Physiological selectivity and tolerance of social wasps exposed to Malathion.

Insect pest infestations are a worldwide problem as a result of human actions. Among them, Aedes aegypti stands out as vector of several diseases. Like other pests, A. aegypti has become resistant to the insecticide used for its control, thus harming the local fauna of non-target insects. Therefore, the aim of this study was to study the physiological selectivity of social wasps exposed to Malathion, as well as to investigate possible resistance to this insecticide. Adults of the swarming species Polybia platycephala and Protopolybia sedula and the independent species Polistes versicolor were exposed to four Malathion concentrations. Overall, Malathion was more selective to P. versicolor and more toxic to P. platycephala, and despite the decrease in the concentration of the product recommended by the manufacturer, which in theory would be sublethal doses, social wasps still exhibited high mortality rates. In comparing the mortalities of species collected in an area where there is no application of the insecticide with an area where there is regular application, the data showed an indication that populations of non-target insects are also becoming resistant to insecticides, especially in urban environments.

Modeling the impact of genetically modified male mosquitoes in the spatial population dynamics of Aedes aegypti.

The mosquito Aedes aegypti is the primary vector of diseases such as dengue, Zika, chikungunya, and yellow fever. Improving control techniques requires a better understanding of the mosquito's life cycle, including spatial population dynamics in endemic regions. One of the most promising techniques consists of introducing genetically modified male mosquitoes. Several models proposed to describe this technique present mathematical issues or rely on numerous parameters, making their application challenging to real-world situations. We propose a model describing the spatial population dynamics of the Aedes aegypti in the presence of genetically modified males. This model presents some mathematical improvements compared to the literature allowing deeper mathematical analysis. Moreover, this model relies on few parameters, which we show how to obtain or estimate from the literature. Through numerical simulations, we investigate the impacts of environmental heterogeneity, the periodicity of genetically modified male releases, and released genetically modified males quantity on the population dynamics of Aedes aegypti. The main results point to that the successful application of this vector control technique relies on releasing more than a critical amount of modified males with a frequency exceeding a specific critical value.

Camouflaged Nests of Mischocyttarus mirificus (Hymenoptera, Vespidae).

Social wasps present various architectural patterns for their nests, which may differ in shape, size, color, and material used in construction. The distribution pattern of comb cells presented by Mischocyttarus mirificus (Zikán, 1935) is vertical with a single cell of width, resulting in a filiform shape that camouflages in the middle of the substrate. There are few studies regarding this architectural pattern for social wasps and their role in camouflage, and this study aims to detail the nesting habits of this species. In total, 40 colonies were analyzed in their natural habitat and six were dissected in the laboratory. Aspects about the construction of the nests and nesting environment have been described. A test was carried out with nest photographs, to quantify their camouflage percentage within the nesting substrate. The colonies were found mainly in riparian forests where there were elements in the environment that favored their camouflage. According to the nest photograph analysis, their camouflage levels can vary according to the position from which the photograph is taken. It is evident that M. mirificus founders select sites where they can camouflage their colonies, and this gives them advantages in exploring a new environment.

Reflections on Potential Risk Factors of Callitrichidae Run Over in an Urban Area: A Case Report of Marmoset Deaths.

Interest in the well being of wild animals in their natural habitats is still lower than that of domesticated animals and captive wild animals. Urban development is one of the events that has the greatest impact on fauna, as it affects the survival and well being of wild species in many ways. This study aimed to record death by being run over of two Callithrix penicillata (black-tufted) marmosets in a fragmented environment by urbanization and to discuss how anthropic intervention modifies species behavior and influences their well being, and to suggest measures which can reduce the occurrence of incidents, thereby contributing to maintaining the fauna and their well being.

Hazardous elements in the soil of urban cemeteries; constructive solutions aimed at sustainability.

Urban cemeteries on a global scale raise concerns due to their potential to concentrate differing levels of hazardous pollutants in their native soils due to the unnatural concentration of burials in a limited space. It is paramount for sustainability that designers of future cemeteries take this into account in order to minimize the deposition and movement of these contaminants within the soil profile. The objective of this manuscript is to identify the levels of certain hazardous element contamination, specifically heavy metals, in the soil of horizontal urban cemeteries that do not utilize herbicides for weed control. In this, solutions were sought for the construction of future urban cemeteries capable of mitigating further contamination of the environment by the increase in interments. The soils of three urban cemeteries (A, B and C) in the Brazilian city of Carazinho, in Rio Grande do Sul State, were sampled with 5 monitoring points in the internal area and 5 points in the external area of the cemeteries. At each point, 3 replications were performed at two depths (0-20 and 20-40 cm), totaling 180 samples in all, to determine the concentration of the following metals: copper (Cu), zinc (Zn), iron (Fe), manganese (Mn), lead (Pb), and chromium (Cr) (g kg-1). In addition, online interviews with 15 architects who design cemeteries were conducted. Architectural design solutions to mitigate environmental contamination were modeled utilizing the Building Information Modeling system (BIM). The results showed an excess of Cu in the soil of cemeteries A, B and C, surpassing the standards allowed by Brazilian federal regulations. A total of 80% of the interviewed architects expressed their preference for the vertical cemetery, with gas and effluent treatment systems to mitigate environmental impacts.

Pest Control Potential of Social Wasps in Small Farms and Urban Gardens.

In environments undergoing constant transformation due to human action, such as deforestation and urbanization, the emergence of pests has become a challenge for agriculture and human welfare. In Brazil, over a thousand tonnes of pesticides are used annually, causing serious environmental damage such as the decline of insect populations. It is necessary to search for control alternatives in order to reduce the environmental impact caused by insecticides. This review aims to describe the use of social wasps as agents of biological control, focusing on the perspectives of their use in small farms and urban gardens, and to discuss the benefits of using this method. Studies have shown that 90-95% of the prey captured by wasps in small crops is made of leaf-eating caterpillars. In urban gardens, wasps diversify their prey, among which potential disease vectors, such as dipterans, stand out. We outline techniques for managing social wasp colonies in small farm and urban garden settings, including the use of artificial shelters. Among the advantages of using wasps as control agents, we highlight the practicality of the method, the low operational cost, the absence of prey resistance and the decrease of the use of insecticides.

Record and foraging behavior of ants (Hymenoptera, Formicidae) in vertebrate carcasses / Registro de forrageio de formigas (Hymenoptera, Formicidae) em carcaças de vertebrados

Knowing the importance of participation by insects at cadaverous decomposition processes, and the limited use of the family Formicidae in criminal investigations, this study aims to record the foraging activity of four genera of ants in carcasses of birds and mammals. Observations occurred accidentally in two locations in the State of Minas Gerais, Brazil. In total, seven species of ants foraging in eight vertebrate carcasses were recorded. In addition, the study reported for the first time the presence of Wasmannia in carcasses in Brazil.

Sabendo da importância da participação dos insetos em processos de decomposição cadavérica e do uso limitado da família Formicidae em investigações criminais, o objetivo do presente estudo foi registrar a atividade de forrageio de quatro gêneros de formigas em carcaças de aves e mamíferos. As observações ocorreram de forma acidental em duas localidades do Estado de Minas Gerais, Brasil. No total, foram registradas sete espécies de formigas forrageando em oito carcaças de vertebrados. O trabalho registrou ainda pela primeira vez a presença do gênero Wasmannia em carcaças no Brasil.

Flower-Visiting Insects and Phenology of Lippia alba (Lamiales: Verbenaceae): Floral Color Changes and Environmental Conditions as Cues for Pollinators.

Lippia alba (Mill.) N.E. Br. ex Britton & P. Wilson (Verbenaceae) is a herbal aromatic shrub with medicinal properties. Natural populations of this species are allogamous and self-incompatible. Therefore, this plant species relies on pollinators to outcross and maintain the genetic variability. Here, we investigated the floral phenology, pollen morphology, the floral visitor entomofauna, and the influence of climatic factors on the frequency of visits in L. alba flowers. The study was conducted at Federal University of Juiz de Fora Plant Experimental Area, southeast Brazil. The flowering of L. alba occurred throughout the whole year. The anthesis is diurnal and lasts 5 d. The nectar guide was visible from the onset of flowering until the third day. Pollen grains were classified as isopolar, oblate spheroidal, three-colporate (rare, four-colporate), surface tectate-perforate, and endoaperture lalongate, with median constriction. Insects from the order Hymenoptera were the most frequent visitors observed, followed by Lepidoptera, Thysanoptera, Diptera, and Hemiptera-Heteroptera. The preference of insects for flowers with nectar guides shows that the color is likely to be an important visual trait to increase the frequency of visits. Moreover, the climate variables temperature, relative humidity, wind speed, and light were important to define the composition of the most frequent floral visitors.

Marine debris ingestion by sea turtles (Testudines) on the Brazilian coast: an underestimated threat?

Assessment of marine debris ingestion by sea turtles is important, especially to ensure their survival. From January to December 2011, 23 specimens of five species of sea turtles were found dead or dying after being rehabilitated, along the coast of the municipality of Rio de Janeiro, Brazil. To detect the presence of marine debris in the digestive tract of these turtles, we conducted a postmortem examination from the esophagus until the distal portion of the large intestine for each specimen. Of the total number of turtles, 39% had ingested marine debris such as soft plastic, hard plastic, metal, polyethylene terephthalate (PET) bottle caps, human hair, tampons, and latex condoms. Five of the seven sea turtles species are found along the Brazilian coast, where they feed and breed. A large number of animals are exposed to various kinds of threats, including debris ingestion.

Myrmecofauna of urban gardens in southeast region of Brazil / Mirmecofauna de jardins urbanos na região sudeste do Brasil

In an urban environment, ants can find shelter for the construction of their nests and resources available for survival. Myrmecofauna surveys have been conducted in forest fragments and residential areas, but there are few studies on the occurrence of these insects in urban gardens. For this reason, the aim of this study is to know the composition of ant communities in urban gardens in the city of Juiz de Fora, southeast region of Brazil. Samples were taken from six gardens between November 2005 and June 2006. We used a consortium of three methodologies: active searching, baits and pitfall traps. We collected specimens of 26 genera and seven subfamilies. The subfamily Myrmicinae was the most diverse, and the genus Wasmannia, was considered the most constant, followed by Camponotus and Linepithema. The efficiency index of the methodologies demonstrated that active searching was the most efficient (96.15%) methodology. This method is efficient and sufficient for sampling ants in urban gardens, since 25 of the 26 genera sampled, presenting seven exclusive genera. The green area was not positively correlated with the diversity of ant genera in the urban gardens, demonstrating that other variables may be involved.

Em ambiente urbano, as formigas podem encontrar abrigo para construção de seus ninhos e recursos disponíveis para sobrevivência. Levantamentos da mirmecofauna já foram realizados em fragmentos de mata e áreas domiciliares, porém existem poucos estudos quanto à ocorrência destes insetos em jardins urbanos. Por esta razão, objetivou-se realizar o levantamento da mirmecofauna presente em jardins urbanos do município de Juiz de Fora, sudeste do Brasil. Foram realizadas coletas em seis jardins entre novembro de 2005 e junho de 2006. Foi utilizado o consórcio de três metodologias: busca ativa, isca atrativa e pitfall. Foram coletados exemplares de 26 gêneros e sete subfamílias. A subfamília Myrmicinae foi a mais diversa e o gênero Wasmannia considerado o mais constante, seguido por Camponotus e Linepithema. O índice de eficiência das metodologias demonstrou que a busca ativa foi a metodologia mais eficiente de coleta (96,15%). Este método é eficiente e suficiente para a coleta de formigas em jardins urbanos, uma vez que 25 dos 26 gêneros foram amostrados, com presença de sete gêneros exclusivos. A área verde não esteve correlacionada positivamente com a diversidade de gêneros de formigas nas praças, demonstrando que outras variáveis devem estar envolvidas.

Sexual behavior of the digger wasp Sphex ingens Smith (Hymenoptera, Sphecidae)

A species' mating system depends on its spatial distribution and temporal availability of mating opportunities, as well as on the resources that create these opportunities. In addition, for many species, courtship is driven by specific behaviors that precede and follow copulation. Although Sphex ingens is a taxonomically well known species of digger wasp, its ecology and behavior remain poorly known. Hence, we analyzed patterns and trends of sexual behavior, in order to understand whether courtship can persist in a polygamous mating system. We monitored by video wasp populations in Ilha Grande, southeastern Brazil. Based on the observed behaviors, we calculated stochastic probabilities with a Markov chain to infer on behavioral trends. We recorded four behavioral phases based on 19,196 behavioral acts observed in 224 copulation attempts. There were no significant differences in common behavioral acts between males and females. The copulation patterns, conflicts, and trends observed in S. ingens clearly show the influence of sexual selection in its promiscuous mating system.

The ant fauna of hospitals: advancements in public health and research priorities in Brazil

Ants inhabit several types of natural and urban habitats, where they successfully nest. In urban environments, the hospitals should be considered priority for studies, as ants pose risks to human health due to their pathogen carrying potential. We aimed at surveying the literature about studies on ants in hospital settings in Brazil in the past 20 years. We found 40 papers in 22 journals, the first one published in 1993. Among them, 26 papers assessed pathogenic microorganisms on ants. We recorded 59 ant species, being Tapinoma melanocephalum the most common. The Minas Gerais and São Paulo states had the largest number of published papers. Mato Grosso do Sul and Rio Grande do Sul showed the highest number of species. Exotic ant species were recorded in all states, except Goiás. Considering the potential to carry microorganisms and the importance of thorough studies on the ecology of ant species, our results can support and guide further research in Brazil.

Nesting substrata, colony success and productivity of the wasp Mischocyttarus cassununga

Nesting substrata, colony success and productivity of the wasp Mischocyttarus cassununga. Colonies of the wasp Mischocyttarus cassununga (von Ihering, 1903) are easily found in urban areas. However, in spite of the massive presence of this species in cities, little is known about its nesting habits, colony success and productivity. The present study aimed at answering the following questions: What are the substrates used for nesting by M. cassununga? What is the main foundation strategy adopted by M. cassununga in urban areas: a solitary female or associative foundation? Is there a relationship between foundation strategies and colony success? Is the total number of cells per nest related to the number of adults produced? The study was conducted in Juiz de Fora, southeastern Brazil, from December 2006 to November 2007. Nesting in man-made substrata seems to be a common strategy in M. cassununga (90.9%), with preference for nest building with a horizontal comb facing north. The colonies were established mainly by groups of foundresses (67.6%), with a success of 84%. The number of brood cells produced per nest was 71.74 ± 45.25 (18-203), and it was positively correlated with the number of adults produced. Hence, we can say that the nests founded by M. cassununga are located mainly in man-made substrata and mostly founded by a group of females. The cell reuse behavior increases the number of adults produced, as it optimizes foraging. These characteristics together with its behavior and nesting habits promote the success of this species in thriving in urban environments.

Seasonal richness and composition of social wasps (Hymenoptera, vespidae) in areas of cerrado biome in Barroso, Minas Gerais, Brazil / Riqueza sazonal e composição de vespas sociais (Hymenoptera, vespidae) em áreas de cerrado em Barroso, Minas Gerais, Brasil

The study evaluated the seasonal richness and composition of social wasps in Cerrado transition areas and Atlantic Forest in the municipal district of Barroso (Cerrado Grassland, Semidecidual Forest and Anthropic Areas). Thirty-eight species of social wasps were recorded, distributed in 10 genera and 127 colonies. The phytophysiognomy of Cerrado Grassland had the highest number of species (35) and colonies (76), followed by Semidecidual Forest (26 species and 37 colonies) and Anthropic Areas (nine species and 14 colonies). Agelaia vicina (Saussure, 1854) showed the highest constancy, present in 57.7% of collections. The number of social wasp species and colonies was positively correlated with temperature and precipitation. The hot and humid season is the most favorable period for social wasp population growth.

A riqueza sazonal e composição de vespas sociais em áreas de transição Cerrado e Mata Atlântica no município de Barroso foram avaliadas (campo cerrado, mata semidecidual e áreas antrópicas). Foram registradas 38 espécies de vespas sociais, distribuídas em 10 gêneros, e 127 colônias. A fitofisionomia de campo cerrado teve o maior número de espécies (35) e de colônias (76), seguido da mata semidecidual (26 espécies e 37 colônias) e áreas antrópicas (nove espécies e 14 colônias). Agelaia vicina (Saussure, 1854) apresentou maior constância, presente em 57,7% das coletas. O número de espécies e colônias de vespas sociais correlacionou positivamente com a temperatura e a precipitação. A estação quente e úmida é o período mais favorável para o crescimento populacional de vespas sociais.

Nidification of Polybia rejecta (Hymenoptera: Vespidae) associated to Azteca chartifex (Hymenoptera: Formicidae) in a fragment of Atlantic Forest, in the state of Minas Gerais, southeastern Brazil / Nidificação de Polybia rejecta (Hymenoptera: Vespidae) associada com Azteca chartifex (Hymenoptera: Formicidae) em um fragmento de Mata Atlântica, no estado Minas Gerais, Sudoeste do Brasil

Records to nesting between associated social wasp Polybia rejecta (Fabricius, 1798) and the ant Azteca chartifex Forel, 1896 in the Parque Estadual do Rio Doce, Minas Gerais, are reported. This association is reported for the first time in this biome. Twelve colonies of P. rejecta founded close to nests of A. chartifex were observed. The wasp colonies were founded at a distance of about 10-20 cm from the ant nest and they did not overlap. The nests of wasps were smaller compared to ant, but the coloring was similar protective casing, making it difficult to differentiate between the wasp nest and the ant.

Registra-se a nidificação associada entre a vespa social Polybia rejecta (Fabricius, 1798) e a formiga Azteca chartifex Forel, 1896 no Parque Estadual do Rio Doce, Minas Gerais. Esta associação é observada neste bioma pela primeira vez. Observaram-se doze colônias de P. rejecta fundadas ao lado de ninhos da A. chartifex . Cada colônia da vespa social foi fundada a uma distância em torno de 10 a 20 cm do ninho da formiga, não ocorrendo sobreposição das colônias. Os ninhos das vespas eram menores em relação a formiga, porém a coloração do invólucro protetor era semelhante, o que dificultava a diferenciação entre os ninho da vespa e da formiga.

Padrões comportamentais de visitantes na área do Parque do Museu Mariano Procópio, Juiz de Fora, MG / Behavior of visitors in the Park of the Mariano Procópio Museum, Juiz de Fora, MG

Este estudo teve como objetivo identificar o comportamento dos visitantes frente aos animais mantidos na área do Parque do Museu Mariano Procópio, Juiz de Fora, MG. O trabalho foi realizado nos meses de maio e junho de 2005. As observações foram feitas através da amostragem scan (n=1085) em três pontos, e os comportamentos classificados em cinco categorias: admirar, observar, alimentação, desinteresse e interação. Classificaram-se os visitantes em: indivíduo criança; indivíduo não criança; grupo composto por criança, grupo sem criança e grupo misto. O comportamento mais frequente foi a observação (n=301, 28 por cento), seguido de admiração (n=259; 24 por cento), desinteresse (n=248, 23 por cento), interação (n=163, 14 por cento) e alimentação (n=114, 11 por cento). Crianças se interessam mais por observar e interagir com os animais do que indivíduos não crianças. Através do entendimento do comportamento de visitantes de áreas verdes é possível a elaboração de estratégias educativas.(AU)

We recorded the behavior of visitors in relation to the captive animals in the park of the Mariano Procópio Museum, Juiz de Fora, MG. The work was executed during the months of May and June, 2005. Individuals were scanned (n=1085), in three points, and the behaviors classified in five categories: to admire, to observe, to feed, blandness and interaction. The visitors were classified as: child, not child, group of children, group without children and mixed group. The most frequent behavior was observation (n=301, 28 percent), followed by admiration (n=259; 24 percent), blandness (n=248, 23 percent), interaction (n=163, 14 percent) and feeding (n=114, 11 percent). Children observe and interact more with animals than do other groups. Through the understanding of the behavior of visitors in environmental areas it is possible to make the elaboration of the educational strategy.(AU)

Padrões comportamentais de visitantes na área do Parque do Museu Mariano Procópio, Juiz de Fora, MG / Behavior of visitors in the Park of the Mariano Procópio Museum, Juiz de Fora, MG

Este estudo teve como objetivo identificar o comportamento dos visitantes frente aos animais mantidos na área do Parque do Museu Mariano Procópio, Juiz de Fora, MG. O trabalho foi realizado nos meses de maio e junho de 2005. As observações foram feitas através da amostragem scan (n=1085) em três pontos, e os comportamentos classificados em cinco categorias: admirar, observar, alimentação, desinteresse e interação. Classificaram-se os visitantes em: indivíduo criança; indivíduo não criança; grupo composto por criança, grupo sem criança e grupo misto. O comportamento mais frequente foi a observação (n=301, 28 por cento), seguido de admiração (n=259; 24 por cento), desinteresse (n=248, 23 por cento), interação (n=163, 14 por cento) e alimentação (n=114, 11 por cento). Crianças se interessam mais por observar e interagir com os animais do que indivíduos não crianças. Através do entendimento do comportamento de visitantes de áreas verdes é possível a elaboração de estratégias educativas.

We recorded the behavior of visitors in relation to the captive animals in the park of the Mariano Procópio Museum, Juiz de Fora, MG. The work was executed during the months of May and June, 2005. Individuals were scanned (n=1085), in three points, and the behaviors classified in five categories: to admire, to observe, to feed, blandness and interaction. The visitors were classified as: child, not child, group of children, group without children and mixed group. The most frequent behavior was observation (n=301, 28 percent), followed by admiration (n=259; 24 percent), blandness (n=248, 23 percent), interaction (n=163, 14 percent) and feeding (n=114, 11 percent). Children observe and interact more with animals than do other groups. Through the understanding of the behavior of visitors in environmental areas it is possible to make the elaboration of the educational strategy.

Foundation pattern, productivity and colony success of the paper wasp, Polistes versicolor.

Polistes versicolor (Olivier) (Hymenoptera: Vespidae) colonies are easily found in anthropic environments; however there is little information available on biological, ecological and behavioral interactions of this species under these environmental conditions. The objective of this work was to characterize the foundation pattern, the productivity, and the success of colonies of P. versicolor in anthropic environments. From August 2003 to December 2004, several colonies were studied in the municipal district of Juiz de Fora, Southeastern Brazil. It was possible to determine that before the beginning of nest construction the foundress accomplishes recognition flights in the selected area, and later begins the construction of the peduncle and the first cell. As soon as new cells are built, the hexagonal outlines appear and the peduncle is reinforced. Foundation of nests on gypsum plaster was significantly larger (p < 0.0001; chi2 test) in relation to the other types of substrate, revealing the synantropism of the species. On average, the P. versicolor nest presents 244.2 ± 89.5 (100-493) cells and a medium production of 171.67 ± 109.94 (37-660) adults. Cells that produced six individuals were verified. Usually, new colonies were founded by an association of females, responsible for the success of 51.5%. Although these results enlarge knowledge on the foundation pattern of P. versicolor in anthropic environments, other aspects of the foundation process require further investigation.

An alternative technique for individual marking of orb-web spiders / Uma técnica alternativa para a marcação individual de aranhas orbitelas

A technique is proposed for individually marking orb-web spiders aiming at facilitating the identification of the individuals on their webs. To check the efficiency of the method, 17 females and 30 males of Nephila clavipes (Linnaeus 1767) were marked on the dorsal part of the opisthosoma using enamel applied through a disposable syringe. Success was obtained in marking 47 individuals; no death, dispersal or agonistic behavior was observed after the use of the method. This marking did not dissolve in contact with water, resisted the expansion of the opisthosoma and was lost only in the ecdysis. This method also was efficient for males that, being smaller than the females, demand a more careful methodology(AU)

Uma técnica alternativa para a marcação individual de aranhas orbitelas. Propõe-se uma técnica de marcação individual de aranhas orbitelas visando facilitar a identificação dos indivíduos em suas teias. Para verificar a eficiência do método, 17 fêmeas e 30 machos de Nephila clavipes (Linnaeus, 1767) foram marcados na parte dorsal do abdômen utilizando-se esmalte, aplicado por meio de seringa descartável. Obteve-se sucesso na marcação dos 47 indivíduos, não sendo observada morte, dispersão ou comportamentos agonísticos após o uso do método. Esta marcação não dissolveu em contato com a água, resistiu ao aumento do opistossoma e foi perdida somente na ecdise. Esse método também foi eficiente para os machos que, por serem menores que as fêmeas, exigem uma metodologia mais cuidadosa(AU)

An alternative technique for individual marking of orb-web spiders / Uma técnica alternativa para a marcação individual de aranhas orbitelas

A technique is proposed for individually marking orb-web spiders aiming at facilitating the identification of the individuals on their webs. To check the efficiency of the method, 17 females and 30 males of Nephila clavipes (Linnaeus 1767) were marked on the dorsal part of the opisthosoma using enamel applied through a disposable syringe. Success was obtained in marking 47 individuals; no death, dispersal or agonistic behavior was observed after the use of the method. This marking did not dissolve in contact with water, resisted the expansion of the opisthosoma and was lost only in the ecdysis. This method also was efficient for males that, being smaller than the females, demand a more careful methodology

Propõe-se uma técnica de marcação individual de aranhas orbitelas visando facilitar a identificação dos indivíduos em suas teias. Para verificar a eficiência do método, 17 fêmeas e 30 machos de Nephila clavipes (Linnaeus, 1767) foram marcados na parte dorsal do abdômen utilizando-se esmalte, aplicado por meio de seringa descartável. Obteve-se sucesso na marcação dos 47 indivíduos, não sendo observada morte, dispersão ou comportamentos agonísticos após o uso do método. Esta marcação não dissolveu em contato com a água, resistiu ao aumento do opistossoma e foi perdida somente na ecdise. Esse método também foi eficiente para os machos que, por serem menores que as fêmeas, exigem uma metodologia mais cuidadosa.