Forensic geoscience non-invasive detection and characterisation of underground clandestine complexes, bunkers, tunnels and firing ranges.

Recent events in conflict zones have emphasized that the successful detection and characterisation of buried clandestine complexes, bunkers and tunnels is vitally important for forensic investigators globally, to reduce or solve criminal activities, address national security threats and avoid potential terrorist attacks. However, this can often prove very difficult, particularly in urban areas, with potentially both below-ground non target items and above-ground infrastructures present, that can interfere with detecting target(s). Here we provide selected successful case studies where forensic geoscience techniques were used to detect and characterise buried clandestine complexes, bunkers and tunnels using different geophysical techniques. Generally, desktop studies assessing pre-existing information, including local geology, soils, historical/modern remote sensing, maps and photographs inform appropriate geophysical survey technique(s) selection. Subsequent near-surface geophysical techniques are then employed to produce accurate plans of sub-surface targets, with numerical modelling and correction for the interfering effects of above ground infrastructure, enabling the calibration of geophysical datasets to provide confidence in their respective interpretations. All forensic investigations are, of course, unique to every site, and thus require an individual approach to their respective ground conditions. Investigations should be both phased and iterative, with techniques tailored to local conditions: the selection of geophysical method(s) is crucial to improve successful detection rates of such important buried targets.

The International Olympic Committee framework on fairness, inclusion and nondiscrimination on the basis of gender identity and sex variations does not protect fairness for female athletes.

The International Olympic Committee (IOC) recently published a framework on fairness, inclusion, and nondiscrimination on the basis of gender identity and sex variations. Although we appreciate the IOC's recognition of the role of sports science and medicine in policy development, we disagree with the assertion that the IOC framework is consistent with existing scientific and medical evidence and question its recommendations for implementation. Testosterone exposure during male development results in physical differences between male and female bodies; this process underpins male athletic advantage in muscle mass, strength and power, and endurance and aerobic capacity. The IOC's "no presumption of advantage" principle disregards this reality. Studies show that transgender women (male-born individuals who identify as women) with suppressed testosterone retain muscle mass, strength, and other physical advantages compared to females; male performance advantage cannot be eliminated with testosterone suppression. The IOC's concept of "meaningful competition" is flawed because fairness of category does not hinge on closely matched performances. The female category ensures fair competition for female athletes by excluding male advantages. Case-by-case testing for transgender women may lead to stigmatization and cannot be robustly managed in practice. We argue that eligibility criteria for female competition must consider male development rather than relying on current testosterone levels. Female athletes should be recognized as the key stakeholders in the consultation and decision-making processes. We urge the IOC to reevaluate the recommendations of their Framework to include a comprehensive understanding of the biological advantages of male development to ensure fairness and safety in female sports.

Monitoring of simulated clandestine graves of victims using UAVs, GPR, electrical tomography and conductivity over 4-8 years post-burial to aid forensic search investigators in Colombia, South America.

In Colombia there are estimated to be over 121,000 missing people and victims of forced disappearances. Forensic investigators therefore need assistance in determining optimal detection techniques for buried victims, to give victims' families closure and for the wider community to see that justice is being served. Previous research has created 12 controlled simulated clandestine graves of typical Colombian murder victim scenarios at 0.5 m - 1.2 m depths in savannah and rainforest sites in Colombia. The 0-3 years of geophysical monitoring results of were published, with this paper reporting on 4-8 years monitoring of both UAV drone results and geophysical data. The UAV results from the year 8 survey, published for first time from Colombia, showed that the simulated graves could still be located using NDVI and NIR multi-spectral data, but not using optical or other multi-spectral data. The 0-3 years of geophysical data found the simulated clandestine graves could be detected with electrical resistivity and GPR methods, with the 4-8 year surveys evidencing that they could still be detected using bulk ground conductivity surveys, GPR horizontal time slice datasets and 2D ERT profiles. Research implications suggest initial use of UAV remote sensing technology to pinpoint likely search areas, before subsequent ground reconnaissance, geophysical surveys and their interpretation, before intrusive investigation methods are employed for detecting missing and disappeared persons in Colombia.

Extended reality (XR) virtual practical and educational eGaming to provide effective immersive environments for learning and teaching in forensic science.

Online virtual learning resources have been available for learning and teaching in forensic science for some years now, but the recent global COVID-19 related periods of irregular lockdown have necessitated the rapid development of these for teaching, learning and CPD activities. However, these resources do need to be carefully constructed and grounded in pedagogic theory to be effective. This article details eXtended Reality (XR) learning and teaching environments to facilitate effective online teaching and learning for forensic geoscientists. The first two case studies discussed in this article make use of Thinglink software to produce virtual learning and teaching XR resources through an internet system, which was delivered to undergraduate students in 2021. Case one details a range of XR virtual laboratory-based equipment resources, providing a consistent, reliable and asynchronous learning and teaching experience, whilst the second case study presents an XR virtual learning applied geophysics resource developed for a 12-week CPD training programme. This programme involves recorded equipment video resources, accompanying datasets and worksheets for users to work through. Both case studies were positively received by learners, but there were issues encountered by learners with poor internet connections or computer skills, or who do not engage well with online learning. A third case study showcases an XR educational forensic geoscience eGame that was developed to take the user through a cold case search investigation, from desktop study through to field reconnaissance and multi-staged site investigations. Pedagogic research was undertaken with user questionnaires and interviews, providing evidence that the eGame was an effective learning and teaching tool. eGame users highly rated the eGame and reported that they raised awareness and understanding of the use of geophysics equipment and best practice of forensic geoscience search phased investigations. These types of XR virtual learning digital resources, whilst costly to produce in terms of development time and staff resource, provide a complementary virtual learning experience to in-situ practical sessions, and allow learners to asynchronously familiarise themselves with equipment, environments and techniques resulting in more efficient use of in situ time. The XR resources also allow learners to reinforce learning post in-situ sessions. Finally, XR resources can provide a more inclusive and authentic experience for learners who cannot attend or complete work synchronously.

Portable X-ray fluorescence (pXRF) analysis of heavy metal contamination in church graveyards with contrasting soil types.

Human remains have been interred in burial grounds since historic times. Although the re-use of graveyards differs from one country, region or time period to another, over time, graveyard soil may become contaminated or enriched with heavy metal elements. This paper presents heavy metal element soil analysis from two UK church graveyard study sites with contrasting necrosols, but similar burial densities and known burial ages dating back to the sixteenth century and some possibly older than 1,000 years. Portable X-ray fluorescence element laboratory-based analyses were undertaken on surface and near-surface soil pellets. Results show elevated levels of Fe, Pb, Mn, Cr, Cu, Zn and Ca in both necrosols when compared with background values. Element concentration anomalies remained consistently higher than background samples down to 2 m, but reduced with distance away from church buildings. Element concentration anomalies are higher in the clay-rich necrosol than in sandy necrosol. Study result implications suggest that long-used necrosols are likely to be more contaminated with heavy metal elements than similar soil outside graveyards with implications for burial grounds management, adjacent populations and where burial grounds have been deconsecrated and turned to residential dwellings.

Monitoring of simulated clandestine graves of dismembered victims using UAVs, electrical tomography, and GPR over one year to aid investigations of human rights violations in Colombia, South America.

In most Latin American countries, there are significant numbers of missing people and forced disappearances, over 120,000 in Colombia alone. Successful detection of shallow buried human remains by forensic search teams is difficult in varying terrain and climates. Previous research has created controlled simulated clandestine graves of murder victims to optimize search techniques and methodologies. This paper reports on a study on controlled test site results over four simulated dismembered victims' clandestine graves as this is sadly a common scenario encountered in Latin America. Multispectral images were collected once post-burial, electrical resistivity surveys were collected 4 times, and ground penetrating radar (GPR) surveys were collected three times up to the end of the 371 day survey monitoring period. After data processing, results showed that the multispectral data set could detect the simulated clandestine and control graves, with electrical resistivity imaging relative high resistances over some of the simulated graves but not over the empty control graves. GPR results showed good imaging on the Day 8 surveys, medium imaging on the Day 294 surveys, and medium to good imaging on the Day 371 surveys. Study implications suggest that, while clandestine graves of dismembered homicide victims would likely result in smaller-sized graves when compared to graves containing intact bodies, these graves can still potentially be detected using remote sensing and geophysical methods.

Geophysical monitoring of simulated homicide burials for forensic investigations.

Finding hidden bodies, believed to have been murdered and buried, is problematic, expensive in terms of human resource and currently has low success rates for law enforcement agencies. Here we present, for the first time, ten years of multidisciplinary geophysical monitoring of simulated clandestine graves using animal analogues. Results will provide forensic search teams with crucial information on optimal detection techniques, equipment configuration and datasets for comparison to active and unsolved cold case searches. Electrical Resistivity (ER) surveys showed a naked burial produced large, low-resistivity anomalies for up to four years, but then the body became difficult to image. A wrapped burial had consistent small, high-resistivity anomalies for four years, then large high-resistivity anomalies until the survey period end. Ground Penetrating Radar (GPR) 110-900 MHz surveys showed the wrapped burial could be detected throughout. 225 MHz GPR data was optimal, but the naked burial was poorly imaged after six years. Results suggested conducting both ER and GPR surveys if the burial style was unknown when searching for interred remains. Surveys in winter and spring produced the best datasets, and, as post-burial time increases, surveying in these seasons became increasingly important. This multidisciplinary study provides critical new insights for law enforcement and families of the disappeared worldwide.

Testing Application of Geographical Information Systems, Forensic Geomorphology and Electrical Resistivity Tomography to Investigate Clandestine Grave Sites in Colombia, South America.

Colombian forensic investigators required assistance locating clandestine burials of missing persons related to human right atrocities from 14 years ago. Geoscientific search methods were trialled, including a predictive spatial statistical model, using various input and database information, to select the most likely grave locations in difficult mountainous terrain. Groundwork using forensic geomorphology, near-surface geophysics (ERT) and subsequent probing identified suspect burial positions. One site was in mountainous terrain and the other in former school grounds, both difficult to access and in poor weather conditions. In the mountainous area, a negative resistivity anomaly area was identified and intrusively investigated, found to be a buried rock. In school grounds, after MESP and intelligence were used to identify a burial site, surface depressions were identified, and ERT datasets collected over the highest priority depression; intrusive investigations discovered a hand-dug pit containing animal bones. This approach is suggested for Latin American searches.

The Search for "Fred": An Unusual Vertical Burial Case.

Police witness intelligence stated a murdered adult male "Fred" had been vertically buried in wooded hilly terrain 30 years ago in the Midlands, U.K. Conventional search methods were unsuccessful; therefore, the police requested a geophysical investigation to be undertaken to determine whether "Fred" could be detected. A multiphased geophysical approach was conducted, using bulk ground conductivity and metal detectors, then follow-up magnetics and ground penetrating radar (GPR) survey profiles on electromagnetic (EM) anomalous areas. A tight grid pattern was used to account for the reduced target size. Relatively high-resolution EM and GPR techniques were determined optimal for this terrain and sandy soil. Geophysical anomalies were identified and the most promising intrusively investigated, and this was found to be a large boulder and tree roots. Study implications suggest careful multiphase geophysical surveys are best practice and give confidence in cold case searches. This study yielded a no-body result, effectively saving police time and costs from further investigations.

Effects of an increase in intensity during tapering on 1500-m running performance.

We examined the effect of completing the final interval training session during a taper at either (i) race pace (RP) or (ii) faster than RP on 1500-m running performance and neuromuscular performance. Ten trained runners (age, 21.7 ± 3.0 years; height, 182.9 ± 7.0 cm; body mass, 73.4 ± 6.8 kg; and personal best 1500-m time, 4:17.5 ± 0:26.9 min) completed 2 conditions consisting of 7 days of regular training and a 7-day taper, separated by 3 weeks of training. In 1 condition, the taper was prescribed using prediction models based on the practices of elite British middle-distance runners, with the intensity of the final interval session being equal to 1500-m RP. The taper was repeated in the high-intensity (HI) condition, with the exception that the final interval session was completed at 115% of 1500-m RP. A 1500-m treadmill time trial and measures of maximal voluntary contraction (MVC) and rate of force development (RFD) were completed before and after regular training and tapering. Performance was most likely improved after RP (mean ± 90% confidence limits, 10.1 ± 1.6 s), and possibly beneficial after HI (4.2 ± 12.0 s). Both MVC force (p = 0.002) and RFD (p = 0.02) were improved after tapering, without differences between conditions. An RP taper based on the practices of elite middle-distance runners is recommended to improve performance in young, subelite runners. The effect of this strategy with an increase in interval intensity is highly variable and should be implemented with caution.

Altitude training in endurance running: perceptions of elite athletes and support staff.

This study sought to establish perceptions of elite endurance athletes on the role and worth of altitude training. Elite British endurance runners were surveyed to identify the altitude and hypoxic training methods utilised, along with reasons for use, and any situational, cultural and behaviour factors influencing these. Prior to the 2012 Olympics Games, 39 athletes and 20 support staff (coaches/practitioners) completed an internet-based survey to establish differences between current practices and the accepted "best-practice". Almost all of the athletes (98%) and support staff (95%) surveyed had utilised altitude and hypoxic training, or had advised it to athletes. 75% of athletes believed altitude and hypoxia to be a "very important" factor in their training regime, with 50% of support staff believing the same. Athletes and support staff were in agreement of the methods of altitude training utilised (i.e. 'hypoxic dose' and strategy), with camps lasting 3-4 weeks at 1,500-2,500 m being the most popular. Athletes and support staff are utilising altitude and hypoxic training methods in a manner agreeing with research-based suggestions. The survey identified a number of specific challenges and priorities, which could provide scope to optimise future altitude training methods for endurance performance in these elite groups.

Wildlife crime: The application of forensic geoscience to assist with criminal investigations.

Wildlife crime is a growing problem in many rural areas. However, it can often be difficult to determine exactly what had happened and provide evidential Court material, especially where evidence is ephemeral. This paper presents a case study where a badger sett had been illegally filled and evidence was rapidly required to support a prosecution before it was either destroyed by the suspect/further badger activities or eroded by weather/time. A topographic surface survey was undertaken, quantifying the number and spatial position of sett entrances, as well as which had been filled by a slurry material. A ground penetrating radar survey was also undertaken to quantify how much tunnels were filled. Study results evidenced five sett tunnels were filled out of twelve observed. The slurry fill material was not being observed elsewhere on the surface. GPR survey data evidenced ∼1m-5m of slurry fill in tunnels. A subsequent report was forwarded to the CPS as evidential material. Study implications suggest the importance of rapid geoscience surveys to assist Police Forces to both gain scientific evidence for prosecutions and to deter future wildlife crime.

Inorganic elemental analysis of decomposition fluids of an in situ animal burial.

In homicide investigations, it is critically important that post-mortem (PMI) and post-burial interval (PBI) of buried victims are determined accurately. However, clandestine graves can be difficult to locate; and the detection rates for a variety of search methods can be very low. This paper presents elemental analysis results of 18 months of decomposition fluids from an in situ buried animal cadaver used as a human clandestine burial proxy. Study results showed potassium, sulphate and sodium are key detectable elements which mirror observed conductivity temporal changes from this and other studies. Seasonal rainfall has a strong influence on both fluid generation and subsequent concentration which needs to be accounted for. Study implications suggest inorganic elements could provide both detection and potential dating of discovered clandestine burials.

Torque, power and muscle activation of eccentric and concentric isokinetic cycling.

This study aimed to establish the effect of cycling mode and cadence on torque, external power output, and lower limb muscle activation during maximal, recumbent, isokinetic cycling. After familiarisation, twelve healthy males completed 6 × 10 s of maximal eccentric (ECC) and concentric (CON) cycling at 20, 40, 60, 80, 100, and 120 rpm with five minutes recovery. Vastus lateralis, medial gastrocnemius, rectus femoris, and biceps femoris surface electromyography was recorded throughout. As cadence increased, peak torque linearly decreased during ECC (350-248 N·m) and CON (239-117 N·m) and peak power increased in a parabolic manner. Crank angle at peak torque increased with cadence in CON (+13°) and decreased in ECC (-9.0°). At all cadences, peak torque (mean +129 N·m, range 111-143 N·m), and power (mean +871 W, range 181-1406 W), were greater during ECC compared to CON. For all recorded muscles the crank angle at peak muscle activation was greater during ECC compared to CON. This difference increased with cadence in all muscles except the vastus lateralis. Additionally, peak vastus laterallis and biceps femoris activation was greater during CON compared to ECC. Eccentric cycling offers a greater mechanical stimulus compared to concentric cycling but the effect of cadence is similar between modalities. Markers of technique (muscle activation, crank angle at peak activation and torque) were different between eccentric and concentric cycling and respond differently to changes in cadence. Such data should be considered when comparing between, and selecting cadences for, recumbent, isokinetic, eccentric and concentric cycling.

GPR and ERT detection and characterization of a mass burial, Spanish Civil War, Northern Spain.

Around 27,000 people were killed in the province of Asturias during the Spanish Civil War, with several thousands killed after the war ended. There are currently over 2,000 known mass burial locations throughout Spain, but many more are unknown. Geophysics is a useful tool employed to help in the active attempts to document and improve knowledge about victims from this conflict. This paper details a non-invasive study of the Cementerio de El Salvador, in the city of Oviedo, Northern Spain. Part of the cemetery contains a known mass burial with approximately 1,300 individuals from the Spanish Civil War and post-war repression eras. Multi-frequency near-surface geophysical techniques were undertaken, after permission, to enhance knowledge about which, if any, techniques should be used to detect, delineate and analyse such mass graves. Multi-frequency (250MHz and 500MHz) ground-penetrating radar surveys were acquired together with 2D and 3D Electrical Resistivity Tomography datasets. The results have established the limits of the mass grave and improve the knowledge of the internal mass grave structure. The paper also shows the importance of considering the climatic conditions during data acquisition. This has important implications for the successful detection of recent historical mass burials using near-surface geophysics.

Long-term Geophysical Monitoring of Simulated Clandestine Graves using Electrical and Ground Penetrating Radar Methods: 4-6 Years After Burial.

This ongoing monitoring study provides forensic search teams with systematic geophysical data over simulated clandestine graves for comparison to active cases. Simulated "wrapped," "naked," and "control" burials were created. Multiple geophysical surveys were collected over 6 years, here showing data from 4 to 6 years after burial. Electrical resistivity (twin electrode and ERI), multifrequency GPR, grave and background soil water were collected. Resistivity surveys revealed that the naked burial had low-resistivity anomalies up to year four but then difficult to image, whereas the wrapped burial had consistent large high-resistivity anomalies. GPR 110- to 900-MHz frequency surveys showed that the wrapped burial could be detected throughout, but the naked burial was either not detectable or poorly resolved. 225-MHz frequency GPR data were optimal. Soil water analyses showed decreasing (years 4 to 5) to background (year 6) conductivity values. Results suggest both resistivity and GPR surveying if burial style unknown, with winter to spring surveys optimal and increasingly important as time increases.

Discovery of a mass grave from the Spanish Civil War using Ground Penetrating Radar and forensic archaeology.

An estimated 500,000 people died from all causes during the Spanish Civil War between 1936 and 1939, with a further 135,000 killed after the war ended. There are currently over 2000 known mass burial locations throughout Spain but many more are unknown. This study details the successful search for an unmarked mass grave in mountainous terrain in the Asturias region of Northern Spain. Two approximate locations were known due to eyewitness accounts. A phased site investigation approach was undertaken, which included Ground Penetrating Radar. Results showed a clear geophysical anomaly on 2D GPR profiles. The identified area was subsequently intrusively investigated by forensic archaeologists and human remains were successfully discovered. Careful and sensitive investigations are essential in these approaches where living relatives are involved.

Exercise duration-matched interval and continuous sprint cycling induce similar increases in AMPK phosphorylation, PGC-1α and VEGF mRNA expression in trained individuals.

PURPOSE: The effects of low-volume interval and continuous 'all-out' cycling, matched for total exercise duration, on mitochondrial and angiogenic cell signalling was investigated in trained individuals. METHODS: In a repeated measures design, 8 trained males ([Formula: see text], 57 ± 7 ml kg(-1) min(-1)) performed two cycling exercise protocols; interval (INT, 4 × 30 s maximal sprints interspersed by 4 min passive recovery) or continuous (CON, 2 min continuous maximal sprint). Muscle biopsies were obtained before, immediately after and 3 h post-exercise. RESULTS: Total work was 53 % greater (P = 0.01) in INT compared to CON (71.2 ± 7.3 vs. 46.3 ± 2.7 kJ, respectively). Phosphorylation of AMPK(Thr172) increased by a similar magnitude (P = 0.347) immediately post INT and CON (1.6 ± 0.2 and 1.3 ± 0.3 fold, respectively; P = 0.011), before returning to resting values at 3 h post-exercise. mRNA expression of PGC-1α (7.1 ± 2.1 vs. 5.5 ± 1.8 fold; P = 0.007), VEGF (3.5 ± 1.2 vs. 4.3 ± 1.8 fold; P = 0.02) and HIF-1α (2.0 ± 0.5 vs. 1.5 ± 0.3 fold; P = 0.04) increased at 3 h post-exercise in response to INT and CON, respectively; the magnitude of which were not different between protocols. CONCLUSIONS: Despite differences in total work done, low-volume INT and CON 'all-out' cycling, matched for exercise duration, provides a similar stimulus for the induction of mitochondrial and angiogenic cell signalling pathways in trained skeletal muscle.

Geophysical monitoring of simulated graves with resistivity, magnetic susceptibility, conductivity and GPR in Colombia, South America.

In most Latin American countries there are significant numbers of both missing people and forced disappearances, ∼71,000 Colombia alone. Successful detection of buried human remains by forensic search teams can be difficult in varying terrain and climates. Three clandestine burials were simulated at two different depths commonly encountered in Latin America. In order to gain critical knowledge of optimum geophysical detection techniques, burials were monitored using: ground penetrating radar, magnetic susceptibility, bulk ground conductivity and electrical resistivity up to twenty-two months post-burial. Radar survey results showed good detection of modern 1/2 clothed pig cadavers throughout the survey period on 2D profiles, with the 250MHz antennae judged optimal. Both skeletonised and decapitated and burnt human remains were poorly imaged on 2D profiles with loss in signal continuity observed throughout the survey period. Horizontal radar time slices showed good anomalies observed over targets, but these decreased in amplitude over the post-burial time. These were judged due to detecting disturbed grave soil rather than just the buried targets. Magnetic susceptibility and electrical resistivity were successful at target detection in contrast to bulk ground conductivity surveys which were unsuccessful. Deeper burials were all harder to image than shallower ones. Forensic geophysical surveys should be undertaken at suspected burial sites.

Soilwater Conductivity Analysis to Date and Locate Clandestine Graves of Homicide Victims.

In homicide investigations, it is critically important that postmortem interval and postburial interval (PBI) of buried victims are determined accurately. However, clandestine graves can be difficult to locate; and the detection rates for a variety of search methods (ranging from simple ground probing through to remote imaging and near-surface geophysics) can be very low. In this study, simulated graves of homicide victims were emplaced in three sites with contrasting soil types, bedrock, and depositional environments. The long-term monthly in situ monitoring of grave soil water revealed rapid increases in conductivity up to 2 years after burial, with the longest study evidencing declining values to background levels after 4.25 years. Results were corrected for site temperatures and rainfall to produce generic models of fluid conductivity as a function of time. The research suggests soilwater conductivity can give reliable PBI estimates for clandestine burials and therefore be used as a grave detection method.