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4.
Biull Eksp Biol Med ; 99(4): 460-2, 1985 Apr.
Article in Russian | MEDLINE | ID: mdl-3921083

ABSTRACT

UV irradiation (254 nm) in doses increasing erythrocyte (Er) hemolysis by 5 to 32% was found to stimulate the agglutination activity of ABO and Rhesus (Rh) system antigens. The stimulation effect was the higher the lower the antigen activity before irradiation. In the Rh-negative (Rh-)-Er, irradiation induced the Rh0(D)-like antigen specific activity suggesting that this antigen may be present in the Rh--Er membrane. Expression of Rh0(D)-antigen in Rh--Er, stimulation of its activity in Rh-positive cells, and activation of ABO system antigens may result from photo-chemical destruction of the outer membranous layer of the ER.


Subject(s)
ABO Blood-Group System/immunology , Antigens/radiation effects , Blood Donors , Erythrocytes/immunology , Rh-Hr Blood-Group System/immunology , Ultraviolet Rays , Agglutination Tests , Antigens/analysis , Dose-Response Relationship, Radiation , Erythrocytes/radiation effects , Humans
6.
Tsitologiia ; 25(12): 1378-86, 1983 Dec.
Article in Russian | MEDLINE | ID: mdl-6422597

ABSTRACT

UV irradiation (254 nm) in doses increasing erythrocyte haemolysis by 5, 10, 18 and 28 per cent was found to stimulate, by 2--16 times, the agglutination activity of ABO and Rh system antigens. The stimulation effect was the higher the lower the antigen activity before irradiation. In the Rh-negative (Rh-) erythrocytes, irradiation induced manifestation of the Rh0(D)-antigen specific activity suggesting that this antigen may be present in the Rh- erythrocyte membrane. The expression of Rh0(D)-antigen in Rh- erythrocytes, the stimulation of its activity in Rh-positive cells, and the activation of ABO system antigens may result from a photochemical destruction of the outer perimembraneous layer and release some of its components which stain in situ with alcian blue to be presumably glycoproteins. This effect is necessary to keep in mind when UV-irradiated blood transfusion is performed in therapeutic aims Rh- patients.


Subject(s)
ABO Blood-Group System/immunology , Antigens, Surface/radiation effects , Erythrocyte Membrane/radiation effects , Rh-Hr Blood-Group System/immunology , Ultraviolet Rays , Antigens, Surface/analysis , Dose-Response Relationship, Radiation , Erythrocyte Membrane/immunology , Hemagglutination Tests , Hemolysis/radiation effects , Humans , Surface Properties , Time Factors
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