Characterization of the bacterial flora isolated from a pilot-scale lagoon processing swine manure.

The bacterial flora of an experimental plant that processes liquid swine manure by an aerated compartmented (multistage) lagoon system has been studied. The total flora is characterized by a larger number of oligotrophic bacteria than eutrophic ones. Each compartment displays a specific flora, different from the flora in the manure, and consisting of a complex assembly of Gram-negative and Gram-positive ubiquitous species, such as Aeromonas spp. and Pseudomonas spp., and specialized species, such as Sphingobacterium spp. and Corynebacterium spp. The fecal indicator microorganisms have been shown to disappear in the course of the processing. A significant population of nitrifying bacteria has been observed at levels up to 10(4) bacteria.mL-1.

[Surgical indications for primary vesico-ureteral reflux in adult candidates for renal transplantation]. / L'indication opératoire concernant le reflux vésico-rénal primitif de l'adulte chez un candidat à la transplantation rénale.

Eight out of thirty five candidates for renal transplantation at the Bordeaux transplantation center, between 1975 and 1983, all presenting with primary vesico-ureteric reflux, underwent nephro-ureterectomy. The remaining twenty seven were not treated surgically. The authors consider that no operation is required when the ureter is narrow and uninfected, but that dilated refluant and infected ureters should be treated by nephro-ureterectomy, at least unilaterally. In that event, the operation is performed in two stages, with the second stage being performed at the same time as the transplantation.

[Primary vesico-ureteral reflux in adults. 1977-1982. Department experience of 6-years]. / Le reflux vésico-rénal primitif de l'adulte. 1977-1982. Expérience du service sur six ans.

The study of a series of 182 ureters (in 102 patients) treated in the Tripode Hospital, Bordeaux, highlights the following facts: as a diagnostic tool, cystography, if performed only once, is not reliable; on the other hand, isotopic cystography and endoscopic data are important adjuncts. As regards the management of these cases, 109 vesico-ureteric reimplantations were performed, 83 using Cohen's technique and 26 using Leadbetter's associated in some cases with a unicornous bladder. The results were excellent except in patients presenting with a severe renal insufficiency. Radical surgery was required in twenty cases. Fifty three patients were not treated surgically, but, when renal function is satisfactory, this attitude seems to offer more drawbacks than benefits.

Development of a culture medium for large-scale production of cellulolytic enzymes by Trichoderma reesei.

Culture filtrates of CL-847 strain of Trichoderma reesei grown on different carbon sources have been compared. The highest enzyme production is obtained with Whatman C 41 cellulose: 17.9 mg/mL of soluble proteins and 13.7 units of filter paper (FP) activity. Wood pulps gave lower production values and more viscous culture media. About one-third of maximal enzyme production is obtained on lactose as the sole carbon source. Addition of 0.5% cellulosic inducer to 6% lactose media enhances enzyme production up to the following levels: 14.1 mg/mL of soluble proteins and 8.4 units of FP activity.