ABSTRACT
Background: The topical fluoride acts on the tooth in many ways and their most important action is inhibition of demineralization and enhancement of enamel remineralization. Aim: The purpose of this clinical trial was to assess the fluoride concentration in saliva before and after 38% silver diamine fluoride (SDF) and 5% sodium fluoride (NaF) application on enamel and duration of its availability at different time intervals. Methodology: A randomized clinical trial was conducted among 40 healthy children aged between 6 and 12 years. The participants were then randomly allocated into two different groups in which the first group (group I) was given 30% SDF and the second group (group II) were topically applied with 5% NaF solution. The fluoride concentration was measured in the salivary samples, which were collected at three time intervals, that is, at baseline (S1), 2 hours (S2), and 24 hours (S3) after application. Analysis of variance (ANOVA) test was used for evaluation and independent paired t-test was conducted for comparison between groups. Results: When using an ANOVA with repeated measures with a Greenhouse-Geisser correction, the mean scores of fluoride concentration were statistically significantly different at different time intervals for both NaF (F = 20.854, p < 0.0005) and SDF (F = 22.746, p < 0.0005). Conclusion: The present trial concluded that topical fluoride application increases fluoride bioavailability in saliva thereby increasing tooth remineralization. A steep rise in fluoride concentration was observed shortly post-SDF application at 2 hours and 24 hours time interval emerging a need for further research into the field of fluoridation with SDF. How to cite this article: Jabin Z, Nasim I, Priya V V, et al. Comparative Evaluation of Salivary Fluoride Concentration after Topical Application of Silver Diamine Fluoride and Sodium Fluoride: A Randomized Controlled Trial. Int J Clin Pediatr Dent 2022;15(3):371-375.
ABSTRACT
Fibrodysplasia ossificans progressiva (FOP) is a rare genetic disorder which is autosomal dominant distinguished by congenital malformations of large toes and flare ups, etc. It is a disorder of connective tissue, with heterotopic ossifications seen with skeletal muscles, tendons, and cartilages and also called as Stone man disease, myositis ossificans, and Munchmeyer disease. The main objective of the study is to assess and create the awareness about FOP among dental students. An online-based survey was conducted among 103 dental students, undergraduates and postgraduates. About 20 questionnaires were prepared and circulated among the students through the "Google forms" across Chennai. A survey questionnaire asked about the knowledge of fibrodysplasia, its causes, treatment, and diagnosis was enquired. Thus, the data obtained were analyzed statistically using the SPSS software. In our study, about 64% were undergraduates and 35% of them were postgraduates. About 66% of the participants were aware of fibrodysplasia and 32% of them were not aware. Most of the undergraduate students were more aware of fibrodysplasia (40%) when compared to postgraduates (29%). However, this is statistically not significant. Fibrodysplasia ossificans reported to have a high incidence in 1 in 2 million people worldwide. Hence, an early diagnosis of this disorder can prevent further complications. Although the survey has provided significant knowledge about fibrodysplasia, awareness still has to be created among dental students.
ABSTRACT
Chronic bronchitis (CB) is an inflammatory disease and is characterized by bronchial tube irritation. The bronchial tube irritation can result in the accumulation of mucus. CB is a part of chronic obstructive pulmonary disease, causing blockage of airflow and thereby problems in breathing. Cigarette smoking is of utmost importance in causing CB. Besides pollution of air and the working environment can also play a key role. The study aimed to assess and create the awareness about CB among dental students and practitioners. A cross-sectional questionnaire survey was conducted among dental students utilizing an online review entry called "Google forms." A total of 100 people were assessed using a structured questionnaire comprising 15 questions. The analysis of the result was done using the SPSS software of version 23. It has been shown that 78% of them are aware of CB. 75% of them responded that passive smokers were mostly affected by CB. Within the study limits, it was found that males who participated had increased awareness when compared to the females. The survey results showed that most of the participants were aware about CB but not its clinical features, oral manifestations, diagnosis, and treatment.
ABSTRACT
BACKGROUND: The purpose of this study was to identify the genetic patterns of Streptococcus mutans by DNA finger printing among caries-active, caries-free, and in children with black tooth stains. MATERIALS AND METHODS: In this in vitro study Ten children with black tooth stains and ten caries-free and caries-active children between 3 and 6 years of age were recruited. Saliva samples were collected using a sterile tube, followed by microbial culture of S. mutans, DNA isolation, and polymerase chain reaction amplification. The molecular weights of each band were converted into binary data, and data were entered into SPSS to generate similarity dendrograms. RESULTS: Dendrogram interpretation of black tooth stain and caries-free samples revealed an identical genetic pattern in 15 samples with high genetic similarity. Dendrogram interpretation of black tooth stain and caries-active samples exhibited a greater genetic diversity. CONCLUSION: This study concludes that identical genotypes and high genetic similarity among isolates of the caries-free and black tooth stains. Polymorphism and genetic diversity exist between the black tooth stain and caries-active samples.
ABSTRACT
OBJECTIVES: At present, there is lack of evidence in literature regarding the transmission of Streptococcus Mutans from a caries-free mother to child by genetic methods, so the present study aimed to identify the genetic characterization of Streptococcus Mutans strains isolated from caries-free and caries active children and their mother being caries-free in both the groups and also to identify the genetic patterns of Streptococcus Mutans between caries-free and caries-active individuals. METHODS: Twenty child-mother pair were selected and divided into 2 groups and the mothers being caries-free in both the groups. Saliva samples were collected using a sterile tube, followed by microbial culture of Streptococcus mutans, DNA isolation and PCR amplification. The molecular weights of each band were converted into a binary data and data were entered into SPSS software version 20.0 to generate similarity dendrograms. RESULTS: Amplified products of Streptococcus Mutans demonstrated a same genetic distance between the mother-child pair, indicative of a closely related species. Dendrogram interpretation represented a greater genetic polymorphism of Streptococcus Mutans between caries-free and caries-active children. CONCLUSIONS: Identical genetic distance between child-mother pair showed that, these Streptooccus Mutans were closely related and could have vertically transmitted from their mothers. Different genotypic pattern between caries-free and caries active subjects showed a genetic polymorphism among the Streptococcus Mutans strains.
ABSTRACT
BACKGROUND: Garcinia mangostana is extensively used in most of the Indian herbal pharmaceuticals and nutraceuticals. OBJECTIVE: The objective of this study was to elucidate the underlying biochemical protective mechanism of G. Mangostana Linn. fruit extract (GME) in deterioration of diethylnitrosamine (DEN)-induced hepatic carcinoma (HCC) in rats. MATERIALS AND METHODS: The cancer was induced using DEN to the experimental rats and treated with GME (200, 400, and 600 mg/kg) to find its anticancer property. The cancer biomarkers such as alpha-fetoprotein (AFP), carcinoembryonic antigen (CEA), hepatic hydroxyl proline, total tissue protein, and tumor necrosis factor-alpha levels were measured using ELISA. The vascular endothelial growth factor expressions were also seen in liver tissues using immunohistochemistry. RESULTS: In addition, there was a significant increase in serum AFP, CEA, hepatic hydroxylproline, and total tissue protein levels in HCC group versus the negative control group. In contrast, the groups with HCC subjected to either high or low dose of GME elicited significant reduction of AFP, CEA, hepatic hydroxylproline, and increase in total protein in serum compared to the untreated HCC rats. Interestingly, treatment with GME elicited marked improvement in the liver histological feature and downregulation of tumor necrosis factor-alpha levels in HCC groups. GME extract may have chemopreventive benefits by reducing the tumor promoting growth factor levels in HCC-induced group. CONCLUSION: To sum up, all findings on curative groups had proved clearly that the GME has anticarcinogenic effect on the development of liver cancer induced by DEN in rats. SUMMARY: Garcinia mangostana Linn. (GME) may have chemopreventive property by reducing the tumor promoting growth factor and tumor necrosis factor-alpha levels in diethylnitrosamine (DEN)-induced hepatic cancerThe suppression in the levels of cancer biomarkers by GME due to the presence of anticancer phytoconstituentsThe histological studies proved the effective dose of GME against DEN-induced experimental hepatic cancer. Abbreviations used: TNF-α: Tumor necrosis factor-alpha, PBS: Phosphate buffered saline, ROS: Reactive oxygen species, DNA: Deoxyribonucleic acid, GSH: Glutathione, VEGF: Vascular endothelial growth factor.