Subject(s)
Limulus Test/standards , Animals , Canada , Endotoxins/analysis , Government Agencies , Humans , RabbitsABSTRACT
A high-speed liquid chromatographic system is described, which can be used for the simultaneous identification of the anabolic steroid methandrostenolone and its impurities and the quantitation of each of these compounds. Separation is effected by adsorption chromatography on a slurry-packed microparticulate silica gel column.
Subject(s)
Methandrostenolone/analysis , Steroids/analysis , Chromatography , Methods , Tablets/analysisABSTRACT
A sensitive, specific, high-speed liquid chromatographic procedure is described for the simultaneous determination of phenylbutazone and its metabolite, oxyphenbutazone, in plasma. Acidified plasma is partitioned with cyclohexane-ether (1:1) containing the 2,4-dinitrophenylhydrazone of 3,4-dimethoxybenzaldehyde as an internal standard. The organic extract is reduced to dryness, the resulting residue is redissolved in chloroform, and aliquots of this solution are chromatographed on an adsorption column, using a mobile phase of 0.002% acetic acid and 23.0% tetrahydrofuran in n-hexane at 35 degrees. Use of a UV detector permits quantitative analysis of samples containing less than 0.25 mug/ml of phenylbutazone or oxyphenbutazone.
Subject(s)
Oxyphenbutazone/blood , Phenylbutazone/blood , Chromatography , Chromatography, Gas , Humans , Male , Methods , Spectrophotometry, Ultraviolet , Time FactorsABSTRACT
A rapid, precise high-speed liquid chromatographic procedure for the simultaneous determination of tetracycline and rolitetracycline in rolitetracycline formulations is described. Samples are dissolved in water, chilled to 0 degrees, and chromatographed on a pellicular cation-exchange resin, The specificity of this method represents a significant improvement over present analytical procedures, which fail to differentiate between rolitetracycline and its hydrolysis product, tetracycline, in these formulations.
Subject(s)
Rolitetracycline/analysis , Tetracycline/analysis , Biological Assay , Chromatography, Ion Exchange/methods , Nitrates/analysis , Spectrophotometry, Ultraviolet , Tetracyclines , Time FactorsSubject(s)
Phenylbutazone/blood , Autoanalysis , Chromatography , Humans , Hydrogen-Ion Concentration , Male , Methods , Solvents , Spectrophotometry, Ultraviolet , Time FactorsSubject(s)
Autoanalysis , Chromatography , Estrogens/analysis , 17-Ketosteroids/analysis , Animals , Chemical Phenomena , Chemistry , Chromatography, Gas , Chromatography, Paper , Chromatography, Thin Layer , Esters/analysis , Estranes/analysis , Estrogens, Conjugated (USP)/analysis , Estrone/analysis , Glycols , Horses , Hydroxysteroids/analysis , Indicators and Reagents , Methods , Nitriles , Silicones , SolventsABSTRACT
The high-speed liquid chromatographic behavior of 15 tetracyclines on anion- and cation-exchange columns is described and illustrated by typical separations of a number of tetracycline derivatives on several systems, as well as by the resolution of tetracycline from its degradation products epi-, epianhydro-, and anhydrotetracycline. These serve as a basis for a rapid, convenient, and precise method for the direct detection of the tetracycline antibiotics and their degradation products.
