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1.
Ann N Y Acad Sci ; 1130: 179-87, 2008.
Article in English | MEDLINE | ID: mdl-18596347

ABSTRACT

Fluorescence probes and labels have become indispensable tools for clinical diagnostics, high-throughput screening, and other biomedical applications. We have developed several classes of new squaraine-based red and near-infrared (NIR) probes and labels (SETA and Square series), naphthalimide-based fluorescence lifetime dyes (SeTau series), and cyanine- and squaraine-based quenchers (SQ series). This report discusses the spectral and photophysical properties of these new markers. In particular, the red and NIR dyes of the SETA and Square series are extremely bright, with photostabilities that are unmatched by any other dyes in the same spectral region.


Subject(s)
Cyclobutanes/chemistry , Fluorescent Dyes/pharmacology , Phenols/chemistry , Spectroscopy, Near-Infrared/methods , Animals , Cattle , Humans , Hydrogen-Ion Concentration , Immunoglobulin G/chemistry , Light , Models, Chemical , Photochemistry/methods , Saccharomyces cerevisiae/metabolism , Serum Albumin/chemistry , Spectrophotometry/methods , Time Factors
2.
Anal Chim Acta ; 570(2): 214-23, 2006 Jun 16.
Article in English | MEDLINE | ID: mdl-17723402

ABSTRACT

A series of ring-substituted squaraines absorbing and emitting in the red and NIR spectral region was synthesized and their spectral and photophysical properties (quantum yields, fluorescence lifetimes) and photostabilities were measured and compared to Cy5, a commonly used fluorescent label. The absorption maxima in aqueous media were found to be between 628 and 667 nm and the emission maxima are between 642 and 685 nm. Squaraine dyes exhibit high extinction coefficients (163,000-265,000 M(-1) cm(-1)) and lower quantum yields (2-7%) in aqueous buffer but high quantum yields (up to 45%) and long fluorescence lifetimes (up to 3.3 ns) in presence of BSA. Dicyanomethylene- and thio-substituted squaraines exhibit an additional absorption around 400 nm with extinction coefficients between 21,500 and 44,500 M(-1) cm(-1). These dyes are excitable not only with red but also with blue diode lasers or light emitting diodes. Due to the favourable spectral and photophysical properties these dyes can be used as fluorescent probes and labels for intensity- and fluorescence lifetime-based biomedical applications.

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