[Activity of proteolytic and nucleolytic enzymes from the gonades of hydrobionts]. / Aktivnost' proteoliticheskikh i nukleoliticheskikh fermentov v gonadakh gidrobiontov.

The activity of nucleolytic and proteolytic enzymes in milt of nine kinds of fishes belonging to various families and of three kinds invertebrates is determined. There is carried out electrophoreses division of preparations DNA, received from milts by various methods; there are determined structure and molecular weights of oligonucleotides. The influence of activity tissue enzymes on a destruction degree of DNA is established at addition enzymes of exogenic origin.

[Effect of endogenous enzymes on composition of oligonucleotides isolated from gonads of hydrobionts]. / Vliianie éndogennykh fermentov na sostav ologonucleotidov pri ikh vydelenii iz gonad gidrobiontov.

Preparations of low-molecular-weight DNA were obtained from gonads of various hydrobiont species by alcohol precipitation technique. An enzymatic hydrolysis-based method for producing soluble low-molecular-weight DNA was developed. All the nucleic acid components of the preparations obtained were separated by electrophoresis, and their molecular weights were determined. It was demonstrated that high activities of endogenous enzymes (nucleases and proteases) in milts of hydrobionts correlated with the degree of hydrolysis of their nucleic acids.

[Enzymatic methods of preparation of protein hydrolysates by using proteolytic preparations of varying specificity]. / Fermentativnye sposoby prigotovleniia belkovykh gidrolizatov s ispol'zovaniem proteoliticheskikh preparatov razlichnoi spetsifichnosti.

The optimal conditions of enzymatic hydrolysis of proteins different composition and structure (protamin, casein, haemoglobin, fibrin, collagen) were established by using proteolytic enzymatic preparations with different specificity (pancreatin, pilrin, hepatopancreatin). The degree of hydrolysis, molecular and amino acid compositions of proteins were determined.