Correlation of C-Reactive Protein and Severity of Peri-Implant Diseases.

Peri-implant disease pathogenesis is similar to periodontal disease pathogenesis resulting in production of pro-inflammatory mediators. These mediators are released during the inflammation phase, among which C-reactive protein (CRP) is one of the acute phase reactants. The aim of the study was to correlate the levels of CRP with the severity of peri-implant diseases. The present observational study was carried out from June 2022 to December 2022 in the Department of Implantology, Saveetha Dental College and Hospitals, Chennai, India. A total of 60 patients with peri-implant health (n = 20), peri-mucositis (n = 20) and peri-implantitis (n = 20) were enrolled. Unstimulated salivary samples were collected and subjected to latex agglutination assay for CRP analysis. CRP levels were then correlated with severity of peri-implant diseases. The mean CRP level in peri-implant health, peri-implant mucositis, peri-implantitis were 0.25 ± 0.36 mg/dl, 3.56 ± 0.85 mg/dl and 5.07 ± 0.74 mg/dl, respectively. Pearson correlation coefficient analysis revealed a strong positive correlation between CRP and peri-implant parameters suggesting that the CRP level increased as the severity of peri-implant disease increased. CRP level increases with severity of peri-implant diseases and there exists a positive correlation between CRP level and peri-implant parameters. Therefore, CRP can be used as a diagnostic marker for peri-implant diseases.

Amylase-producing marine actinobacterium of Micromonospora sp. and their potential antibacterial effects against oral pathogens.

Marine actinobacteriological investigation is still in its beginning in India. Earlier, in the 20th century, studies on Actinobacteria were started and highly concentrated on diversity, identification, and screening for enzymes, antibiotics, and enzyme inhibitors. With the spurge of infectious diseases requiring antibiotics, novel antibiotics are in search as the prevalent ones have declined uses, due to the antibiotic-resistant microbial growth. Unexploited ecosystems are studied for isolation of rare species such as Actinobacteria which are expected to yield newer metabolites. The marine actinobacterial isolation and enumeration were done from sediment samples. The marine Actinobacteria were identified by conventional methods. Further amylase enzyme production and their antibacterial activities are also done following the standard methods. The Micromonospora sp. was identified by chemotaxonomical characteristics and spore chain morphology. Further, the amylase enzyme production was done and quantification of enzyme also done. The potential antimicrobial activity from the amylase enzyme was done. Zone of inhibition and minimal inhibitory concentration were calculated. It concluded that potent antibacterial activity was obtained from Actinobacteria Micromonospora sp. producing amylase enzymes.