ABSTRACT
Isoproterenol, administered intraperitoneally in rats in a dose of 2 mg/kg of body mass for a period of 20 days, induced myocardial hypertrophy. After its stoppage a period of regression occurred, during which the myocardium recovered practically to its initial state. Complex studies of connective-tissue components (collagen, glycosaminoglycans, total nonfibrillary protein, total protein, structural, glycoproteins), metrometric data-the coefficient R (1 g of myocardium/199 g of body mass) and histomorphological investigations were performed. The same studies were done on rats of a control nontreated group and of experimental groups-on the 0 day. 7th, 14th and 42 day. It was established there was a gradual recovery to normal values of collagen, total nonfibrillary protein and the coefficient R, which were highest on the day 0. Progressive diminution, e.g., normalization of both the connective tissue components and of the volume of myocardial cells was found histomorphologically, in which the changes were mostly manifested on the day 0, as well. The complex studies of the connective tissue with isoproterenol MH proved that the changes of MH in this tissue correlated with the stages of MH respectively its regression.
Subject(s)
Cardiomegaly/chemically induced , Disease Models, Animal , Isoproterenol/toxicity , Animals , Body Weight/drug effects , Cardiomegaly/metabolism , Cardiomegaly/pathology , Connective Tissue/drug effects , Connective Tissue/metabolism , Heart/drug effects , Male , Myocardium/metabolism , Organ Size/drug effects , Rats , Rats, Inbred StrainsABSTRACT
HbA2 and HbF were studied in 91 patients with different forms of psoriasis: 29 with psoriasis vulgaris placata (restricted forms); 31 with psoriasis generalisata and erythroderma psoriaticum; 21 with psoriatic arthritis; 10 with psoriasis pustulosa. Statistically significant increase of HbA2 was established in patients with psoriasis vulgaris placata (restricted forms) as well in patients with psoriatic arthritis. HbF is statistically significant increased in all forms of psoriasis. The authors suggest, that there is probably in psoriasis a disturbance in the synthesis of the globine chains, which is genetically dependent.
Subject(s)
Fetal Hemoglobin/analysis , Hemoglobin A2/analysis , Psoriasis/blood , Adult , Arthritis, Psoriatic/blood , Female , Fetal Hemoglobin/genetics , Hemoglobin A2/genetics , Humans , Male , Middle Aged , Psoriasis/geneticsABSTRACT
The character and essence of changes taking place in the connective tissue matrix of the vascular wall under the effect of thymus deficiency are not well enough clarified. The aim of the study was to assess the content of collagen, elastin, total glucosamines, total glycoproteins, total nonfibrillar protein and total protein in the aorta total glycoproteins, total nonfibrillar protein and total protein in the aorta of adult thymectomized rats of the Wistar strain and in control animals over a 12-month post-thymectomy period. The amount of collagen and elastin augments both in connection with the aging process (after 2-4 months), and as a result of thymectomy. Glycoproteins show an increase starting from the third month in the controls, and from the fifth month in thymectomized animals. Glucosamines display a fluctuating decrease parallel to aging in either of the groups under study. The content of total protein and nonfibrillar protein reveals no differences worthy of notice. It is reasonable to assume the existence of a correlation between thymus activity and metabolism of the connective tissue components in the vascular wall of rats.
Subject(s)
Aorta/metabolism , Muscle, Smooth, Vascular/metabolism , Thymus Gland/physiology , Animals , Arginine/metabolism , Collagen/metabolism , Elastin/metabolism , Glycoproteins/metabolism , Glycosaminoglycans/metabolism , Rats , Rats, Inbred Strains , Thymectomy , Tyrosine/metabolismABSTRACT
It has been demonstrated that both elastin and tropoelastin preparations obtained from aortae of spontaneously hypertensive rats at the stage of established hypertension differ in their amino acid composition from age-matching controls. The differences refer to an increased proportion of polar amino acids, particularly aspartic and glutamic acid (about a two-fold increase compared to the controls) and arginine and tyrosine (1.5 times the control value). On the other hand, this increase is compensated for by a decrease in the valine concentration. Furthermore, direct estimation of the number of val-pro sequence in different elastin preparations indicated a drop from 49.3 to 29.2 per 1,000 residues in normotensive controls and preparations obtained from spontaneously hypertensive rats respectively.
