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Methods Mol Biol
; 2232: 113-122, 2021.
Article
in English
| MEDLINE
| ID: mdl-33161543
ABSTRACT
Assessment of endophytic and saprotrophic microbial communities from wood-extracted DNA presents challenges due to the presence of surface microbes that contaminate samples and plant compounds that act as inhibiting agents. Here, we describe a method for decontaminating, sampling, and processing wood at various stages of decay for high-throughput extraction and purification of DNA.