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1.
Langmuir ; 2024 May 29.
Article in English | MEDLINE | ID: mdl-38809114

ABSTRACT

In the present study, we report the evolution of stabilizing Pickering emulsions using brush-modified Janus particles (JPs), utilizing the dissipative particle dynamics (DPD) simulation technique. Our results are subsequently corroborated with experimental findings. Each JP has one-half of the hydrophobic surface, with the other half embedded with hydrophilic polymer brushes grown via atom transfer radical polymerization (ATRP). Our generic simulation model analyzes the chemical kinetics of polymer brush growth on one-half of the initiator-embedded microparticle (MP) surface, resulting in the formation of JP. This involves evaluating monomer conversion and reaction rates. Our results exhibit a substantial influence of the number of JPs, grafted brush density, and brush length on oil-in-water emulsion stability. We studied the evolution kinetics and stability of emulsion formation by analyzing the growth of average domain size and corresponding scaling functions up to a late time limit. This study aims to clarify the connection between the size, quantity, and functionality of JPs and the stability of Pickering emulsions.

2.
J Mater Chem B ; 11(16): 3617-3634, 2023 04 26.
Article in English | MEDLINE | ID: mdl-37014090

ABSTRACT

To date, the synthesis of efficient and safe gene carriers with low toxicity and appreciable gene transfection efficiency has been the major hurdle associated with non-viral gene carriers. Herein, we synthesized three amino acid-based diblock copolymers comprising glycine-leucine, leucine-phenyl alanine and glycine-phenyl alanine group containing blocks. The synthesis of all the diblock copolymers was confirmed by FTIR, 1H NMR, DLS and GPC techniques. All the polymers showed a high positive zeta potential value that varies from 45 ± 1 mV to 56 ± 1 mV, and the hydrodynamic size of the polymers varies from 250 ± 8 to 303 ± 14 nm. The three polymers showed negligible cytotoxicity compared with PEI (25 kDa) for MDA-MB-231 and NKE cells. Among all other polymers, P(HGN)n-b-P(HPN)m exhibited the highest biocompatibility with ∼70% cell viability at a concentration of 200 µg mL-1. Hemolysis data revealed that among all three polymers, P(HGN)n-b-P(HPN)m exhibited the highest blood compatibility, while up to a high concentration of 200 µg mL-1, it showed a very negligible amount (∼18%) of hemolysis. Most importantly, excellent gene complexation capability and good protection of pDNA against enzymatic degradation were observed with all three diblock copolymers. Interestingly, P(HGN)n-b-P(HPN)m/pDNA complex showed the smallest particle size (∼15 nm) and highest positive zeta potential as observed from TEM micrographs and DLS analysis, which probably results significantly higher level of cellular uptake and hence the highest transfection efficiency (∼85%) against MDA-MB-231 cells. Therefore, the diblock copolymer P(HGN)n-b-P(HPN)m with superior gene transfection efficiency in triple negative breast cancer may be an efficient non-viral vector for successful TNBC therapy in the future.


Subject(s)
Polyethylene Glycols , Triple Negative Breast Neoplasms , Humans , Polyethylene Glycols/chemistry , Triple Negative Breast Neoplasms/genetics , Amino Acids , Leucine , Hemolysis , Polymers/chemistry , Transfection , Glycine , Alanine
3.
Adv Colloid Interface Sci ; 300: 102580, 2022 Feb.
Article in English | MEDLINE | ID: mdl-34922246

ABSTRACT

Current advancements in the creation of anisotropy in particles and their surface modification with polymer brushes have established a new class of hybrid materials termed polymer brush modified anisotropic particles (PBMAP). PBMAPs display unique property combinations, e.g., multi-functionality in multiple directions along with smart behavior, which is not easily achievable in traditional hybrid materials. Typically, anisotropic particles can be categorized based on three different factors, such as shape anisotropy (geometry driven), compositional anisotropy (functionality driven), and surface anisotropy (spatio-selective surface modification driven). In this review, we have particularly focused on the synthetic strategies to construct the various type of PBMAPs based on inorganic or organic core which may or may not be isotropic in nature, and their applications in various fields ranging from drug delivery to catalysis. In addition, superior performances and fascinating properties of PBMAPs over their isotropic analogues are also highlighted. A brief overview of their future developments and associated challenges have been discussed at the end.


Subject(s)
Drug Delivery Systems , Polymers , Anisotropy
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