ABSTRACT
Effective methods for making semithin sections of large surface area are not currently available. An alternative solution to this problem, without actual histological section preparation, is proposed. This is a modification of a combination of methods for fixing tissues and embedding them in epoxide resin using known technical methods. This method for making preparations for microscopic examination of biological objects with large surface areas has additional valuable potential, as it allows investigations of a variety of complexes of soft and hard (cartilaginous and bony) tissues without prior decalcination.
Subject(s)
Histocytological Preparation Techniques , Microscopy, Electron, Transmission/methods , Epoxy Resins , Microscopy/methods , Microscopy, Electron, Scanning/methods , Tissue Embedding/methodsABSTRACT
The effective methods for producing large-area semithin sections are presently still unavailable. The method is suggested to alternatively solve this problem without the very procedure of histological sectioning. It includes a modified combination of the methods of tissue fixation and embedding in epoxy resin with the known technique of preparing the ground sections. This method of slide preparation for the microscopical study of large area biological objects gives an additional valuable opportunity, since it allows to study heterogeneous complexes consisting of soft and hard (cartilaginous and bone) tissues without their prior decalcination.
