ABSTRACT
A use of computed express analysis of variations in RR-cardiointerval durations for patients in acute period of hemorrhagic stroke permitted to evaluate the condition severity and disease outcome prognosis before the objective changes of clinical symptomatology. A recovery was reliably observed with a positive and stable trend to U-potential and S-ULF, So, Ik indices normalization. A decrease of the indices towards a critical level was detected in cases with lethal outcome.
Subject(s)
Diagnosis, Computer-Assisted/methods , Electrocardiography/methods , Intracranial Hemorrhages/diagnosis , Stroke/diagnosis , Adult , Aged , Female , Humans , Male , Middle Aged , Prognosis , Severity of Illness IndexABSTRACT
We used restriction length polymorphism (RFLP) analysis of PCR-amplified fragments of mtDNA to study the genetic structure of chum salmon populations sampled in 1993-2000 during a spawning run in five rivers: Narva (Southern Primorye), Naiba (Sakhalin Island), Sernovodnaya (Kunashir Island, Southern Kuril Islands), Ola (northwestern coast of the Sea of Okhotsk), and Anadyr' (Chukotka Peninsula). In total, 49 haplotypes were identified in 193 fish. Heterogeneity tests showed highly significant (P = 0) differences among all sample pairs. The estimated time of independent divergence of the populations or population groups is in good agreement with the time of Pleistocene glaciations. This result suggests that it is cyclic global changes during this time period that were crucial in determining the within-species divergence in chum salmon. The types of mtDNA genetic variability and mismatch distribution between haplotypes in the populations indicate that the southern regions of the Sea of Okhotsk and Sea of Japan served as refugia for chum salmon during glaciation periods.
Subject(s)
DNA, Mitochondrial/genetics , Genetic Variation , Genetics, Population , Geography , Oncorhynchus/genetics , Phylogeny , Animals , Asia , Fresh Water , Haplotypes , Polymorphism, Restriction Fragment Length , SiberiaABSTRACT
The use of computed express analysis of RR-cardiointerval duration variability in patients with acute stage of ischemic stroke allowed to obtain the data on state dynamics and predict the disease outcome before the objective changes in clinical symptomatology. The recovery is observed in normalization of sigma, beta 1, beta 2, U, S-ULF, S-LF and S-HF indices. An opposite trend is observed in cases of lethal outcome.
Subject(s)
Diagnosis, Computer-Assisted/methods , Electrocardiography/methods , Stroke/diagnosis , Adult , Aged , Aged, 80 and over , Disease Progression , Female , Humans , Male , Middle Aged , PrognosisABSTRACT
Transformation of Drosophila melanogaster using P-element-based vectors yielded 129 sublines, which carried mini-white gene copies in the different genome regions. Dependence of mini-white gene expression on the location, gene dosage, and sex of the transformed individuals was analyzed. The mutation lzb was shown to suppress mini-white gene expression, the degree of suppression depending on the location and dosage of the mini-white gene.
Subject(s)
ATP-Binding Cassette Transporters , DNA Transposable Elements , Drosophila Proteins , Drosophila melanogaster/genetics , Eye Proteins , Transformation, Genetic , Animals , Eye Color/genetics , Female , Gene Dosage , Genetic Vectors , Heterozygote , Homozygote , Insect Hormones/genetics , MaleABSTRACT
P-element-mediated transformation of Drosophila melanogaster was performed and the effectivity of transformation determined using the y+snwsc wa stock. Some new D. melanogaster stocks with sne and sn+ phenotypes were obtained as well as the stocks containing bacterial "neo" gene integrated into the genome.
