Parasitic wasps do not lack lipogenesis.

Fatty acids are crucial primary metabolites for virtually all creatures on earth. Most organisms thus do not rely exclusively on a nutritional supply containing fatty acids, but have the ability to synthesize fatty acids and triacylglycerides de novo from carbohydrates in a process called lipogenesis. The ubiquity of lipogenesis has been questioned by a series of studies reporting that many parasitic wasps (parasitoids) do not accumulate lipid mass despite having unlimited access to sugar. This has been interpreted as an evolutionary metabolic trait loss in parasitoids. Here, we demonstrate de novo biosynthesis of fatty acids from 13C-labelled α-d-glucose in 13 species of parasitoids from seven families. We furthermore show in the model organism Nasonia vitripennis that lipogenesis occurs even when lipid reserves are still intact, but relative 13C-incorporation rates increase in females with widely depleted fat reserves. We therefore conclude that the presumed 'lack of lipogenesis' in parasitoids needs to be re-evaluated.

De novo biosynthesis of linoleic acid is widespread in parasitic wasps.

Linoleic acid (C18:2∆9,12 , LA) is an important metabolite with numerous essential functions for growth, health, and reproduction of organisms. It has long been assumed that animals lack ∆12-desaturases, the enzymes needed to produce LA from oleic acid (C18:1∆9 , OA). There is, however, increasing evidence that this is not generally true for invertebrates. In the insect order Hymenoptera, LA biosynthesis has been shown for only two parasitic wasp species of the so-called "Nasonia group," but it is unknown whether members of other taxa are also capable of synthesizing LA. Here, we demonstrate LA biosynthesis in 13 out of 14 species from six families of parasitic wasps by gas chromatography-mass spectrometry analysis using two different stable isotope labeling techniques. Females of the studied species converted topically applied fully 13 C-labeled OA into LA and/or produced labeled LA after feeding on fully 13 C-labeled α- d-glucose. These results indicate that ∆12-desaturases are widespread in parasitic Hymenoptera and confirm previous studies demonstrating that these insects are capable of synthesizing fatty acids de novo.

De novo biosynthesis of fatty acids from α-D-glucose in parasitoid wasps of the Nasonia group.

Fatty acids are indispensable primary metabolites for virtually any organism on earth and thus enzymatic machinery enabling de novo production of fatty acids from carbohydrates is highly conserved. A series of studies has questioned the ubiquity of lipogenesis in parasitoid wasps suggesting that the vast majority of species have lost the ability to synthesize fatty acids de novo. One such species is Nasonia vitripennis, which, like the congeneric species N. giraulti and N. longicornis, uses a fatty acid-derived male sex pheromone for sexual communication. Here we demonstrate by feeding fully 13C-labeled α-D-glucose and analyzing insect-derived fatty acid methyl esters and the male sex pheromone by coupled gas chromatography/mass spectrometry that both males and females of N. vitripennis as well as N. giraulti and N. longicornis are capable of synthesizing fatty acids de novo. We furthermore show by a proteomics approach that predicted fatty acid synthase, ATP-citrate synthase, and acetyl-CoA carboxylase, key enzymes of lipogenesis, are expressed in the male pheromone gland of N. vitripennis and N. giraulti. Labeling experiments with Urolepis rufipes, a closely related species producing a male sex pheromone independently of fatty acids via the mevalonate pathway, revealed that both sexes are likewise able to synthesize fatty acids de novo. We conclude that the parasitoid wasp species studied here, irrespective of the biosynthetic origin of their sex pheromones, are capable of responding flexibly to lipid shortage during their adult life by keeping enzymatic machinery for lipogenesis running.