Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 20 de 20
Filter
Add more filters










Publication year range
1.
Biochim Biophys Acta Biomembr ; 1863(1): 183488, 2021 01 01.
Article in English | MEDLINE | ID: mdl-33065135

ABSTRACT

Gram-negative bacteria export a large variety of antimicrobial compounds by forming two-membrane spanning tripartite multidrug efflux systems composed of an inner membrane transporter, an outer membrane channel and a periplasmic adaptor protein. Here we present the co-expression, purification and first electron microscopy insights of the Escherichia coli EmrAB-TolC tripartite Major Facilitator Superfamily (MSF) efflux system as a whole complex stabilized by Amphipol polymer. The structure reveals a 33 nm long complex delineated by the Amphipol belt at both extremities. Comparison of projection structures of EmrAB-TolC and AcrAB-TolC indicates that the outer membrane protein TolC linked to the periplasmic adaptor EmrA protein form an extended periplasmic canal. The overall length of EmrAB-TolC complex is similar to that of AcrAB-TolC with a probable tip-to-tip interaction between EmrA and TolC unveiling how the adaptor protein connects TolC and EmrB embedded in the inner membrane.


Subject(s)
Bacterial Outer Membrane Proteins/chemistry , Escherichia coli Proteins/chemistry , Escherichia coli/chemistry , Membrane Proteins/chemistry , Membrane Transport Proteins/chemistry , Multiprotein Complexes/chemistry , Bacterial Outer Membrane Proteins/genetics , Bacterial Outer Membrane Proteins/metabolism , Escherichia coli/genetics , Escherichia coli/metabolism , Escherichia coli Proteins/genetics , Escherichia coli Proteins/metabolism , Membrane Proteins/genetics , Membrane Proteins/metabolism , Membrane Transport Proteins/genetics , Membrane Transport Proteins/metabolism , Multiprotein Complexes/genetics , Multiprotein Complexes/metabolism , Protein Structure, Quaternary
2.
Biochim Biophys Acta Gen Subj ; 1863(5): 830-838, 2019 05.
Article in English | MEDLINE | ID: mdl-30768959

ABSTRACT

Atherosclerosis is a lipid disease characterized by accumulation of low density lipoprotein (LDL) in the artery wall. The transport of LDL across the endothelium of coronary artery is an initiating event of atherosclerosis, whose mechanism remains poorly understood. In the last decade, it has been shown that in caveolin-1 (Cav-1) deficient mice, LDL infiltration in aorta wall is decreased and CD36 expression in aortas is down-regulated, leading to regression of atherosclerotic lesions. In the present study, we show that native LDL endocytosis is decreased in endothelial cells deficient in Cav-1 or CD36. We demonstrate that Cav-1 and CD36 interact in caveolae-rich domains by different biochemical approaches. In addition, confocal microscopy reveals some colocalization of Cav-1 with CD36. These findings indicate that caveolae and CD36 are involved in native LDL endocytosis and suggest that CD36 might be a good candidate for the transport of native LDL across the endothelium, an early event in atherosclerosis.


Subject(s)
CD36 Antigens/metabolism , Caveolin 1/metabolism , Endothelial Cells/metabolism , Lipoproteins, LDL/metabolism , CD36 Antigens/chemistry , Caveolin 1/chemistry , Cell Proliferation , Cells, Cultured , Endocytosis , Humans , Lipoproteins, LDL/chemistry , Microscopy, Fluorescence , Optical Imaging
3.
Exp Cell Res ; 370(2): 623-631, 2018 09 15.
Article in English | MEDLINE | ID: mdl-30031131

ABSTRACT

Chronic diseases are characterized by the production of pro-inflammatory cytokines such than TNF-α and are frequently correlated with muscle wasting conditions. Among the pleiotropic effects of TNF-α within the cell, its binding to TNFR1 receptor has been shown to activate sphingomyelinases leading to the production of ceramides. Sphingomyelinases and TNF receptor have been localized within caveolae which are specialized RAFT enriched in cholesterol and sphingolipids. Because of their inverted omega shape, maintained by the oligomerization of specialized proteins, caveolins and cavins, caveolae serve as membrane reservoir therefore providing mechanical protection to plasma membranes. Although sphingolipids metabolites, caveolins and TNF-α/TNFR1 have been shown to independently interfere with muscle physiology, no data have clearly demonstrated their concerted action on muscle cell regeneration. In this context, our study aimed at studying the molecular mechanisms induced by TNF-α at the level of caveolae in LHCN-M2 human muscle satellite cells. Here we showed that TNF-α-induced production of ROS and nSMase activation requires caveolin. More strikingly, we have demonstrated that TNF-α induces the formation of additional caveolae at the plasma membrane of myoblasts. Furthermore, TNF-α prevents myoblast fusion suggesting that inflammation could modulate caveolae organization/function and satellite cell function.


Subject(s)
Caveolae/metabolism , Muscle Fibers, Skeletal/metabolism , Tumor Necrosis Factor-alpha/metabolism , Tumor Necrosis Factor-alpha/pharmacology , Caveolin 1/metabolism , Cell Membrane/drug effects , Cell Membrane/metabolism , Cholesterol/metabolism , Humans , Myoblasts/drug effects , Myoblasts/metabolism , RNA, Small Interfering/metabolism , Receptors, Tumor Necrosis Factor, Type I , Signal Transduction/drug effects
4.
Nanomedicine ; 14(1): 141-151, 2018 01.
Article in English | MEDLINE | ID: mdl-28939489

ABSTRACT

Delivery of biologically active proteins into cells is emerging as important strategy for many applications. Previous experiments have shown that lipoaminoglycosides were capable of delivery of the anti-cytokeratin8 antibody (anti-K8) but only when formulated with lipid helpers potentially leading to toxicity from excess lipids. Here, we optimized anti-K8 delivery with various lipoaminoglycosides in the absence of a lipid helper. Results led to the identification of the aminoglycoside lipid dioleyl phosphoramido ribostamycin (DOPRI) as a potent intracellular delivery system for anti-K8. Electron microscopy revealed that delivered anti-K8 molecules were bound to intermediate filaments in cells. Anti-K8 was bound to the surface of DOPRI vesicles without perturbing lipid organization. Macropinocytosis and caveolin mediated endocytosis contributed to anti-K8 internalization and to filament labeling with a major contribution being made by the caveolin pathway. The results showed that the unique properties of DOPRI were sufficient for efficient intracellular protein delivery without requiring lipid helpers.


Subject(s)
Anti-Bacterial Agents/pharmacology , Antibodies/metabolism , Drug Delivery Systems , Endocytosis , Ribostamycin/pharmacology , Anti-Bacterial Agents/chemistry , Antibodies/administration & dosage , Antibodies/immunology , HeLa Cells , Humans , Keratin-8/immunology , Ribostamycin/chemistry
5.
PLoS One ; 10(3): e0122654, 2015.
Article in English | MEDLINE | ID: mdl-25799323

ABSTRACT

Increased level of oxidative stress, a major actor of cellular aging, impairs the regenerative capacity of skeletal muscle and leads to the reduction in the number and size of muscle fibers causing sarcopenia. Caveolin 1 is the major component of caveolae, small membrane invaginations involved in signaling and endocytic trafficking. Their role has recently expanded to mechanosensing and to the regulation of oxidative stress-induced pathways. Here, we increased the amount of reactive oxidative species in myoblasts by addition of hydrogen peroxide (H2O2) at non-toxic concentrations. The expression level of caveolin 1 was significantly decreased as early as 10 min after 500 µM H2O2 treatment. This reduction was not observed in the presence of a proteasome inhibitor, suggesting that caveolin 1 was rapidly degraded by the proteasome. In spite of caveolin 1 decrease, caveolae were still able to assemble at the plasma membrane. Their functions however were significantly perturbed by oxidative stress. Endocytosis of a ceramide analog monitored by flow cytometry was significantly diminished after H2O2 treatment, indicating that oxidative stress impaired its selective internalization via caveolae. The contribution of caveolae to the plasma membrane reservoir has been monitored after osmotic cell swelling. H2O2 treatment increased membrane fragility revealing that treated cells were more sensitive to an acute mechanical stress. Altogether, our results indicate that H2O2 decreased caveolin 1 expression and impaired caveolae functions. These data give new insights on age-related deficiencies in skeletal muscle.


Subject(s)
Caveolae/metabolism , Caveolin 1/metabolism , Hydrogen Peroxide/toxicity , Oxidative Stress/drug effects , Animals , Caveolin 1/antagonists & inhibitors , Caveolin 1/genetics , Cell Line , Cell Membrane/metabolism , Cell Survival/drug effects , Down-Regulation/drug effects , Endocytosis/drug effects , Flow Cytometry , Mice , Microscopy, Electron , Myoblasts/cytology , Myoblasts/metabolism , Protease Inhibitors/pharmacology , Proteasome Endopeptidase Complex/chemistry , Proteasome Endopeptidase Complex/metabolism , RNA Interference , RNA, Small Interfering/metabolism , Reactive Oxygen Species/metabolism
6.
Int J Nanomedicine ; 10: 1479-92, 2015.
Article in English | MEDLINE | ID: mdl-25733836

ABSTRACT

The use of silica nanoparticles for their cellular uptake capability opens up new fields in biomedical research. Among the toxicological effects associated with their internalization, silica nanoparticles induce apoptosis that has been recently reported as a biochemical cue required for muscle regeneration. To assess whether silica nanoparticles could affect muscle regeneration, we used the C2C12 muscle cell line to study the uptake of fluorescently labeled NPs and their cellular trafficking over a long period. Using inhibitors of endocytosis, we determined that the NP uptake was an energy-dependent process mainly involving macropinocytosis and clathrin-mediated pathway. NPs were eventually clustered in lysosomal structures. Myoblasts containing NPs were capable of differentiation into myotubes, and after 7 days, electron microscopy revealed that the NPs remained primarily within lysosomes. The presence of NPs stimulated the formation of myotubes in a dose-dependent manner. NP internalization induced an increase of apoptotic myoblasts required for myoblast fusion. At noncytotoxic doses, the NP uptake by skeletal muscle cells did not prevent their differentiation into myotubes but, instead, enhanced the cell fusion.


Subject(s)
Muscle Fibers, Skeletal , Myoblasts , Nanoparticles/chemistry , Silicon Dioxide , Cell Differentiation/drug effects , Cell Line , Humans , Muscle Fibers, Skeletal/drug effects , Muscle Fibers, Skeletal/metabolism , Myoblasts/drug effects , Silicon Dioxide/chemistry , Silicon Dioxide/metabolism , Silicon Dioxide/pharmacology
7.
Phytother Res ; 27(10): 1529-35, 2013 Oct.
Article in English | MEDLINE | ID: mdl-23192879

ABSTRACT

The gradual loss of muscle mass affecting all the elderly (sarcopenia) is most likely due to a decreased number and/or function of satellite cells. Accumulation of reactive oxygen species (ROS) has been clearly correlated to sarcopenia and could contribute to the impairment of satellite cell function. In this study, we analyzed the protective mechanism of action of a natural pine bark extract (Oligopin®) in human muscle satellite cells exposed to oxidative stress (H2O2). This polyphenol belongs to the flavonoid family and was able to abolish the H2 O2-induced apoptotic cell death. A large-scale proteomic strategy allowed us to identify several proteins that may function as early regulators of ROS-mediated events in muscle cells. Interestingly, we identified the stress chaperone heat shock protein beta-1, a main protector of muscle necrosis, as a target of Oligopin® and showed that this polyphenol was able to modulate its stress induced phosphorylation.


Subject(s)
Antioxidants/pharmacology , HSP27 Heat-Shock Proteins/metabolism , Pinus/chemistry , Plant Extracts/pharmacology , Satellite Cells, Skeletal Muscle/drug effects , Apoptosis/drug effects , Cell Line , Heat-Shock Proteins , Humans , Hydrogen Peroxide/pharmacology , Molecular Chaperones , Oxidative Stress/drug effects , Phosphorylation , Plant Bark/chemistry , Polyphenols/pharmacology , Proteomics , Sarcopenia , Satellite Cells, Skeletal Muscle/metabolism
8.
J Biol Chem ; 285(17): 12670-83, 2010 Apr 23.
Article in English | MEDLINE | ID: mdl-20139084

ABSTRACT

Calpain 3 is a calcium-dependent cysteine protease that is primarily expressed in skeletal muscle and is implicated in limb girdle muscular dystrophy type 2A. To date, its best characterized function is located within the sarcomere, but this protease is found in other cellular compartments, which suggests that it exerts multiple roles. Here, we present evidence that calpain 3 is involved in the myogenic differentiation process. In the course of in vitro culture of myoblasts to fully differentiated myotubes, a population of quiescent undifferentiated "reserve cells" are maintained. These reserve cells are closely related to satellite cells responsible for adult muscle regeneration. In the present work, we observe that reserve cells express higher levels of endogenous Capn3 mRNA than proliferating myoblasts. We show that calpain 3 participates in the establishment of the pool of reserve cells by decreasing the transcriptional activity of the key myogenic regulator MyoD via proteolysis independently of the ubiquitin-proteasome degradation pathway. Our results identify calpain 3 as a potential new player in the muscular regeneration process by promoting renewal of the satellite cell compartment.


Subject(s)
Calpain/metabolism , Cell Differentiation , Down-Regulation , Muscle Development , Muscle Fibers, Skeletal/metabolism , Muscle Proteins/metabolism , MyoD Protein/metabolism , Myoblasts/metabolism , Calpain/genetics , Cell Line , Humans , Muscle Proteins/genetics , Muscular Dystrophies/genetics , Muscular Dystrophies/metabolism , MyoD Protein/genetics , Proteasome Endopeptidase Complex/genetics , Proteasome Endopeptidase Complex/metabolism , Regeneration/genetics , Satellite Cells, Skeletal Muscle/metabolism , Transcription, Genetic/genetics , Ubiquitin/metabolism
9.
Exp Cell Res ; 316(1): 115-25, 2010 Jan 01.
Article in English | MEDLINE | ID: mdl-19651121

ABSTRACT

The reduced regenerative potential of muscle fibres, most likely due to a decreased number and/or function of satellite cells, could play a significant role in the progression of muscle ageing. Accumulation of reactive oxygen species has been clearly correlated to sarcopenia and could contribute to the impairment of satellite cell function. In this work we have investigated the effect of oxidative stress generated by hydrogen peroxide in cultured human skeletal muscle satellite cells. We specifically focused on the activity and regulation of calpains. These calcium-dependent proteases are known to regulate many transduction pathways including apoptosis and play a critical role in satellite cell function. In our experimental conditions, which induce an increase in calcium concentration, protein oxidation and apoptotic cell death, a significant up-regulation of calpain expression and activity were observed and ATP synthase, a major component of the respiratory chain, was identified as a calpain target. Interestingly we were able to protect the cells from these H(2)O(2)-induced effects and prevent calpain up-regulation with a natural antioxidant extracted from pine bark (Oligopin). These data strongly suggest that oxidative stress could impair satellite cell functionality via calpain-dependent pathways and that an antioxidant such as Oligopin could prevent apoptosis and calpain activation.


Subject(s)
Calcium Signaling/physiology , Calpain/metabolism , Myoblasts/metabolism , Oxidative Stress/physiology , Up-Regulation/physiology , Antioxidants/pharmacology , Apoptosis/drug effects , Calcium Signaling/drug effects , Calpain/antagonists & inhibitors , Calpain/genetics , Cell Survival/drug effects , Cells, Cultured , Cysteine Proteinase Inhibitors/pharmacology , Cytoplasm/metabolism , Cytoplasmic Structures/metabolism , Dipeptides/pharmacology , Flavonoids/pharmacology , Gene Expression/drug effects , Gene Expression/genetics , Humans , Hydrogen Peroxide/pharmacology , Mitochondrial Proton-Translocating ATPases/metabolism , Myoblasts/drug effects , Myoblasts/enzymology , Oxidative Stress/drug effects , Phenols/pharmacology , Pinus/chemistry , Plant Extracts/pharmacology , Polyphenols , Protein Carbonylation/drug effects , Satellite Cells, Skeletal Muscle/drug effects , Satellite Cells, Skeletal Muscle/metabolism , p38 Mitogen-Activated Protein Kinases/metabolism
10.
Exp Gerontol ; 42(11): 1088-98, 2007 Nov.
Article in English | MEDLINE | ID: mdl-17937979

ABSTRACT

Aging is associated with a progressive and involuntary loss of muscle mass also known as sarcopenia. This condition represents a major public health concern with high socio-economics implications. Although sarcopenia is well documented, the aetiology of this condition still remains poorly understood. Calpains are ubiquitous proteases regulated in part by a specific inhibitor, calpastatin. They are well known to have major implications in muscle growth and differentiation. The aim of the present study was to determine if this proteolytic system could be involved in the phenotype associated with sarcopenia. Calpains and calpastatin levels, subcellular distributions and activities were compared between muscles from 3 and 24 months old rats. Altogether, the results we obtained showed an overall increase in calpain activities associated with muscle aging. These findings suggest that the calcium-dependent proteolytic system is indeed involved in sarcopenia.


Subject(s)
Aging/metabolism , Calcium/metabolism , Muscle, Skeletal/metabolism , Protein Processing, Post-Translational , Animals , Biomarkers/analysis , Calcium-Binding Proteins/analysis , Calcium-Binding Proteins/metabolism , Calpain/analysis , Calpain/metabolism , Male , Muscle, Skeletal/chemistry , Rats , Rats, Sprague-Dawley
11.
Proteomics ; 7(18): 3289-98, 2007 Sep.
Article in English | MEDLINE | ID: mdl-17849407

ABSTRACT

Caveolae are specialised RAFTs (detergent-resistant membrane microdomains enriched in cholesterol and glycosphingolipids). Caveolin, the main caveolae protein, is essential to the organisation of proteins and lipids, and interacts with numerous mediating proteins through a 'Caveolin Scalfolding Domain'. Consequently, caveolae play a major role in signal transduction and appear to be veritable signalling platforms. In muscle cells, caveolae are essential for fusion and differentiation, and are also implicated in a type of muscular dystrophy (LGMD1C). In a preceding work, we demonstrated the presence of active milli-calpain (m-calpain) in myotube caveolae. Calpains are calcium-dependent proteases involved in several cellular processes, including myoblast fusion and migration, PKC-mediated intracellular signalling and remodelling of the cytoskeleton. For the first time, we have proved the cholesterol-dependent localisation of m-calpain in the caveolae of C(2)C(12) myotubes. Calpain-dependent caveolae involvement in myoblast fusion was also strongly suggested. Furthermore, eight differentially expressed caveolae associated proteins were identified by 2-DE and LC-MS/MS analyses using an m-calpain antisense strategy. This proteomic study also demonstrates the action of m-calpain on vimentin, desmin and vinculin in myotube caveolae and suggests m-calpain's role in several mitochondrial pathways.


Subject(s)
Calpain/metabolism , Caveolae/metabolism , Muscle Fibers, Skeletal/metabolism , Proteome , Amino Acid Sequence , Base Sequence , Blotting, Western , Chromatography, Liquid , Electrophoresis, Gel, Two-Dimensional , Immunohistochemistry , Molecular Sequence Data , Muscle Fibers, Skeletal/drug effects , Protein Kinase C/metabolism , RNA, Antisense/pharmacology , Signal Transduction , Tandem Mass Spectrometry
12.
Int J Biochem Cell Biol ; 37(9): 1900-10, 2005 Sep.
Article in English | MEDLINE | ID: mdl-15923133

ABSTRACT

We have previously shown that calpain promotes myoblast fusion by acting on protein kinase C-alpha and the cytosolic phosphorylated form of MARCKS. In other cell types, various isoforms of calpain, PKC alpha and MARCKS were found associated with caveolae. These vesicular invaginations of the plasma membrane are essential for myoblast fusion and differentiation. We have isolated caveolae from myoblasts and studied the presence of calpain isoforms and their possible effects on signalling mediated by caveolae-associated PKC. Our results show that milli-calpain co-localizes with myoblast caveolae. Futhermore we provide evidence, using a calcium ionophore and a specific inhibitor of calpains (calpastatin peptide), that milli-calpain reduces the PKC alpha and MARCKS content in these structures. Purified milli-calpain causes the appearance of the active catalytic fragment of PKC alpha (PKM), without having an effect on MARCKS. Addition of phorbol myristate acetate, an activator of PKC, induces tranlocation of PKC alpha towards caveolae and results in a significant reduction of MARCKS associated with caveolae. This phenomenon is not observed when a PKC alpha inhibitor is added at the same time. We conclude that the presence of biologically active milli-calpain within myoblast caveolae induces, in a PKC alpha-dependent manner, MARCKS translocation towards the cytosol. Such a localised signalling event may be essential for myoblast fusion and differentiation.


Subject(s)
Calpain/metabolism , Caveolae/metabolism , Cytosol/metabolism , Intracellular Signaling Peptides and Proteins/metabolism , Membrane Proteins/metabolism , Myoblasts/metabolism , Protein Kinase C/metabolism , Signal Transduction , Animals , Carcinogens/pharmacology , Cell Compartmentation , Cell Fusion , Mice , Myoblasts/cytology , Myristoylated Alanine-Rich C Kinase Substrate , Protein Kinase C-alpha , Tetradecanoylphorbol Acetate/pharmacology
13.
Biochem J ; 382(Pt 3): 1015-23, 2004 Sep 15.
Article in English | MEDLINE | ID: mdl-15239673

ABSTRACT

MARCKS (myristoylated alanine-rich C kinase substrate) is a major cytoskeletal protein substrate of PKC (protein kinase C) whose cellular functions are still unclear. However numerous studies have implicated MARCKS in the stabilization of cytoskeletal structures during cell differentiation. The present study was performed to investigate the potential role of Ca(2+)-dependent proteinases (calpains) during myogenesis via proteolysis of MARCKS. It was first demonstrated that MARCKS is a calpain substrate in vitro. Then, the subcellular expression of MARCKS was examined during the myogenesis process. Under such conditions, there was a significant decrease in MARCKS expression associated with the appearance of a 55 kDa proteolytic fragment at the time of intense fusion. The addition of calpastatin peptide, a specific calpain inhibitor, induced a significant decrease in the appearance of this fragment. Interestingly, MARCKS proteolysis was dependent of its phosphorylation by the conventional PKCalpha. Finally, ectopic expression of MARCKS significantly decreased the myoblast fusion process, while reduced expression of the protein with antisense oligonucleotides increased the fusion. Altogether, these data demonstrate that MARCKS proteolysis is necessary for the fusion of myoblasts and that cleavage of the protein by calpains is involved in this regulation.


Subject(s)
Calpain/metabolism , Intracellular Signaling Peptides and Proteins/physiology , Membrane Proteins/physiology , Myoblasts/physiology , Protein Kinase C/metabolism , Animals , Cell Fusion , Cell Line , Cytoskeleton/physiology , Intracellular Signaling Peptides and Proteins/metabolism , Membrane Proteins/metabolism , Mice , Muscle Development/physiology , Myristoylated Alanine-Rich C Kinase Substrate , Protein Kinase C-alpha , Recombinant Proteins/metabolism , Subcellular Fractions/metabolism
14.
Int J Biochem Cell Biol ; 36(4): 728-43, 2004 Apr.
Article in English | MEDLINE | ID: mdl-15010335

ABSTRACT

Several studies have already demonstrated that micro- and milli-calpains (CAPN 1-CAPN 2), calcium-dependent intracellular cysteine-proteases are involved in many biological phenomenon including muscle growth and development. More particularly, recent studies have demonstrated that milli-calpain is implicated in myoblast fusion. Moreover, in primary muscle cells, these proteases do not appear simultaneously throughout muscle cell differentiation. Because micro- and milli-calpains do not have the same intracellular localization, it appears likely that these two calcium-dependent proteases have different biological roles during muscle cell differentiation. The goal of this study is to determine the role of micro-calpain. We therefore, have developed a muscle cell line in which micro-calpain is over-expressed, using the inducible Tet Regulated Expression System. The outcome is observed by following the behavior of different proteins, considered to be potential substrates of the protease. The present study shows important decreases in the expression level of ezrin (68%), vimentin (64%) and caveolin 3 (76%) whereas many other cytoskeletal proteins remain remarkably stable. Concerning the myogenic transcription factors, only the level of myogenin decreased (59%) after the over-expression of micro-calpain. Ultra structural studies have shown that the myofibrils formed near the cell periphery are normally oriented, lying along the longitudinal axis. This regularity is lost progressively towards the cell center where the cytoskeleton presented an increasing disorganization. All these results indicate that micro-calpain is involved in regulation pathway of myogenesis via at least its action on ezrin, vimentin, caveolin 3 and myogenin, a muscle transcription factor.


Subject(s)
Calpain/physiology , Muscle Cells/metabolism , Animals , Calpain/genetics , Caveolin 3 , Caveolins/genetics , Caveolins/metabolism , Cell Culture Techniques , Cell Differentiation , Cloning, Molecular , Cytoskeletal Proteins , DNA, Complementary/genetics , DNA-Binding Proteins/genetics , DNA-Binding Proteins/metabolism , Desmin/genetics , Desmin/metabolism , Doxycycline/pharmacology , Fibronectins/genetics , Fibronectins/metabolism , Gene Expression Regulation , Humans , Mice , Muscle Cells/physiology , Muscle Cells/ultrastructure , Muscle Fibers, Skeletal/metabolism , Muscle Fibers, Skeletal/ultrastructure , Muscle Proteins/genetics , Muscle Proteins/metabolism , MyoD Protein/genetics , MyoD Protein/metabolism , Myogenic Regulatory Factor 5 , Phosphoproteins/genetics , Phosphoproteins/metabolism , Trans-Activators/genetics , Trans-Activators/metabolism , Vimentin/genetics , Vimentin/metabolism
15.
Exp Cell Res ; 292(1): 187-200, 2004 Jan 01.
Article in English | MEDLINE | ID: mdl-14720518

ABSTRACT

Cell migration is a fundamental cellular function particularly during skeletal muscle development. Ubiquitous calpains are well known to play a pivotal role during muscle differentiation, especially at the onset of fusion. In this study, the possible positive regulation of myoblast migration by calpains, a crucial step required to align myoblasts to permit them to fuse, was investigated. Inhibition of calpain activity by different pharmacological inhibitors argues for the involvement of these proteinases during the migration of myoblasts. Moreover, a clonal cell line that fourfold overexpresses calpastatin, the endogenous inhibitor of calpains, and that exhibits deficient calpain activities was obtained. The results showed that the migratory capacity of C2C12 and fusion into multinucleated myotubes were completely prevented in these clonal cells. Calpastatin-overexpressing myoblasts unable to migrate were characterized by rounded morphology, the loss of membrane extensions, the disorganization of stress fibers and exhibited a major defect in new adhesion formation. Surprisingly, the proteolytic patterns of desmin, talin, vinculin, focal adhesion kinase (FAK) and ezrin, radixin, moesin (ERM) proteins are the same in calpastatin-overexpressing myoblasts as compared to control cells. However, an important accumulation of myristoylated alanine-rich C kinase substrate (MARCKS) was observed in cells showing a reduced calpain activity, suggesting that the proteolysis of this actin-binding protein is calpain-dependent and could be involved in both myoblast adhesion and migration.


Subject(s)
Calpain/antagonists & inhibitors , Cell Movement/physiology , Intracellular Signaling Peptides and Proteins , Membrane Proteins , Myoblasts/physiology , Animals , Calcium-Binding Proteins/metabolism , Calpain/drug effects , Calpain/metabolism , Cell Adhesion , Cell Fusion , Cell Line , Cell Movement/drug effects , Clone Cells , Cysteine Proteinase Inhibitors/pharmacology , Cytoskeleton/metabolism , Dipeptides/pharmacology , Dose-Response Relationship, Drug , Glucosidases , Leupeptins/pharmacology , Mice , Muscle Fibers, Skeletal/metabolism , Myoblasts/cytology , Myoblasts/drug effects , Myristoylated Alanine-Rich C Kinase Substrate , Oligopeptides/pharmacology , Phosphoproteins/metabolism , Stress Fibers/drug effects , Stress Fibers/metabolism
16.
J Biol Chem ; 278(14): 12443-51, 2003 Apr 04.
Article in English | MEDLINE | ID: mdl-12529328

ABSTRACT

We investigated the status and the regulation of the cyclin-dependent kinases (CDK) inhibitor p27(Kip1) in a choroidal melanoma tumor-derived cell line (OCM-1). By contrast to normal choroidal melanocytes, the expression level of p27(Kip1) was low in these cells and the mitogen-activated protein (MAP) kinase pathway was constitutively activated. Genetic or chemical inhibition of this pathway induced p27(Kip1) accumulation, whereas MAP kinase reactivation triggered a down-regulation of p27(Kip1) that could be partially reversed by calpain inhibitors. In good accordance, ectopic expression of the cellular calpain inhibitor calpastatin led to an increase of endogenous p27(Kip1) expression. In vitro, p27(Kip1) was degraded by calpains, and OCM-1 cell extracts contained a calcium-dependent p27(Kip1) degradation activity. MAP kinase inhibition partially inhibited both calpain activity and calcium-dependent p27(Kip1) degradation by cellular extracts. Immunofluorescence labeling and subcellular fractionation revealed that p27(Kip1) was in part localized in the cytoplasmic compartment of OCM-1 cells but not of melanocytes, and accumulated into the nucleus upon MAP kinase inhibition. MAP kinase activation triggered a cytoplasmic translocation of the protein, as well as a change in its phosphorylation status. This CRM-1-dependent cytoplasmic translocation was necessary for MAP kinase- and calpain-dependent degradation. Taken together, these data suggest that in tumor-derived cells, p27(Kip1) could be degraded by calpains through a MAP kinase-dependent process, and that abnormal cytoplasmic localization of the protein, probably linked to modifications of its phosphorylation state, could be involved in this alternative mechanism of degradation.


Subject(s)
Calpain/metabolism , Cell Cycle Proteins/metabolism , Choroid Neoplasms , MAP Kinase Signaling System/physiology , Melanoma , Tumor Suppressor Proteins/metabolism , Cell Division/physiology , Cyclin-Dependent Kinase Inhibitor p27 , Humans , In Vitro Techniques , Melanocytes/cytology , Melanocytes/enzymology , Mitogen-Activated Protein Kinase 1/metabolism , Mitogen-Activated Protein Kinase 3 , Mitogen-Activated Protein Kinases/metabolism , Phosphorylation , Substrate Specificity , Tumor Cells, Cultured
17.
Behav Brain Res ; 136(2): 439-48, 2002 Nov 15.
Article in English | MEDLINE | ID: mdl-12429406

ABSTRACT

Several behavioral and electrophysiological studies have suggested that a sustained activation of protein kinase C would be required to underlie persistent changes associated with memory formation. Limited proteolysis of PKCs by calpains, calcium-activated proteases, cleaves the catalytic and the regulatory domains, generating a free catalytic fragment termed PKM, constitutively active. In order to investigate the potential physiological importance of this limited proteolysis as a mechanism of PKC activation, we have studied the effect of the calpastatin peptide, a specific calpain inhibitor, on the learning of a spatial discrimination task in a radial maze. Thus, using osmotic micro-pumps, the calpastatin peptide was infused bilaterally into the dorsal hippocampus during the six sessions of training and the probe test. The treatment was shown to facilitate the performance of the mice on the two last training sessions and on the probe test. This behavioral effect was shown to correspond to the reduced calpain activity observed in the hippocampus at the very end of the 7-day infusion of the calpastatin peptide, suggesting a relation between both events. In addition, PKC activity measured immediately after the probe test was notably decreased in the membrane fraction of the hippocampus. Although protein levels of PKCs and calpains quantified by western blot were not affected by calpastatin infusion, we found a noticeable correlation between mu-calpain and PKCgamma levels confirming the particular relationship between both proteins. These results suggest that calpains influence on PKCs activity may affect cellular mechanisms during memory processes.


Subject(s)
Calpain/antagonists & inhibitors , Cysteine Proteinase Inhibitors/pharmacology , Discrimination Learning/drug effects , Hippocampus/enzymology , Protein Kinase C/metabolism , Space Perception/drug effects , Animals , Calcium-Binding Proteins/administration & dosage , Calcium-Binding Proteins/pharmacology , Calpain/metabolism , Cell Membrane/enzymology , Cell Membrane/metabolism , Cysteine Proteinase Inhibitors/administration & dosage , Cytosol/enzymology , Cytosol/metabolism , Drug Implants , Habituation, Psychophysiologic/physiology , Hippocampus/drug effects , Immunoblotting , Injections, Intraventricular , Male , Maze Learning/drug effects , Mice , Mice, Inbred C57BL , Nerve Tissue Proteins/metabolism
18.
Biol Cell ; 94(2): 65-76, 2002 May.
Article in English | MEDLINE | ID: mdl-12148243

ABSTRACT

Previous studies have led us to hypothesize that m-calpain plays a pivotal role in myoblast fusion through its involvement in cell membrane and cytoskeleton component reorganization. To support this hypothesis, a convenient and simple myoblast culture model using frozen embryonic myoblasts was developed, which resolved a number of problems inherent to cell primary culture. Biological assays on cultured myoblasts using different media to define the characteristics of the fusion process were first conducted. Proteinase was detectable before the initiation of the fusion process and was closely correlated to the phenomenon of fusion under each culture condition studied. In addition, the study of calpastatin showed that the initiation of fusion does not require a decrease in the level of this endogenous inhibitor of calpains and also confirmed that calpastatin may be implicated in the determination of the end of fusion. On the other hand, analysis of the evolution of myogenic factors revealed that myogenins, MyoD and Myf5, increase very significantly during the formation of multinucleated myotubes. Moreover, the antisense technique against myogenin is capable of preventing the process of fusion by 50%, confirming the pivotal role of this factor in the early stages of differentiation. The possible role of myogenic regulator factors on m-calpain gene expression is discussed.


Subject(s)
Calpain/physiology , Models, Biological , Muscle Development/physiology , Myoblasts/physiology , Animals , Calcium-Binding Proteins/metabolism , Cell Fusion , Cells, Cultured , Myogenin/antagonists & inhibitors , Rats , Rats, Wistar
19.
J Biotechnol ; 96(3): 271-9, 2002 Jul 03.
Article in English | MEDLINE | ID: mdl-12044555

ABSTRACT

p94 belongs to the calpain family of enzymes, also called calcium-activated neutral proteases and is mainly expressed in the skeletal muscle. Mutations affecting the gene coding for p94 are responsible for a myopathy syndrome called Limb Girdle Muscular Dystrophy type 2A (LGMD2A). Although the activity of p94 seems necessary for muscle function, the biological role of the enzyme is still unknown. The goal of this study was to develop a muscle cell line in which the expression level of p94 can be regulated, by an inducible way. In this study, a biological system was developed which allowed mimicking, in vitro, of part of the events occurring in patients (i.e. a decrease of p94 activity). The first results indicate that the decrease in p94 activity results in a significant increase of myogenin level, a high specific transcription factor involved in myoblast fusion. This muscle specific inducible system is an interesting biological tool to assess specifically p94 function(s) in cultured muscle cells. According to the present results, p94 seems at least to be involved in a myogenesis regulation pathway via its action on certain proteins belonging to the myogenic regulator factor family.


Subject(s)
Calpain/metabolism , Muscle Development/physiology , Muscle, Skeletal/enzymology , Myogenin/metabolism , Animals , Blotting, Western , Calpain/genetics , Cell Culture Techniques/methods , Gene Expression/physiology , Gene Expression Regulation , Isopropyl Thiogalactoside/metabolism , Mice , Muscle Development/genetics , Muscle, Skeletal/cytology , Nucleic Acid Amplification Techniques , RNA, Antisense/genetics , RNA, Messenger/genetics
20.
Mol Cell Biochem ; 231(1-2): 147-54, 2002 Feb.
Article in English | MEDLINE | ID: mdl-11952156

ABSTRACT

Many studies have demonstrated that the calcium-dependent proteolytic system (calpains and calpastatin) is involved in myoblast differentiation. It is also known that myogenic differentiation can be studied in vitro. In the present experiments, using a mouse muscle cell line (C2C12) we have analyzed both the sequences of appearance and the expression profiles of calpains 1, 2, 3 and calpastatin during the course of myoblast differentiation. Our results mainly show that the expression of ubiquitous calpains (calpain 1 and 2) and muscle-specific calpain (calpain 3) at the mRNAs level as well as at the protein level do not change significantly all along this biological process. In the same time, the specific inhibitor of ubiquitous calpains, calpastatin, presents a stable expression at mRNAs level as well as protein level, all along myoblast to myotube transition. A comparison with other myogenic cells is presented.


Subject(s)
Calcium-Binding Proteins/metabolism , Calcium/metabolism , Calpain/metabolism , Muscles/metabolism , Animals , Calcium-Binding Proteins/genetics , Calpain/genetics , Cell Differentiation , Cell Fusion , Cell Line , Gene Expression Regulation, Enzymologic , Mice , Muscle Development , Muscles/cytology , Muscles/enzymology , RNA, Messenger/genetics , RNA, Messenger/metabolism , Reverse Transcriptase Polymerase Chain Reaction , Time Factors
SELECTION OF CITATIONS
SEARCH DETAIL
...