ABSTRACT
The features of antimitotic substances as radioprotectors were studied. In vitro experiments have demonstrated that taxol revealed radioprotective features concerning the process of polymerization of irradiated microtubules. These results were the basis for the use of taxol and some other substances with high affinity for cytoskeleton proteins as potential radiomodificators in vivo. Experiments with cultivated fibroblasts revealed that colchicine significantly enhances radioactive injuries of cells while taxol and phalloidin manifest their radioprotective features.
Subject(s)
Antineoplastic Agents/therapeutic use , Radiation Injuries, Experimental/drug therapy , Radiation-Protective Agents/therapeutic use , Animals , Antineoplastic Agents/toxicity , Brain/ultrastructure , Cattle , Colchicine/therapeutic use , Colchicine/toxicity , Cytoskeletal Proteins/drug effects , Cytoskeletal Proteins/radiation effects , Drug Evaluation, Preclinical , L Cells/drug effects , L Cells/radiation effects , Mice , Microtubules/drug effects , Microtubules/radiation effects , Paclitaxel/therapeutic use , Paclitaxel/toxicity , Phalloidine/therapeutic use , Phalloidine/toxicity , Radiation Injuries, Experimental/pathology , Radiation-Protective Agents/toxicity , Time FactorsABSTRACT
The process of polymerization of microtubules isolated from bovine brain by two polymerization-depolymerization cycles has been investigated at temperatures 41 and 45 degrees C. The damages of the polymerization process using the registration of the optical solution density are shown. The electron microscopic analysis has shown the damages in the structure of microtubules formed at high temperatures.
