ABSTRACT
Heterotrophic protists play a crucial role in plant growth promotion via nutrient cycling and shift in microbial community composition in the soil ecosystem. Selective predation pressure by protists contributes to the evaluation of plant beneficial traits in rhizospheric bacteria. However, not always all plant growth-promoting bacterial (PGPB) strains are benefitted by predation. This study aimed to examine the predatory effect of Acanthamoeba sp genotype T4 on a range of PGPB strains and their combined impact on early rice seedling growth. Acanthamoeba sp isolated from rice rhizosphere soils were used to assess predation against several PGPB such as Pseudomonas, Bacillus, Enterobacter, Morganella, Stenotrophomonas, Providencia, and Lysinibacillus on Nutrient Yeast Extract agar (NYE) plate. The controlled experiment on the germinated rice seeds (Oryza sativa L.) grown in Petri dishes containing each PGPB strain and Acanthamoeba sp was performed to evaluate the combined impact on plant performance. The PGPB-Acanthamoeba combined treatments in Petri dishes showed significant rice seedling growth compared to PGPB alone, non-PGPB and control. Our results indicated the positive but different impact of Acanthamoeba sp with different PGPB species on early rice plant growth. Further in-depth research should be carried out with diverse protists and PGPB species to assess which protist species can be linked to enhancement of indigenous soil PGPB for improved plant growth.
