ABSTRACT
The experimental preclinical study on mice showed that a complex of polyhydroxylated naphthoquinone pigments and minerals from shells of sea urchins decreases the concentration of glucose, stimulates the synthesis of phospholipids in liver, and has antioxidant properties. On the model of streptozotocin-nicotinamide-induced type II diabetes, this complex of polyhydroxylated naphthoquinone pigments and minerals from sea urchin shells exhibited activity after administration for 10 days in a dose of 1.8 mg/kg.
Subject(s)
Blood Glucose/metabolism , Diabetes Mellitus, Experimental , Diabetes Mellitus, Type 2 , Hypoglycemic Agents/pharmacology , Oxidative Stress/drug effects , Pigments, Biological/pharmacology , Sea Urchins/chemistry , Animal Structures/chemistry , Animals , Diabetes Mellitus, Experimental/blood , Diabetes Mellitus, Experimental/drug therapy , Diabetes Mellitus, Type 2/blood , Diabetes Mellitus, Type 2/drug therapy , Hypoglycemic Agents/chemistry , Male , Mice , Pigments, Biological/chemistry , Time FactorsABSTRACT
A simple, specific and sensitive RP-HPLC method with UV detection for the determination of hopantenic acid in human blood plasma has been developed. The pharmacokinetics of drug pantocalcin upon single peroral administration was investigated on 18 healthy volunteers. The peak of hopantenic acid in blood plasma was achieved at 1.56 h and the elimination half life was 6.68 h. No hopantenic acid in blood plasma is found in 48 h.
Subject(s)
Nootropic Agents/administration & dosage , Nootropic Agents/pharmacokinetics , Pantothenic Acid/analogs & derivatives , gamma-Aminobutyric Acid/analogs & derivatives , Administration, Oral , Adult , Chromatography, High Pressure Liquid , Female , Half-Life , Humans , Male , Pantothenic Acid/administration & dosage , Pantothenic Acid/pharmacokinetics , gamma-Aminobutyric Acid/administration & dosage , gamma-Aminobutyric Acid/pharmacokineticsABSTRACT
Results of phytochemical analysis of the berries from six Russian Hippophae rhamnnoides L. varieties (Augustinka, Botanitseskaja, Ljubitelskaja, Vorobjvskaja, Pertsik, Trofimovskaja), cultivated in Finland, are presented. The contents of ascorbic acid (vitamin C), tocopherols (vitamin E), total flavonoid (vitamin P), total carotenoid (vitamin A) and total organic acids were analyzed in unfreezed berries. The same parameters were defined in food products (jelly, sweet sauce and compote) containing fruits of three Hippophae rhamnoides L. varieties (Botanitseskaja, Ljubitelskaja and Trofimovskaja). According to the results, Russian Hippophae rhamnoides L. varieties can be cultivated in South-Finland and different kinds of food products with high quality and concentration of biologically active compounds may be prepared from these berries.
Subject(s)
Antioxidants/analysis , Food Analysis/methods , Fruit , Hippophae , Ascorbic Acid/analysis , Carotenoids/analysis , Finland , Flavonoids/analysis , Food, Preserved/analysis , Fruit/chemistry , Fruit/classification , Fruit/growth & development , Hippophae/chemistry , Hippophae/classification , Hippophae/growth & development , Russia , Tocopherols/analysisABSTRACT
Light-absorbing and antiradical properties of the new product on a basis of lutein and zeaxanthin for correction of eye diseases in model system of initiated oxidation of isopropylbenzene were investigated. It is shown, that the product is the effective light-absorbing agent and inhibitor of free-radical oxidation in vitro. In experiments on animals (rat) the pharmacokinetics of the product was investigated at single oral administration. A simple, specific and sensitive RP-HPLC method for the determination of lutein in rat plasma was developed, which was applied to pharmacokinetic investigation in rats after oral administration of lutein at dose 20 mg/kg. It was established, that the peak plasma levels was achieved to 2 hour and the mean elimination half life was 2,4 hours.
