Free Gingival Grafting of Single Tooth Recession with Advanced-Platelet-Rich Fibrin as a Palatal Bandage: A 12-Month Follow-Up.

Although techniques such as subepithelial connective tissue graft are now widely used for root coverage procedures, free gingival graft (FGG) continues to be a common mucogingival procedure used to increase keratinized tissue dimensions. However, the palatal donor site heals with secondary intention and requires a longer healing time causing more discomfort and pain to the patient. A healthy 22-year-old female patient presented with Miller's class II recession in relation to the tooth 31 with high frenal attachment and a shallow vestibule, which was treated using FGG, and the donor site was bandaged with advanced-platelet-rich fibrin (A-PRF). This report evaluates the healing of the donor site over a 12-month period and assesses the root coverage as well as the postoperative discomfort after the harvesting of graft. In terms of healing, the use of A-PRF membrane as a palatal bandage appears to accelerate healing at the donor site, thereby reducing postoperative complications.

In vivo study on the release of nickel, chromium, and zinc in saliva and serum from patients treated with fixed orthodontic appliances.

BACKGROUND: Fixed orthodontic appliances can release metal ions such as nickel, chromium, and zinc into saliva and blood, which can cause contact dermatitis, hypersensitivity, and cytotoxicity. This study was undertaken to assess the release of nickel, chromium, and zinc in saliva and serum of patients undergoing fixed orthodontic treatment. MATERIALS AND METHODS: This in vivo study was conducted on 80 participants with an age range of 15-40 years. Thirty were included as controls and 50 participants were treated with fixed orthodontic appliances. Saliva and blood samples were collected at five different periods, before insertion of fixed orthodontic appliance and at 1 week, 3 months, 1 year, and 1.5 years after insertion of appliance, respectively. The metal ion content in the samples were analyzed by atomic absorption spectrophotometry. Mean levels of nickel, chromium, and zinc in saliva and serum were compared between groups using independent sample t-test and before and after results using paired t-test. P < 0.05 was considered as statistically significant. RESULTS: At the end of 1.5 years, the mean salivary levels of nickel, chromium, and zinc in controls were 5.02 ppb, 1.27 ppb, and 10.24 ppb, respectively, as compared to 67 ppb, 30.8 ppb, and 164.7 ppb at the end of 1.5 years. This was statistically significant with P < 0.001. A significant increase in the metal ion levels were seen in participants with before and after insertion of appliance (P < 0.001). CONCLUSION: Orthodontic appliances do release considerable amounts of metal ions such as nickel, chromium, and zinc in saliva and serum. However, it was within permissible levels and did not reach toxic levels.

Assessment of antimicrobial activity of different concentrations of Tinospora cordifolia against Streptococcus mutans: An in vitro study.

BACKGROUND: The antimicrobial property of Tinospora cordifolia has been tested against a variety of microorganisms in the literature. The present study aimed to assess the antimicrobial activity of different concentrations of commercially available T. cordifolia powder against Streptococcus mutans. MATERIALS AND METHODS: An in vitro study was undertaken in which extract of T. cordifolia was obtained using 100% ethanol by maceration. Seven different concentrations were prepared and tested against S. mutans in brain-heart infusion agar medium. Plates were incubated aerobically at 37°C for 48 h, and zone of inhibition was measured using Vernier caliper. 0.2% chlorhexidine and dimethylformamide were used as positive and negative controls respectively. The data were analysed by descriptive analytic tests. RESULTS: The maximum antibacterial activity of T. cordifolia was observed with a volume of 40 µl at 2% concentration with a zone of inhibition of 19 mm. A 30 µl volume of 0.2% chlorhexidine showed a zone of inhibition of 28 mm, and no zone of inhibition was observed with dimethylformamide. CONCLUSION: Tinospora exhibited antimicrobial activity against S. mutans. However, it needs to be confirmed further with in vivo studies.

Effect of dried sunflower seeds on incisal edge abrasion: A rare case report.

Tooth surface loss (TSL) is a complex phenomenon characterized by the loss of hard tooth structure at various locations of the teeth, usually due to more than one factor. TSL due to abrasion can be significant in patients consuming coarse, abrasive diet. The present case reports an interesting incisal edge abrasion in a female patient, attributed to a particular dietary behavior of long-term consumption of sunflower seeds. All her family members and most of the people from her native place were also reported to have similar lesions by the patient. Larger epidemiological studies to assess the prevalence and severity of such abrasive lesions in geographic areas with this particular dietary habit need to be carried out so that people may be made aware and educated about alternative ways of eating sunflower seeds that will not cause any form of tooth wear.

Assessment of maternal risk indicators for the development of caries in their children: A comparative, cross-sectional study.

INTRODUCTION: Certain factors in mothers could be risk indicators for certain diseases in their children. Hence, this study was undertaken to assess maternal risk indicators for the development of early childhood caries in their children. METHODOLOGY: A comparative, cross-sectional study was designed among 180 mother-child pairs attending various Anganwadi centers. Demographic, dietary, oral hygiene practices and other necessary information were collected from mothers using a structured questionnaire. Caries status and amount of plaque were recorded through clinical examination. Nonstimulated saliva from mothers was cultured for mutans streptococci (MS). Data were analyzed using SPSS version 17. Chi-square, Student's t-test, and logistic regression were used. A P ≤ 0.05 was considered statistically significant. RESULTS: In the study group, 73.3% of mothers had caries as compared to only 53.3% mothers in control group. While mean DMFT and mean DMFS of mothers in the study group was 3.78 ± 3.91 and 8.37 ± 12.2, respectively, the same for the mothers in the control group was 2.66 ± 3.01 and 5.8 ± 5.3. Sixty (66.7%) out of ninety mothers in the study group had a high MS count as compared to only 40 (44.4%) mothers in control group (P = 0.003). CONCLUSION: The present study showed that high salivary MS count and decay in mothers could be important risk indicators for the development of caries in their children.

Root Coverage of Palatal Recession Using Epithelial Embossed Connective Tissue: A Case Report.

Labial and buccal gingival recession is a common finding in the adult population, which can occur due to various factors. When present, it can lead to an unesthetic appearance and can cause hypersensitivity. However, very limited literature is available regarding the incidence of palatal gingival recession. Many times it goes unnoticed during routine clinical examinations. Although there are no esthetic concerns in these regions, recession can cause sensitivity and pose a technical challenge in surgical correction because of poor accessibility and visibility. This case reports the treatment of a single palatal recession defect using an epithelial embossed connective tissue graft. The 12-month follow-up of the case showed significant root coverage (93.6%) and decreased sensitivity. The advantage of the harvested graft was that the connective tissue was not exposed, as it was tucked into the underlying flap, and the mucosal contour was maintained.

Tobacco use among high school children in Bangalore, India: A study of knowledge, attitude and practice.

INTRODUCTION: Tobacco use among school children is becoming a serious problem in developing countries. The early age of initiation underscores the urgent need to intervene and protect this vulnerable group from becoming victims of this addiction. AIM: To assess the knowledge, attitudes, and practices about tobacco use among 13-15 year old school children of Bangalore City. MATERIALS AND METHODS: A cross-sectional study was designed and data on tobacco usage was collected from 1288 students aged 13-15 years studying in six government and private schools of Bangalore using a self-administered closed ended questionnaire. Data was analyzed using SPSS 15.0 and descriptive statistics was applied. Chi-square tests were used to determine the significant differences in the variables of interest. RESULTS: Out of 1288 children, 1281 (99.5%) children had heard about tobacco and 1162 (90.2%) students knew the harmful effects of tobacco. Only 28 (2.2%) had used tobacco products. Peer pressure was the main reason for tobacco use among children and age was not a barrier in buying tobacco products. Television (58%) was the main source of information for tobacco products followed by newspapers (26%) and movies (16%). CONCLUSION: It is encouraging to find that majority of the 13-15 year old children surveyed in the present study did not use tobacco and were aware of the health risks associated with tobacco use. This calls for the school authorities to be included in stricter implementation and monitoring of the implementation of legislation. Regular and systematic education programs catering to teachers, children, and also their parents should be undertaken.

Association between maternal - child levels of salivary Mutans Streptococci and early childhood caries.

BACKGROUND: To find out if there is an association between levels of salivary Mutans Streptococci (MS) of mothers and salivary MS and Caries experience of their Children. MATERIALS AND METHODS: A cross sectional study was designed among 180 mother-child pair attending Vanivilasa Children Hospital, Bangalore, India. Caries was diagnosed using WHO criteria. Ninety children with Early Childhood Caries (ECC) with their mothers were included in case group and 90 caries free children with their mothers were included in the control group. Whole non-stimulated saliva was collected from mothers and children and cultured for MS. Colonies were counted and compared. Data was analyzed using SPSS version 10. Chi-square test and t-test were used to find differences between groups. P value of <0.05 was considered as significant. RESULTS: Among 180 children, 80 of them had a high MS count. Among them, 60 (75%) had ECC and only 20 (25%) were caries free. This difference was significant with P < 0.001. Among 180 mothers, 100 had a high MS count. Among them, 60 (60%) belonged to case group and 40 (40%) to the control group (P = 0.003). Forty nine of the Children in case group with a high count also had mothers with high count as against only 14 in the control group (P < 0.001). CONCLUSION: Present study showed a strong association between maternal and child salivary MS levels indicating that mother's MS count could be an important risk indicator for ECC development.

Prevalence of early childhood caries among 3-5 year old pre-schoolers in schools of Marathahalli, Bangalore.

BACKGROUND: Dental caries among preschool children is still a major public health problem in many developing countries including India. Hence the aim of the present study was to find out the prevalence of Early Childhood Caries among 3-5 year old pre-schoolers in schools of Marathahalli, Bangalore. MATERIALS AND METHODS: A cross sectional study was conducted on 717 pre-schoolers in 6 schools of Marathahalli. Clinical examination was performed and deft index was recorded using Gruebell's criteria. Early Childhood Caries was diagnosed using Early Childhood Caries Diagnostic Criteria, consistent with the NIDCR workshop statement. Data was analysed using SPSS 15.0 and descriptive statistics was applied. Chi-square test was used to find out the significant differences. The level of significance was taken at P value < 0.05. RESULTS: Prevalence of early childhood caries was 40% with a mean deft of 1.89 (+3.3) and Significant Caries Index score was 5.51. 44.8% of 3 year old had Early Childhood Caries, 35% of 4 year old children and 41% of 5 year old had Early Childhood Caries. Almost, all of deft was due to untreated caries. CONCLUSION: The results of the present study calls for a need to focus on pre-schoolers' oral health and parental education for prevention and early detection of Early Childhood Caries. A high Significant Caries Index in this study population indicates a more targeted approach for high risk pre-schoolers.

Prevalence of early childhood caries among preschool children of low socioeconomic status in Bangalore city, India.

AIM: To assess the prevalence of Early Childhood Caries (ECC) among preschool children of low socio- economic status in Bangalore city, India. MATERIALS AND METHODS: A cross-sectional study was carried out among 566, 24-59 months old children attending various Anganwadi centers in Bangalore city, India, selected via cluster sampling. Caries experience was recorded using deft index (Greubbell, 1944). t Test, ANOVA, and Chi-square tests were used to find significant differences. P < 0.05 was considered as statistically significant. RESULTS: Prevalence of Early Childhood Caries (ECC) was 37.3% with a mean deft of 1.90 ± 3.38 ranging from 0 to 17 teeth. Out of 211 children with ECC, 94.3% had severe Early Childhood Caries (s-ECC) with a mean deft score of 5.35 ± 3.77. All of the deft was due to untreated caries. Mean caries experience of 36-47 months age group was significantly higher than other groups (P = 0.024). CONCLUSIONS: The present study showed a 37.3% prevalence of ECC. There is a need for preventive and curative oral health programs in this section of the society.

Giant intradiploic epidermoid cyst of occipital bone. A case report and literature review.

Intradiploic epidermoid cysts are congenital benign lesions. They commonly occur intracranially. Intradiploic epidermoid cyst of the calvarium is very rare and few cases have been reported to date. We describe a giant intradiploic epidermoid cyst of the occipital bone with CT and MRI characteristics including DW imaging and ADC values.

Accuracy of 64-slice coronary CT angiography in predicting percentage diameter stenosis.

OBJECTIVE: Aim of our study was to evaluate the diagnostic accuracy of 64-slice CT coronary angiogram in measuring the percentage diameter stenosis compared to invasive angiography. METHODS AND RESULTS: 100 consecutive patients with more than 50% stenosis in at least one major coronary artery measured by 64-slice CT angiogram were included in the study. Patients with atrial fibrillation, history of allergy to contrast agent, acute coronary syndrome, renal insufficiency, history of previous coronary bypass surgery or percutaneous transluminal coronary stent, heart rate more than 70 per minute at the time of scan in spite of beta-blocker therapy, and calcium score >2000 Agaston units were not included in the study. 15-segment American Heart Association classification was used, and segments were compared using qualitative angiography. 192 segments (12.80%) could not be assessed due to poor image quality. The major cause for poor image quality was dense calcification precluding the luminal assessment (60.42%). Comparing the maximal percentage diameter stenosis by 64-slice CT versus invasive angiogram, the Spearman correlation coefficient between the two modalities was 0.788 and p value was <0.001. Bland-Altman analysis showed a mean difference in percentage stenosis of 2.1 +/- 16.22%. A total of 91.97% (401 of 436) of segments were within 1.96 standard deviations. CONCLUSION: This study shows that 64-slice CT coronary angiogram is accurate in detecting percentage diameter stenosis compared to coronary angiogram if the image quality is good. Calcifications and motion artifacts are the main culprits of poor image quality.