Anticoagulant heparan sulfate proteoglycans expression in the rat ovary peaks in preovulatory granulosa cells.

Ovarian granulosa cells synthesize anticoagulant heparan sulfate proteoglycans (aHSPGs), which bind and activate antithrombin III. To determine if aHSPGs could contribute to the control of proteolytic activities involved in follicular development and ovulation, we studied the pattern of expression of these proteoglycans during the ovarian cycle. aHSPGs were localized on cells and tissues by (125)I-labeled antithrombin III binding followed by microscopic autoradiography. Localization of aHSPGs has shown that cultured granulosa cells, hormonally stimulated by gonadotropins to differentiate in vitro, up-regulate their synthesis and release of aHSPGS: In vivo, during gonadotropin-stimulated cycle, aHSPGs are present on granulosa cells of antral follicles and are strongly labeled in preovulatory follicles. These data demonstrate that aHSPG expression in the ovarian follicle is hormonally induced to culminate in preovulatory follicles. Moreover, we have shown that five heparan sulfate core proteins mRNA (perlecan; syndecan-1, -2, and -4; and glypican-1) are synthesized by granulosa cells, providing attachment for anticoagulant heparan sulfate chains on the cell surface and in the extracellular matrix. These core proteins are constantly expressed during the cycle, indicating that modulations of aHSPG levels observed in the ovary are likely controlled at the level of the biosynthesis of anticoagulant heparan sulfate glycosaminoglycan chains. This expression pattern enables aHSPGs to focus serine protease inhibitors in the developing follicle to control proteolysis and fibrin formation at ovulation.

[Applications of digital radiology to pediatric uroradiology]. / Applicazione della radiologia digitale all'uroradiologia pediatrica.

The authors report their 4 years' experience with a computerized digital radiographic system based on photostimulable phosphor plates which was used mostly in pediatric radiology. Major protection advantages were observed for uroradiology where high kV (90-95 kV) and very low exposure times (0.02-0.010s) were used. With this radiographic technique reduced exposure parallels increased graininess due to quantum mottle: the two variables must therefore be balanced not to decrease diagnostic accuracy. During our 4 years' experience, 368 examinations were performed on pediatric patients: 252 micturition cystourethographs, 81 urograms and 35 retrograde pyelographs. None of these examinations needed to be repeated due to over/under-exposure and image quality was always high, in spite of a light "noise". Digital radiography in pediatric urology allows radiation dose to be reduced and high quality images to be acquired and is therefore likely to take over conventional radiologic techniques.

[Diffuse idiopathic skeletal hyperostosis. Review of diagnostic criteria and analysis of 915 cases]. / L'iperostosi scheletrica idiopatica diffusa. Revisione dei criteri diagnostici dall'analisi di 915 casi.

DISH is a common systemic skeletal disease, probably of dysmetabolic and/or degenerative origin, yet of unknown etiology. It is observed in middle-aged or elderly patients of both sexes, and is characterized by ossification of the anterior longitudinal ligament on the antero-lateral aspect of the spine, and by ossifying enthesopathy, in both the central and the peripheral skeleton. Diagnosis is solely based on radiographic abnormalities, according to the so-called Resnick criteria. In the present study, the spines of 915 patients (414 males, 501 females, mean age: 65 years) were considered, and the peripheral entheses (heel, patella and elbow) of 494 of them (234 males and 260 females). The incidence of DISH was 14.09% (129 cases): 17.6% in males (73 cases) and 11.7% in females (56 cases). DISH strikes in the VI and VII decades of life most. The most affected sites of the spine were: the dorsal portion (100%), especially in the D7-D11 segment (93%); the lumbar spine in L1-L3 (81%), and the cervical spine, in the C5-C7 segment (69%). Peripheral areas of involvement were: pelvis (90%), heel (76%), elbow (46%) and knee (29%). The symptoms of DISH must be promptly detected: the disease is not asymptomatic, but presents with pain and stiffness in the spine, recurrent tendinitis and bursitis, and myelopathy.

[Role of triphasic bone scintigraphy in the diagnosis of osteonecrosis of the femur head]. / Ruolo della scintigrafia ossea trifasica nella diagnosi della osteo-necrosi della testa femorale.

Twelve patients affected by idiopathic osteonecrosis of the femoral head underwent X-ray, triphasic bone scintigraphic (TBS) and CT tests on the femoral head on both sides. TBS revealed arterial hypoperfusion and an area of hypocaptation in the caput femoris in 3 coxofemoral joints which were radiologically "negative" (and of which one was also CT negative); these are scintigraphical signs which are typical of aseptic necrosis. In line with published data, TBS was found to be a more sensitive test in comparison with traditional X-ray techniques and CT, and in the early stages of disease it is more specific in recognising aseptic necrosis of the femoral head. Lastly, it is underlined that magnetic resonance is currently considered to be most sensitive (88%) and most specific (100%) imaging technique in the diagnosis of osteonecrosis of the femoral head.

[Echographic diagnosis of Peyronie's disease]. / Diagnosi ecografica della malattia di Peyronie.

Thirty-six patients with Peyronie's disease were evaluated with diagnostic sonography. Radiographic and/or xeroradiographic examinations were also performed on 23 patients. Sonography could demonstrate the presence of the plaques characteristic of the disease in the majority of cases (94.5%), whereas only calcified lesions were depicted by conventional X-rays. Sonography is therefore recommended for its well-known advantages over other techniques, not only as the diagnostic methodology of choice, but also in the follow-up of the disease.