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1.
Genetika ; 52(2): 189-97, 2016 Feb.
Article in Russian | MEDLINE | ID: mdl-27215033

ABSTRACT

For the first time, genetic analysis of the cytochrome b gene fragment haplotypes encoding the identical and the most common cytochrome b polypeptide (F1) in M. rutilus from Eastern and Beringian mateml lineages was carried out. The F1 frequencies for the vole populations from Northern Priokhotye and the Kolyma basin were calculated. Considerable polymorphism of the cytochrome b F1 haplotypes within two major phylo- groups of red-backed vole was supported by high molecular diversity indices for these clades. The proportion of genetic variation between the maternal lineages of F1 red-backed vole individuals (60.71%) was considerably higher than inter-(24.44%) and intrapopulation (14.85%) components. The data obtained make it possible to advance a hypothesis on the convergence of the cytochrome b polypeptide structure upon sequence divergence of the corresponding gene.


Subject(s)
Arvicolinae/genetics , Cytochromes b/genetics , Genetic Variation , Phylogeography , Animals , DNA, Mitochondrial/genetics , Genetics, Population , Haplotypes , Polymorphism, Genetic
2.
Izv Akad Nauk Ser Biol ; (4): 334-40, 2014.
Article in Russian | MEDLINE | ID: mdl-25735175

ABSTRACT

Data regarding the structure and variation of the nucleotide sequence of the cytochrome b gene of mitochondrial DNA of the Siberian Sucker from the Kolyma River were obtained. Analysis of the median network revealed that evolutionary lines diverged from a common ancestor. Penetration of the sucker into Asia from Northern America took place between the Early and Middle Pleistocene. Prolonged reproductive isolation of the Siberian and Northern American suckers led to interspecies divergence with the appearance of amino acid substitutions, which, apparently, fixed due to positive selection. The Siberian Sucker appeared to have three modifications of the Cytb protein.


Subject(s)
Cypriniformes/genetics , Cytochromes b/genetics , DNA, Mitochondrial/genetics , Phylogeny , Animals , Base Sequence , Genetic Variation , Siberia
3.
Izv Akad Nauk Ser Biol ; (3): 283-8, 2011.
Article in Russian | MEDLINE | ID: mdl-21793251

ABSTRACT

The intrapopulation variability of a cytochrome b gene fragment and the corresponding amino acid sequence was studied in the red vole Clethrionomys rutilus Pallas, 1779 from the flood-plain of the Kolyma River. Wide polymorphic variability of these properties was observed. Differences in the cytochrome b gene sequence were determined between the red voles of the studied population and the species collected in neighborhoods of Novosibirsk and Omsk. The revealed results point to the urgency of the cytochrome b gene nucleotide sequence and the variants of the respective amino acid sequence as genetic markers of originality of different red vole populations.


Subject(s)
Arvicolinae/genetics , Cytochromes b/genetics , DNA, Mitochondrial/genetics , Polymorphism, Genetic , Amino Acid Sequence , Animals , Base Sequence , Biomarkers , Evolution, Molecular , Mitochondria/genetics , Phylogeny , Russia , Species Specificity
4.
Genetika ; 37(5): 708-11, 2001 May.
Article in Russian | MEDLINE | ID: mdl-11436565

ABSTRACT

A method for detecting activities of mannose- and glucose-6-phosphate isomerases based on enzyme production of the substrates is described. The results obtained for several animal taxa are illustrated by photographs.


Subject(s)
Glucose-6-Phosphate Isomerase/metabolism , Mannose-6-Phosphate Isomerase/metabolism , Animals , Electrophoresis, Polyacrylamide Gel
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