Subject(s)
DNA/pharmacology , Manganese/pharmacology , Thymus Extracts/pharmacology , Animals , Cattle , DNA/administration & dosage , Drug Interactions , In Vitro Techniques , Intercalating Agents , Manganese/administration & dosage , Phosphates/administration & dosage , Phosphates/pharmacology , Thymus Extracts/administration & dosageABSTRACT
The interaction of Cu(II) ions with native and denatured DNA as a function of ionic strength of the solution was studied by the equilibrium dialysis method. Graphical analysis of binding isotherms confirmed the occurrence of interstrand and intrastrand binding of Cu(II) with DNA and made possible determination of the respective binding constants. To facilitate interpretation of the data, a new molecular model of Cu(II)-DNA binding has been proposed, assuming interstrand intercalation of one Cu(II) ion between two GC pairs both in the successive even and odd groups of GC pairs, and interstrand binding of Cu(II) to the isolated GC pairs, with the exception of T-C-T and T-G-T sequences. In agreement with this model, the DNA-Cu(II) complex is most stable under the equilibrium with free Cu(II) ions at 4 degrees C, pH 6 when the molar ratio of GC pairs to Cu(II) ions bound interstrandially attains GC/Cuinter = 2 +/- 0.1.
