EFFECT OF 3-HYDROXYPYRIDINE AND SUCCINIC ACID DERIVATIVES ON BACTERIAL GROWTH IN DISCONTINUOUS CULTURES.

We have studied the effect of domestic derivatives of 3-hydroxypyridine and succinic acid (emoxipine, reamberin, and mexidol) on proliferation of E. coli, S. aureus and E. faecalis in discontinuous cultures (DCs) over a period of 1 or 24 h. It is established that the drugs studied exhibit a biphase effect on the number of bacterial colony-forming units (CFUs) in DCs of E. coli and E.faecalis. This effect is manifested by the initial 1.3 - 3.5-fold decrease in the number of CFUs (after 1-h incubation with drugs) with subsequent return to the control level or 1.8 - 8.8-fold increase over the control level (after 24-h incubation with drugs). In DCs of S. aureus that were incubated with the drugs, we observed 1.1 - 1.95-fold increase in CFU number after I-h incubation and 1.8 - 2.4-fold increase after 24-h incubation. On the whole, the results of our studies demonstrated a positive effect of 3-hydroxypyridine and succinic acid derivatives on bacterial growth in DC. These drugs stimulated increase in CFU number in DCs of test bacterial strains even at concentrations as low as 10⁻9 -10⁻6. The well-known efficiency of 3-hydroxypyridine and succinic acid derivatives in treatment of infectious pathology appears independent of their direct effect on bacterial growth.

Effects of 3-hydroxypyridine derivatives and succinic acid on endometrial leukocyte infiltration, cytokinemia and related affective symptoms during exacerbation of the chronic inflammation of the uterus and adnexa.

We studied the effects of original Russian derivatives of 3-hydroxypyridine and succinic acid (emoxipine, reamberin and mexidol) on the time course of endometrial leukocyte infiltration, blood levels of inflammatory cytokines (IL-1ß and TNF-α), and indicators of affective status in women with exacerbation of the chronic inflammation of the uterus and adnexa. It was found that emoxipine, reamberin, and mexidol included in the complex therapy corrects anxiety and depressive disorders depending on the degree of reduction of endometrial leukocyte infiltration and blood levels of inflammatory cytokines. Derivatives of 3-hydroxypyridine (emoxipine and mexidol) exhibited efficiency, which were superior to derivative of succinic acid (reamberin) in the degree of reduction in endometrial leukocyte and neutrophyl infiltration and in severity of anxiety and depressive disorders. Mexidol, a 3-hydroxypyridine and succinic acid derivative, was most effective and surpassed emoxipine by the degree of reduction of inflammatory cytokine level in the blood and the severity of affective anxiety symptoms in women with exacerbation of the chronic inflammation of the uterus and adnexa.

[Effect of 3-oxypyridine and succinic acid derivatives on endometrial leucocyte infiltration and lipid peroxidation in recrudescence of inflammatory diseases of the uterus and its appendages].

The effect of domestic 3-oxypyridine and succinic acid derivatives (emoxipin, reamberin and mexidol) included in the complex treatment of recrudescence of inflammatory diseases of the uterus and its appendages, as manifested in endometrial leucocyte infiltration (ELI) compared to lipid peroxidation products concentration in blood serum, was assessed in a short-term, prospective placebo-controlled single-blind randomized trial. It has been found that two-week infusions of emoxipin (single dose, 150 mg), reamberin (single dose, 400 ml), and mexidol (single dose, 300 mg) favor a decrease in ELI and influence ambiguously on lipid peroxidation - antioxidant (LPA) system condition in blood serum of patients. It has been found that 3-oxypyridine derivative (emoxipin) decreases the intensity of ELI, but does not affect LPA system condition. Succinic acid derivative (reamberin) is inferior to emoxipin in the degree of decreasing ELI, but reduces the concentration of the antioxidant protein ceruloplasmin. Mexidol, being 3-oxipyridine and succinic acid derivative simultaneously, exceeds reamberin in decreasing ELI, increases concentration of blood antioxidant components (alpha-tocopherol and ceruloplasmin), and decreases the level of primary isopropanol-soluble products of lipid peroxidation.

[Effect of 3-oxypyridine and succinic acid derivatives on clinical manifestations of inflammatory diseases of the uterus and its appendages].

Two-week-long, prospective, placebo-controlled, single-blind randomized study of the effects of intravenous emoxipin (150 mg daily), reamberin (400 ml daily) and mexidol (300 mg daily) infusion on the dynamics of clinical symptoms of non-complicated recrudescence of inflammatory diseases of the uterus and its appendages (IDUA) in comparison to the standard therapy was performed. It was established that emoxipin, reamberin and mexidol administration appreciably improved the clinical state dynamics of women with recrudescence of IDUA. The effect was associated with more pronounced decrease in severity of genital and abdominal symptoms versus standard therapy results.

[Effect of 3-oxypyridine and succinic acid derivatives on affective status in recrudescence of inflammatory diseases of uterus and its appendages].

Short-term, prospective placebo-controlled simple blind randomized study of the effects of 3-oxypyridine and succinic acid derivatives (emoxipin, reamberin, mexidol) on the affective status of females with recrudescence of the inflammatory diseases of uterus and its appendages (IDUA) in comparison to changes of systemic inflammatory response (SIR) markers level in the blood has been conducted. It is established that the inclusion of emoxipin, reamberin and mexidol in complex treatment of IDUA recrudescence reduce depression, anxiety and SIR laboratory signs. Mexidol being both 3-oxypyridine and succinic acid derivative showed the best influence on the dynamics of affective disorders and SIR changes.