , Protein Sorting Signals/genetics , Recombinant Proteins/immunology , Recombinant Proteins/metabolism , Recombinant Proteins/radiation effects , Rhodophyta/physiology , Ultraviolet Rays , beta-Fructofuranosidase
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