ABSTRACT
Rat brain synaptosomes were isolated to study the effects of protein kinase inhibitors (sphingosine, 1-(5-isoquinolinesulfonyl)-2-methylpiperazine dihydrochloride, N-(6-aminohexyl)-5-chloro-1-naphtalenesulfonamide, staurosporine) on Ca2+-dependent and Ca2+-independent [14C]GABA release. The Ca2+-dependent [14C]GABA release was stimulated by depolarization with a K+-channel blocker, 4-aminopyridine, or high K+ concentration. It has been shown that 4-aminopyridine-evoked [14C]GABA release strongly depends on extracellular Ca2+ while K+-evoked [14C]GABA release only partly decreases in the absence of calcium. The substitution of sodium by choline in Ca2+-free medium completely abolished Ca2+-independent part of K+-evoked [14C]GABA release. So the main effect of 4-aminopyridine is the Ca2+-dependent one while high K+ is able to evoke [14C]GABA release in both a Ca2+-dependent and Na+-dependent manner. In experiments with protein kinase inhibitors, 4-aminopyridine and high K+ concentration were used to study the Ca2+-dependent and the Ca2+-independent [14C]GABA release, respectively. In addition, the Ca2+-independent [14C]GABA release was studied using alpha-latrotoxin as a tool. Pretreatment of synaptosomes with protein kinase inhibitors tested, except of 1-(5-isoquinolinesulfonyl)-2-methylpiperazine dihydrochloride, resulted in a marked inhibition of 4-aminopyridine-stimulated Ca2+-dependent [14C]GABA release. The inhibitory effects of N-(6-aminohexyl)-5-chloro-1-naphtalenesulfonamide and staurosporine on [14C]GABA release were not due to their effects on 4-aminopyridine-promoted 45Ca2+ influx into synaptosomes. Only sphingosine (100 microM) reduced the 45Ca2+ influx. All the inhibitors investigated were absolutely ineffective in blocking the Ca2+-independent [14C]GABA release stimulated by alpha-latrotoxin. Three of them, except for sphingosine, did not affect the Ca2+-independent [14C]GABA release stimulated by high potassium. The inhibitory effect of sphingosine was equal to 30%. Thus, if [14C]GABA release occurred in a Ca2+-independent manner irrespective of whether alpha-latrotoxin or high K+ stimulated this process, it was not inhibited by the drugs decreased the Ca2+-dependent [14C] GABA release. Given the above points it is therefore not unreasonable to assume that the absence of Ca2+ in the extracellular medium created the conditions in which the activation of neurotransmitter release was not accompanied by Ca2+-dependent dephosphorylation of neuronal phosphoproteins, and as a consequence the regulation of exocytotic process was modulated so that the inhibition of protein kinases did not disturb the exocytosis.
Subject(s)
Brain Chemistry/drug effects , Enzyme Inhibitors/pharmacology , Synaptosomes/enzymology , gamma-Aminobutyric Acid/pharmacokinetics , 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine/pharmacology , 4-Aminopyridine/pharmacology , Animals , Biological Transport/drug effects , Calcium/metabolism , Carbon Radioisotopes , Male , Potassium Chloride/pharmacology , Rats , Sphingosine/pharmacology , Spider Venoms/pharmacology , Staurosporine/pharmacology , Sulfonamides/pharmacology , Synaptosomes/drug effectsABSTRACT
We have studied [14C]GABA release from synaptosomes induced by native and monoclonal antibodies-modified alpha-latrotoxin (LTX). Modification of LTX eliminates the toxin's ability to increase [Ca2+]i influx into synaptosomes. It has been shown that native LTX does not change 22Na influx into rat brain synaptosomes. Both toxin forms studied, native and modified by monoclonal antibodies, stimulate [14C]GABA release from synaptosomes in divalent-free medium where sodium was substituted by equimolar concentrations of choline chloride. Native toxin induces a more rapid stimulation of [14C]GABA release than the modified one. It was suggested that the difference in the mediator release rates is not accounted for by the inability of modified toxin to form active ion channels in synaptosomal plasmalemma, but most probably by the state of toxin-receptor complexes.
Subject(s)
Brain/metabolism , Spider Venoms/pharmacology , Synaptosomes/metabolism , gamma-Aminobutyric Acid/metabolism , Animals , Antibodies, Monoclonal/drug effects , Antigen-Antibody Complex , Biological Transport , Brain/drug effects , Calcium/metabolism , Male , Rats , Sodium/metabolism , Spider Venoms/immunology , Synaptosomes/drug effectsABSTRACT
We have studied the action of an alpha-latrotoxin (alpha-LTX) complex of two polypeptides (LTX 130 kDa and low molecular weight protein (LMWP) 8 kDa) and the action of a venom fraction containing LTX with excess LMWP on calcium influx into synaptosomes and PC12 cells as well as on [14C]GABA release from synaptosomes. Both preparations considerably activate calcium influx and stimulate [14C]GABA release from synaptosomes. Preincubation of both preparations with antibodies against a 14 amino acid residue C-terminal peptide of LMWP differentially modulates these effects. Antibodies inhibit induced calcium influx and enhance induced GABA release.