ABSTRACT
Gamma-aminobutyric acid (GABA) production by free and Ca-alginate encapsulated cells of Enterococcus faecium CFR 3003 was investigated. Mass transfer rates characterizing the GABA production process using encapsulated cells were investigated. Experiments were performed to investigate external film and internal pore diffusion mass transfer rates. The Damkohler and Thiele analysis provides a good description of external film and internal pore diffusion resistances, respectively. The experiments revealed that the external film effects could be neglected but the process is affected to the greater extent by internal mass transfer effects and was found to be the principal rate-controlling step. Protective effect of encapsulation on cell survivability was tested under digestive environment, when challenged to salivary α-amylase, simulated gastric fluid and intestinal fluid. Viability of encapsulated cells was significantly higher under simulated gastro-intestinal conditions and could produce higher GABA than those observed with free cells. The results indicate that the Ca-alginate encapsulated probiotics could effectively be delivered to the colonic site for effective inhibitory action.
