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ABSTRACT

Aim: To research the synergistic activity of G2-S16 dendrimer and dapivirine (DPV) antiretroviral as microbicide candidate to prevent HIV-1 infection. Materials & methods: We assess the toxicity of DPV on cell lines by MTT assay, the anti-HIV-1 activity of G2-S16 and DPV alone or combined at several fixed ratios. Finally, their ability to inhibit the bacterial growth in vitro was assayed. The analysis of combinatorial effects and the effective concentrations were performed with CalcuSyn software. Conclusion: Our results represent the first proof-of-concept study of G2-S16/DPV combination to develop a safe microbicide.

Subject(s)

Anti-HIV Agents/pharmacology , Dendrimers/pharmacology , Epithelial Cells/drug effects , HIV-1/drug effects , Pyrimidines/pharmacology , Silanes/pharmacology , Animals , Anti-HIV Agents/administration & dosage , Anti-HIV Agents/toxicity , Bacteria, Anaerobic/drug effects , Cell Line, Tumor , Cell Survival/drug effects , Chlorocebus aethiops , Dendrimers/administration & dosage , Dendrimers/toxicity , Dose-Response Relationship, Drug , Drug Synergism , Epithelial Cells/virology , HIV Infections/prevention & control , Humans , Microbial Sensitivity Tests , Polyelectrolytes , Polymers , Pyrimidines/administration & dosage , Pyrimidines/toxicity , Silanes/administration & dosage , Silanes/toxicity , Vero Cells

ABSTRACT

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