ABSTRACT
The capacity of mouse bone marrow cells to adhere to calcium phosphate surfaces and form tissue plates depending on the surface relief and solubility was studied in ectopic bone formation test. Calcium phosphate coating of titanium disks, made by the anodic spark (microarch) oxidation in 10% orthophosphoric acid with hydroxyl apatite particles, differed by the structure (thickness of coating, size of pores, and roughness) and solubility (level of in vitro oxidation of 1-week extracts of implants). Chemical (phasic and element) composition of the studied calcium phosphate coatings was virtually the same. The findings indicate that histogenesis is regulated by physicochemical characteristics of the implant surface. It seems that the osteogenic potential of calcium phosphate surfaces is largely determined by their relief, but not by pH of degradation products.
Subject(s)
Bone Marrow Cells/drug effects , Calcium Phosphates/pharmacology , Cell Adhesion , Coated Materials, Biocompatible/pharmacology , Osteogenesis , Animals , Bone Marrow Cells/physiology , Bone and Bones/physiology , Bone and Bones/ultrastructure , Calcium Phosphates/chemistry , Cell Adhesion/drug effects , Coated Materials, Biocompatible/chemistry , Hydrogen-Ion Concentration , Hydroxyapatites/chemistry , Hydroxyapatites/pharmacology , Implants, Experimental , Mice , Solubility , Surface Properties , Titanium/chemistryABSTRACT
Biological activity of eight hydroxyapatite powders synthesized by the mechanochemical method was studied in vitro. Introduction of copper and zinc atoms into synthetic hydroxyapatite structure promotes cell adhesion. Hydroxyapatites obtained by the mechanochemical method are promising base for a new class of materials and coatings with regulated bioactivity for orthopedics and traumatology.
Subject(s)
Durapatite/chemical synthesis , Durapatite/pharmacology , Megakaryocytes/drug effects , Animals , Cell Adhesion/drug effects , Cells, Cultured , Copper/chemistry , Copper/pharmacology , Durapatite/chemistry , Male , Megakaryocytes/physiology , Rats , Zinc/chemistry , Zinc/pharmacologyABSTRACT
Specific biological activity of the domestic preparation of recombinant human erythropoietin (RHEPO) in an original pill form was experimentally studied in vitro and in vivo. Administration of the RHEPO parent substance at a concentration of 0.5 10 U/ml significantly accelerated the growth of erythroid colony-forming cells (CFU-E) in the culture of intact nonadherent myelokaryocytes. The erythropoiesis-stimulating properties of the new RHEPO preparation are comparable with those of the well-known injection preparations. The drug action is related to increasing the erythroid cell content in the bone marrow and the reticulocyte and erythrocyte counts in the peripheral blood.
Subject(s)
Erythropoietin/pharmacology , Hematopoiesis/drug effects , Administration, Oral , Animals , Blood Cell Count , Cell Line , Colony-Forming Units Assay , Epoetin Alfa , Erythropoiesis/drug effects , Erythropoietin/administration & dosage , Humans , Male , Mice , Mice, Inbred CBA , Recombinant Proteins/administration & dosage , Recombinant Proteins/pharmacology , Reticulocytes/drug effects , TabletsABSTRACT
The effects of anticancer drugs whose action is mediated by different mechanisms on activity of bone marrow fibroblasts and their role in the regulation of granulocytopoiesis recovery after cytostatic treatment were studied. The antimetabolite 5-fluorouracil strongly suppressed, while the anthracycline antibiotic adriamycin and the alkylating agent cyclophosphamide stimulated function of stromal cells. This largely accounted for different dynamics of recovery of the bone marrow neutrophil lineage.
Subject(s)
Antineoplastic Agents/pharmacology , Bone Marrow Cells/pathology , Fibroblasts/pathology , Granulocytes/pathology , Animals , Bone Marrow Cells/physiology , Cell Differentiation , Fibroblasts/physiology , Granulocytes/physiology , Hematopoiesis , Male , Mice , Mice, Inbred CBAABSTRACT
We compared hemopoiesis-stimulating activities of dry pantohematogen and recombinant granulocyte colony-stimulating factor under conditions of cyclophosphamide-induced myelosuppression. These preparations stimulated regeneration of bone marrow granulomonocytopoiesis by various mechanisms.
Subject(s)
Allergens/pharmacology , Antineoplastic Agents, Alkylating/pharmacology , Biological Factors , Cyclophosphamide/pharmacology , Granulocyte Colony-Stimulating Factor/pharmacology , Leukopoiesis/drug effects , Animals , Granulocytes/drug effects , Mice , Mice, Inbred CBA , Monocytes/drug effects , Recombinant ProteinsABSTRACT
Pantohematogen stimulates hemopoiesis in mice with cyclophosphane induced bone marrow hypoplasia. The hemopoiesis recovery activated by pantohematogen is manifested by the granulocyte lineage hyperplasia in the bone marrow, rapid restoration of the polymorphonuclear leukocyte and monocyte counts in the peripheral blood, and the development of neutrophilia and monocytosis. The activation effect of pantohematogen on the bone marrow hemopoiesis is related to stimulating the precursor cell proliferation and differentiation and to increasing the functional activity of the hemopoietic microenvironment.
