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PURPOSE: The purpose of this study was to compare the effectiveness of P11-4 self-assembling peptide (CurodontTM Repair [CR]) and fluoride varnish with xylitol-coated calcium phosphate (Embrace TM Varnish [EV]) on enamel permeability and in the management of white spot lesions (WSLs) in primary teeth. METHODS: A clinical trial was conducted among 30 children aged three to five years with WSLs in 60 anterior teeth. They were randomly assigned to receive CR or EV. Preintervention and postintervention evaluation was done by International Caries Detection and Assessment System (ICDAS) and morphometric analysis. The secondary outcome was to assess the enamel permeability using scanning electron microscopy (SEM) of polyvinyl siloxane impressions. RESULTS: A statistically significant reduction in the ICDAS scores (P=0.05) and percentage area of WSLs in morphometric analysis (P=0.008) was seen in the CR group after six months. No statistically significant difference was observed in the EV group after six months. The SEM evaluation did not show a significant reduction in the percentage area of droplets in both the CR and EV groups (P=0.06 and P=0.21, respectively). No significant difference was seen between EV and CR in the three parameters assessed. CONCLUSION: Curodont TM Repair is effective at remineralizing white spot lesions in primary teeth and can be considered as a remineralizing agent.
Subject(s)
Dental Caries , Tooth Remineralization , Child , Child, Preschool , Humans , Cariostatic Agents , Dental Caries/drug therapy , Dental Caries/pathology , Fluorides , Microscopy, Electron, ScanningABSTRACT
Identification of BCR-ABL1 fusion gene amplification status is critically important in the effective management of chronic myelogenous leukemia (CML) patients. Earlier reports suggested that overexpression of BCR-ABL1 either through amplification of BCR-ABL1 fusion gene or by the up regulation of BCR-ABL1 transcript level might be an early phenomenon in the establishment of IM resistance and disease evolution in CML. In the current study, we performed dual color dual fusion locus specific BCR/ABL1 FISH analysis along with karyotype analysis using GTG banding (G-banding using trypsin and Giemsa) technique in 489 patients with different clinical stages of CML at diagnosis or during the course of the disease to unravel the spectrum of BCR-ABL1 fusion gene amplification status. Among the study group analyzed, it was found that prevalence of occurrence of BCR-ABL1 fusion gene amplification was significantly higher in advanced stages of disease and in IM resistant CML-CP patients when compared to initial stage of disease, de novo CML-CP. Cytogenetic and metaphase FISH characterization on our study samples revealed that BCR-ABL1 fusion gene amplification was occurred through the formation of extra copies Ph chromosomes and isoderived Ph chromosomes. Current study suggests that unrestrained activity of BCR-ABL1 played a vital role in resistance to targeted therapy and disease evolution in CML. In our study population, patients in progressive stage CML and in IM resistant CP with multiple copies of BCR-ABL1 fusion gene displayed a poor response to targeted treatment with IM. Hence, the early identification of BCR-ABL1 fusion gene amplification using FISH technique will lead to improved interventions and outcome in future CML patients.
Subject(s)
Fusion Proteins, bcr-abl , Gene Amplification , Leukemia, Myelogenous, Chronic, BCR-ABL Positive , Philadelphia Chromosome , Adolescent , Adult , Aged , Aged, 80 and over , Chromosome Banding , Female , Fusion Proteins, bcr-abl/genetics , Fusion Proteins, bcr-abl/metabolism , Humans , In Situ Hybridization, Fluorescence , Leukemia, Myelogenous, Chronic, BCR-ABL Positive/enzymology , Leukemia, Myelogenous, Chronic, BCR-ABL Positive/genetics , Leukemia, Myelogenous, Chronic, BCR-ABL Positive/mortality , Leukemia, Myelogenous, Chronic, BCR-ABL Positive/therapy , Male , Middle AgedABSTRACT
AIM: To evaluate the antimicrobial efficacy of diode laser, triphala, and sodium hypochlorite (NaOCl) against Enterococc-cus faecalis contaminated primary root canals. MATERIALS AND METHODS: Forty-nine single-rooted human primary teeth were reduced up to cemento-enamel junction and biomechanically prepared. After sterilization, five teeth were selected as negative controls and remaining teeth were inoculated with E. faecalis. The teeth were then randomly divided into four groups. The first group was irradiated with diode laser, the second group was irrigated with sodium hypochlorite, and the third group with triphala solution. The fourth group served as the positive control. The antimicrobial efficacy was tested by collecting transfer fluid saline from the canals and counting the colony forming units (CFUs) of viable E. faecalis on agar plates. The Mann-Whitney test was used to analyze the results, using Statistical Package for the Social Sciences software version 19. RESULTS: The results showed that mean bacterial CFU were 8.00 ± 7.87 for laser, 58.60 ± 16.63 for triphala, and 69.80 ± 19.57 for NaOCl. Laser group showed significant reduction in the colony count compared to the other groups. Triphala group showed better antibacterial activity than NaOCl, but the difference was not statistically significant. CONCLUSION: Laser was most effective against E. faecalis and triphala can be used as an alternative disinfectant to NaOCl in primary root canals. HOW TO CITE THIS ARTICLE: Thomas S, Asokan S, John B, Priya G, Kumar S. Comparison of Antimicrobial Efficacy of Diode Laser, Triphala, and Sodium Hypochlorite in Primary Root Canals: A Randomized Controlled Trial. Int J Clin Pediatr Dent 2017;10(1):14-17.
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OBJECTIVES: The objective of the study was to assess the behavioral effects and the changes in the anxiety level of children after intranasal and sublingual midazolam sedation using Venham's clinical anxiety scale and salivary cortisol level. STUDY DESIGN: Twenty children aged 3 to 7 years were randomly assigned to Group A (n=10) intranasal or Group B (n=10) sublingual midazolam (0.2mg/kg) sedation. The anxiety levels at various time periods were assessed using Venham clinical anxiety scale and corresponding changes in salivary cortisol levels were assessed before and after the drug administration. The anxiety levels were assessed independently by two pediatric dentists from recorded videos. Wilcoxon signed rank test and Mann Whitney U test were used for statistical analysis using SPSS version 19.0. RESULTS: There was a significant decrease in anxiety level from baseline to 20 minutes after drug administration in group A (p=0.004) and group B (p=0.003). There was no significant change in salivary cortisol levels before and after the drug administration in group A (p=0.07) and group B (p=0.38). CONCLUSIONS: Both intranasal and sublingual administration of midazolam was equally effective in reducing the child's anxiety. However, there was no significant difference in the salivary cortisol levels in both groups.
Subject(s)
Child Behavior/drug effects , Conscious Sedation/methods , Dental Anxiety/prevention & control , Hypnotics and Sedatives/administration & dosage , Midazolam/administration & dosage , Administration, Intranasal , Administration, Sublingual , Aerosols , Anesthetics, Local/administration & dosage , Child , Child, Preschool , Dental Restoration, Permanent/methods , Humans , Hydrocortisone/analysis , Lidocaine/administration & dosage , Pulpectomy/methods , Pulpotomy/methods , Saliva/chemistry , Single-Blind Method , Tooth Extraction/methodsABSTRACT
INTRODUCTION: "The fibers running anteroposteriorly within the core and in concentric curves across the base of each ruga" determine their orientation and forms. The varying shapes of palatal rugae can be attributed to the fact that rugae develop as localized regions of epithelial proliferation and thickening. Fibroblasts and collagen fibers then accumulate in the connective tissue beneath the thickened epithelium and assume distinct orientation. AIM: The aim of the present study was to assess the morphology, gender difference of rugae pattern in 5-15 year old children. MATERIALS AND METHODS: The various diagnostic dental stone cast available in Department of Pedodontics were analyzed by the method based on Thomas and Kotze classification in 1983. RESULTS: There was a female prediction in the total count and primary rugae pattern. Comparing the shapes of rugae both in male and female study models showed a predominance in wave shape followed by cure. No circular pattern was observed in the study population. No statistical difference in the direction and unification of rugae among males and females. CONCLUSION: The fingerprint-like uniqueness of rugae to each individual has become accepted as a possible aid to person identification. This may help narrow the field for identification and give results in conjunction with the other methods such as visual, fingerprints, and dental characteristics in forensic sciences.
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Oligodontia is one of the most common developmental abnormalities in humans. The present case report highlights the features of oligodontia in a 12-year-old male patient which was managed successfully with multidisciplinary approach. Familial oligodontia represents as an absence of varying numbers of secondary teeth seen as an isolated trait. The advance in the understanding of tooth development and genetic control of tooth morphology not only allows clinical research to broaden the knowledge of tooth agenesis but also provides optimum clinical care.
